The Effects of 3-Methylindole on Hemolysis,Transport of 22 Na +,and ATPase Activities of Bovine Erythrocytes 1

Summary

Biochemical effects of 3MI on cellular membranes were investigated. This study was conducted to examine the effects of 3MI on the hemolysis of erythrocytes, the transport of ²²Na⁺ in resealed erythro-cyte ghosts, and on the ATPase activities of erythrocyte membranes. The percent of hemolysis as a function of 3MI incubation time was sigmoidal. Seventy-five percent of the hemoglobin was released with the second 2 hr of incubation during which the concentration of 3MI in the cells reached a plateau of 2500 μg/ml of packed RBC. The effect of 3MI at a subhemolytic concentration on passive and active ²²Na⁺ transport were not significant. The total and Mg²⁺-dependent ATPase activities in the membranes were significantly increased after 1 hr of incubation with 3MI at concentrations of 100, 200, 300, 400 and 500 μg/ml (P ≤ 0.02).