Abstract
Summary
Liver cells from normal C3H/HeDub mice have reduced activities of mitochondrial succinate dehydrogenase (EC 1.3.99.1), malate dehydrogenase (EC 1.1.1.38), and adenylate kinase (EC 2.7.4.3) after being exposed to 10μg Escherichia coli 0127:B8 lipopolysaccharide (Westphal preparation) per milliliter of culture medium lacking serum for 2 hr at 37°C. Liver cells from endotoxin-refractory mice, whether rendered resistant by prior treatment with LPS, or from the genetically resistant C3H/HeJ mouse, exhibited normal mitochondrial activity after being exposed to LPS. Isolated mitochondria from endotoxin susceptible and endotoxin-refractory mice were sensitive to LPS indicating that resistance is a cellular and not an organellar attribute.
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