The Role of Macrophages in the Chemotactic Response of Polymorphonuclear Leukocytes to Bacterial Lipopolysaccharides 1

Abstract

C3H/HeJ mice do not respond to the toxic and immunological properties of LPS whereas histocompatible C3HeB/FeJ do respond. The unresponsiveness of C3H/HeJ strain is genetically determined and occurs primarily at the cellular level. In the present study, the author investigated the peritoneal PMN influx induced after i.v. LPS administration in C3H/HeJ recipients that received i.p. different C3HeB/FeJ or C3H/HeJ peritoneal cells. It is shown that a significant PMN migration into the peritoneal cavity does occur after i.v. LPS administration only in C3H/HeJ recipients previously inoculated with C3HeB/FeJ unseparated or adherent peritoneal cells. The peritoneal PMN influx after i.v. LPS injection was not observed in C3H/HeJ recipients that received i.p. C3HeB/FeJ nonadherent peritoneal cells or C3H/HeJ unseparated peritoneal cells. Our data suggests that the peritoneal chemotactic signal is triggered after the interaction of LPS with the adherent peritoneal cell population.