Renal Effects of Aprotinin,a Kallikrein Inhibitor in Rats in Saline Diuresis

Abstract

Administration of aprotinin, a kallikrein inhibitor, to anesthetized rats infused with 0.9% saline solution to expand the extracellular fluid volume resulted in blunted natriuresis and diuresis. Urine flow declined from 27.1 ± 2.6 to 8.0 ± 0.9 μl/min/100 g body wt while sodium and potassium excretion were reduced 63 and 45%, respectively (P < 0.01). Mean blood pressure and glomerular filtration rate were not significantly altered by aprotinin. Acute or chronic pretreatment with DOCA, to enhance kinin synthesis, failed to modify the renal excretory response to aprotinin suggesting that saline loading alone was able to induce kinin generation fully in these rats. The results indicate that aprotinin enhanced the reabsorption of filtrate in rats expanded with isotonic saline and imply an influence of renal kinins on the tubular transport Of salt and water.