A New Metabolic Labelling Medium for Trichomonas Vaginalis and Tritrichomonas Foetus Using 35 S methionine

Abstract

A metabolic labelling medium was devised for Trichomonas vaginalis and Tritrichomonas foetus utilizing ³⁵S methionine. T. vaginalis cultured for 24h in the medium took up approximately 27% of the available label and increased greater than two fold in number. Counts per microgram of protein were 32,555 ± 10% between different strains or identical strains in different labelling runs. T. foetus took up approximately 5% of the available label and increased greater than two fold in 24h. This resulted in specific labelling of 12,704 cpm/ug protein ± 10% between different runs with the same strain.