Molecular Cloning of the Complementary DNA for a Human Folate Binding Protein 1

Abstract

The complementary DNA for a human folate binding protein has been cloned from a λgt11-cDNA library prepared from cultured KB cells. A number of clones were selected by immunoscreening with a monospecific antiserum and by oligonucleotide probes corresponding to the NH₂-terminal sequence of the folate binding protein. A partial nucleotide sequence of the cDNA was determined directly from the λgt11 phage and after subcloning into M13. The 18 amino acids deduced from the initial 19 codons were exactly the same as the amino acid sequence obtained by peptide analysis of the purified protein providing proof that this clone is the folate binding protein cDNA.