Role of Vasopressin in Rats with Bilateral Ureteral Obstruction

Abstract

After unilateral release of bilateral ureteral obstruction (BUO), there is a significant increase in renal vasoconstriction that accounts for the marked decrease in glomerular filtration rate and effective renal plasma flow seen in this setting. We examined the potential role of antidiuretic hormone (ADH), a vasoconstrictor of the renal circulation, on renal hemodynamics in female Sprague-Dawley rats with BUO of 24-hr duration. Rats with BUO had significantly higher plasma values of ADH (65.1 ± 12.2 vs 12.1 ± 4.1 pg/ml), sodium (145.4 ± 0.91 vs 138.6 ± 1.06 mEq/liter), and osmolality (375.6 ± 2.0 vs 310.1 ± 3.6 mOsm/kg) than sham-operated rats. Rats with BUO pretreated with enalapril, an angiotensin-converting enzyme inhibitor, before obstruction had somewhat higher, but not significantly different, plasma values for ADH (84.6 ± 20.8 pg/ml) than rats with BUO not given enalapril. Rats with unilateral ureteral obstruction of 24-hr duration had plasma levels of ADH (8.2 ± 1.3) not different from those in sham-operated rats. Rats with BUO pretreated with a specific antagonist of the V₁-type receptor for ADH had significantly greater values for the glomerular filtration rate (2.31 ± 0.24 vs 1.44 ± 0.08 ml/min/kg body wt) and the effective renal plasma flow (8.95 ± 0.71 vs 3.81 ± 0.44 ml/min/kg body wt) and significantly lower values for mean arterial pressure (140.3 ± 2.0 vs 159.1 ± 5.5 mm Hg) than did BUO rats not given the antagonist. The results indicate that high levels of ADH play an important role in the decrease in the glomerular filtration rate and effective renal plasma flow observed in rats with BUO of 24 hr. The significant increase in ADH levels after BUO of 24-hr duration may be due to an increase in osmotic stimulation as a consequence of hypernatremia.