Neural Regulation of Glucose-Stimulated Insulin Secretion in Younger and Older Fischer 344 Rats

Abstract

Neural regulation of insulin secretion of in situ innervated perfused pancreases was evaluated in younger (5 months) and older (26 months) Fischer 344 rats. In one protocol, the central nervous system (CNS) was intact throughout the entire 120-min perfusion period. In the other protocol, the CNS was intact only through the first 20 min of the 120-min perfusion, whereupon the CNS was ablated via anoxia. In both protocols, a modified Krebs-Ringer buffer containing glucose at 200 mg/dl was perfused through the pancreas at a rate of 4.8 ml/min by using a constant flow perfusion pump. Insulin secretion (ng · min^-1) of younger and older CNS-intact rats did not differ significantly. After the ablation of the neural regulation of the pancreas, glucose-stimulated insulin secretion of younger rats was significantly lower, relative to the average insulin secretion before ablation (i.e., min 1–20) of CNS-intact animals. This would suggest that the nature of neural control of insulin secretion in younger rats is potentiation. In contrast, insulin secretion of older CNS-ablated animals was similar, or generally increased, when the data were expressed either on an absolute or a relative basis to preablation values, respectively. Thus, these data suggest that the neural regulation of glucose-stimulated insulin secretion in younger versus older rats is significantly different.