Studies on the Cultivation of Sp. gallinarum.

Conclusion

On the medium described above we have been able to cultivate Sp. gallinarum without difficulty. The cultures grow best at 37°C, but growth occurs also at 30°C and at 39°C; at 30°C growth is slower but the viability better than at 37°C, at 39°C the reverse is the case. The cultures reach optimum growth density in 4 to 5 days according to the size of the inoculum. If transferred to the ice box at this point they remain active for 10 days to 2 weeks. Transplants have been made as late as the 15th day of culture. One strain has been carried through 19 generations during a period of 150 days. The 18th subculture of this strain was still fully virulent for chickens and caused fatal infections.