Physiology of Bacteria-free Trichomonas vaginalis . VII: Temperature in Relation to Survival and Generation Time. ∗

Conclusions

1. Trichomonas vaginalis survives exposure at temperatures near the freezing point for 96 to 144 hours and at 24-2 5 °C for 120 to 154 hours. Exposure to -72 °C for one minute destroys the organisms. 2. The thermal death time for temperatures above 43°C has been recorded. At 50°C it is 4 minutes. 3. The lowest temperature consistent with multiplication is 25°C; the upper limit 41 °C. 4. The minimum time required for cell division is 5 to 8 hours and was observed over a broad range from 34° to 39°C.