Temperature Coefficients of Hemolysis of a Few Types of Nucleated Erythrocytes. ∗

Jacobs et al. 1 have presented a series of data giving temperature coefficients for the hemolysis of various types of mammalian erythrocytes in non-electrolytes. These data have recently been utilized in an attempt to relate species differences in permeability to possible chemical differences in the cell membrane (Dziemian 2 and Ballentine 3 )

Because of their possible use in future studies, and for comparative purposes, the data in Table I are presented giving temperature coefficients of hemolysis of nucleated erythrocytes of several species.

The technic employed in these experiments was essentially that used by Jacobs et al. 1 In general, the temperature coefficients of hemolysis for these nucleated erythrocytes are of the same order of magnitude as those obtained using mammalian red cells. These data are too fragmentary to justify a detailed analysis. However, certain comparisons can well be made with data described by Jacobs and Glassman. 4 These authors point out that in fishes (elasmobranchs and teleosts) the permeability to ethylene glycol is high, in birds the permeability to ethylene glycol and glycerol is very great and nearly equal, and much less to urea, except in the duck and chicken, and permeability to urea is great in reptiles. The present data are in complete agreement. Ethylene glycol permeability is high in the four fishes studied, urea and glycerol permeability is high in the chicken, and the turtle exhibits a very high permeability to urea.