Cytotoxic Action of Antisera to Cell Components of Normal and Leukemic Mouse Spleens. ∗

Summary

Antisera to both normal and leukemic spleen cell components exhibit cytotoxic effects for leukemic cells, upon in vitro exposure. In every instance the leukemic antisera were much more effective than the corresponding normal antisera. These differences were greater than those obtained by complement fixation procedures. The anti-mitochondria sera exhibited the highest “titers” but the least difference between the normal and leukemic. The most significant differences occurred between the leukemic and normal Pi antisera, with the leukemic antiserum showing 8 times the activity of the normal. Highly significant differences were also demonstrated between the nuclei antisera. The leukemic antiserum proved over 6 times as effective as the normal. A slight active immunity was demonstrated in mice surviving the combination of leukemic cells and leukemic antisera.