Activity of Ethyl-l-Ethanesulfonyl-4-Piperazine Hydrochloride on Traumatic Hemorrhagic Shock on the Dog

Researches, in recent years, on traumatic shock therapy, have led us to examine the effects of the following substances on shock induced in the rat by means of the drum technic (Noble and Collip), with polyvinylpyrrolidone (1), some simple aliphatic amides (2), and the protective effect exercised by a new synthetic compound, ethyl-1-ethanesul-fonyl-4-piperazine (3885 R.P.)(3).

In previous experiments we tested the effect of ethyl-l-ethanesulfonyl-4-piperazine (3885 R.P.) on shock produced in the mouse, rat and guinea pig, then decided to carry out further experiments on the dog. We used the technic of Wiggers, Ingraham and Dilles(4) which produced an irreversible shock following a prolonged hemorrhagic hypotension period. The animals were treated as described by Wiggers, under morphine or chloralose anesthesia. By extensive bleeding the blood pressure was lowered to 40 mm of kg maintained for a given time by a series of successive hemorrhages and reinjections. A total reinjection of the removed blood immediately followed the period of hypotension. The wounds were treated locally with sulfanilamide and the surviving animals were given penicillin. To minimize the seasonal or other variations which constitute the principal obstacles in this type of research, the experiments were repeated in the spring and in the autumn, and parallel experiments have been carried out in Paris and in Rome. One hundred dogs were used. The duration of hypotension was prolonged for more than the 90 minutes reported by Wiggers(4), and there resulted a larger proportion of irreversible shock in the control animals.

In the first series of experiments (I, II, III), 16 control dogs were subjected to 2 hours hypotension, with a mortality of 94%. In series IV, the control dogs exhibited a higher resistance and the period of hypotension was extended to 21/2 hours.