Effect of Coconut Milk Fractions on Growth of Carrot Tissue

Summary

Addition of coconut milk (7 mg/ml) to a medium containing 2% sucrose, 1% agar and White's mineral solution supported the growth in darkness of Clone II carrot tissue for over a year through more than 100 transfers. Adsorption of coconut milk on Nuchar followed by elution with chloroform-methanol (5:2) gave two fractions, neither of which would support the growth of this tissue alone, but which, on recombination, had the same activity as the original coconut milk. The effect of the eluate factor could be partially reproduced by indole acetic acid. The filtrate factor could not be replaced by any of the chemically defined substances tested.