Phagocytosis Influenced by Bacterial Culture Medium

Summary

1. The medium in which E. coli is grown influences its phagocytosis. Organisms grown in beef infusion are phagocytosed much more than those grown in tryptose medium. 2. The factor(s) in beef infusion is ultrafilterable and stabel to autoclaving. 3. The absence of opsonin from beef infusion or from supernatnat of beef infusion cultures, and the apearance of sensitization during growth, indicate formation of sensitizing substances by E. coli in beef infusion medium.