Growth of Poliomyelitis Viruses in Large Stationary Cultures. ∗

Summary

Poliomyelitis viruses were propagated in large stationary tissue cultures of plasma imbedded monkey testes. Large volumes of supernatant fluids with high virus titers were achieved. The virus yield, as measured by titer and duration of maximum growth, was almost identical with that obtained in roller tubes, and greater than that reported for suspended cell flask cultures. The method had another advantage in that equipment for rotating bottles was not required. Since the Roux flasks could be stacked one on top of another, a minimum of incubator space was required.