Effect of Exposure to a Gold Environment on Labeling of Phospholipide in Rat Liver Slices. ∗

Summary

In rat liver slices respiring in a buffered medium containing suitable radioactive precursors, the acetone-insoluble lipide (phospholipide) is labeled metabolically. Exposure of the animals to an environmental temperature of 4–5° C for the 12 days prior to the experiment caused a decrease in the subsequent labeling of the phospholipide from acetate- 1 - C¹⁴, but no significant change when inorganic P³² was the source of the radioactivity. Exposure of the rats to — 5 ° C for the 3 hours immediately prior to the experiment caused a decrease in the labeling of the phospholipide when the radioactivity was derived from acetate-1-C¹⁴, but no change when it was derived from glycerol-1-C¹⁴, glycine-2-C¹⁴ or inorganic P³².