Abstract
Summary
A strain of rabbit fibroblasts designated strain RM3–73 requires folic acid, nicotinamide, pantothenic acid, pyridoxal, riboflavin, and thiamine for continuous proliferation in a medium containing 2% (v/v) dialyzed horse serum. The concentration of each vitamin which permits optimal proliferation was determined by propagating the cells serially in media containing graded concentrations of the compound. A group of eight amino acids which are not required for serial propagation in the presence of pyridoxal replaced the requirement for this vitamin under experimental conditions employed.
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