Effects of Superimposed Native Insulin on Disposal of Iodoinsulin in the Body. ∗ †

Summary

In eviscerated-nephrectomized rabbits the decay of iodoinsulin is retarded by addition of moderate and physiological doses of native insulin. The apparent volume of distribution of iodoinsulin is reduced by superimposed native insulin. This suggests a sequestering of part of the tracer compound by a process that can be saturated by added native insulin. The results indicate that in studying the physiology of insulin by use of iodoinsulin, investigators should add native insulin in appropriate dosage. Determinations carried out in this way indicate that iodoinsulin distributes in the eviscerated-nephrectomized rabbit in a volume equal to the extracellular compartment.