Abstract
Summary
The possible use of growth curves in assay procedures for mammary-gland growth promoting substances was investigated. Male mice were fed diethylstilbestrol for 4 weeks to insure duct growth. In addition, one group was injected subcutaneously with 0.75 μg estradiol benzoate (E.B.)/day along with various levels of progesterone (P) for 5- or 10-day periods. Another group similarly received 0.50 μg E.B./day plus various amounts of P for a 10-day period. Desoxyribosenucleic acid determined by a newer, shortened procedure was used as a growth index. Results indicated that best slope with greatest precision was obtained when 0.75 μg E.B./day with various amounts of progesterone was injected for 10-day period.
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