Evaluation of APOE ɛ2/ɛ3/ɛ4 Alleles in a Cohort of Individuals Affected by Developmental Topographical Disorientation

INTRODUCTION

The gene APOE codes for the lipid-binding protein apolipoprotein E (ApoE), which binds to cholesterol and other lipids, and transports them between various cell and tissue types. ApoE is known to be involved in a wide range of processes, including lipoprotein metabolism, immunoregulation, and, importantly, cognition [1–3]. ApoE is associated with Alzheimer disease (AD), representing the main genetic factor determining individuals’ risk of occurrence of AD [2, 4–8]. There are three common isoforms of ApoE (ApoE2, ApoE3, and ApoE4), corresponding to three alleles of APOE (ɛ2, ɛ3, ɛ4), as defined by two single nucleotide polymorphisms at positions 2059 and 2179, affecting the amino acids encoded at positions 112 and 158 of the ApoE protein (Table 1; [3, 10]). These polymorphisms affect the structure and function of the ApoE protein, with the different isoforms exhibiting altered lipid association and receptor binding characteristics [9]. E3 is the isoform most commonly seen in the population, while the E4 and E2 isoforms are associated with an increased and decreased risk for AD, respectively [5] (see [2] for a comprehensive review of APOE/ApoE).

One important characteristic of AD is that patients get lost in familiar surroundings [11]. Such impairment is distinct from the general memory issue that is hallmark of the disease [12] and is present in nearly all AD patients from early on in the disease course [13]. This peculiar behavioral defect in AD patients raised the issue of specific effects that the E2/E3/E4 ApoE isoforms may have on the ability of the individuals to orient and navigate in the environment. Indeed, it has been shown that the E4 allele has an effect on brain activation during memory tasks in healthy adults [14], as well as on spatial memory behavior in healthy children, decades before AD might occur [15]. In searching for early indicators and mechanisms of AD’s neurocognitive pathology in young adults carrying the ɛ4 APOE allele (individuals who are therefore at risk of developing AD later in life), Kunz and colleagues [16] found evidence of a dysfunction of grid cells in the entorhinal cortex, a cell type well-known to be involved in the representation of a spatial layout and especially critical for effective orientation and navigation [17]. Specifically, in the ɛ4 carriers, the authors observed a reduction of grid-cell-like representations while undergoing a functional magnetic resonance imaging (fMRI) study, and a declined performance in spatial orientation while navigating a virtual arena. These authors also found that both of these changes were related to impaired spatial memory performance, although, as a group, the ɛ4 carriers did not exhibit reduced spatial memory when compared to participants homozygous for the ɛ3 allele.

In a different study, Konishi and colleagues also provided evidence of a significant link between the ApoE isoforms and spatial orientation, in which navigational behavior and functional and structural brain properties appeared to be influenced by APOE genotype in healthy young adults [18, 19]. Specifically, these authors found that ɛ2 carriers favored a navigation strategy dependent on the hippocampus (i.e., a spatial over a non-spatial/response strategy), and had increased gray matter volume of the hippocampus, as compared to ɛ4 carriers or participants homozygous for ɛ3, i.e., non-ɛ2 and non-ɛ4 carriers [18]. Later, in comparing ɛ4 carriers who favored a spatial navigation strategy (ɛ4-spatial) to ɛ4 carriers who favored a non-spatial navigation strategy (ɛ4-response), the authors found that ɛ4-response participants had a decreased gray matter volume compared to non-ɛ4 carriers, whereas ɛ4-spatial participants did not (i.e., their gray matter volumes were comparable to non-ɛ4 carriers). In addition, ɛ4-response participants had a decreased blood-oxygen-level-dependent signal (i.e., the indirect fMRI measure of neural activity) when compared to ɛ4-spatial participants [19]. Taken together, these studies suggest that the APOE gene might be a critical genetic determinant of spatial orientation abilities in the healthy population as well as among AD patients. Here, we sought to investigate the APOE ɛ2/ɛ3/ɛ4 genotypes in a group of individuals who suffer from a lifelong inability to orient and navigate in familiar surroundings, that is, individuals affected by developmental topographical disorientation (DTD) [20].

DTD refers to a highly-selective lifelong impairment in the ability of an individual to find their way around in extremely familiar surroundings such as the neighborhood where they live, the building where they have worked for many years, or in extreme cases, their own house [20–25]. This disability, which can be devastating, is present despite the fact that these individuals have well-preserved cognitive functions, no other neurological impairments and no brain injuries [26–28]. This behavioral defect appears most commonly to result from the inability of the individuals to form cognitive maps [21, 29]. A cognitive map is a mental representation of the environment including the landmarks within it and their spatial relationships and is formed as individuals familiarize themselves with their spatial surroundings, enabling them to reach any location from anywhere within the environment [30, 31]. Individuals affected by DTD are unable to form cognitive maps, and therefore never gain the ability to orient within a given environment, no matter how long they live in it [29]. Importantly, there is evidence found in a cohort of individuals with DTD of familial clustering of this trait, suggesting that genes might play a role in the development of DTD [20, 32].

The evidence that the three common APOE alleles may play a role in determining spatial orientation ability marks them as potential candidates for genetic variants contributing to the occurrence of DTD. Specifically, DTD may be more common among ɛ4 carriers, given the evidence of their grid cell dysfunction [16] and increased risk of developing AD. Also, DTD may be less common in ɛ2 carriers, given the evidence of a more likely use of a hippocampal effective spatial navigation strategy [18]. To test these hypotheses, we genotyped the common APOE alleles in a cohort of DTD individuals and their families.

MATERIALS AND METHODS

RESULTS

Among the 20 unrelated DTD probands, we observed 16 individuals with the ɛ3/ɛ3 genotype; two with ɛ2/ɛ3; one with ɛ3/ɛ4; and one with ɛ4/ɛ4 genotypes (Table 2). This pattern of allele frequencies did not appear to differ from that expected in the general population (χ²(2) = 2.340, p = 0.309), but were distinct from that expected in an AD population (χ²(2) = 15.642, p <  0.001), see Table 3 and Fig. 1. Additionally, when the less common ɛ2 and ɛ4 alleles were observed within a family, they did not co-segregate with either the DTD or non-DTD phenotype (Table 2).

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Fig. 1

Allele proportions of the present developmental topographical disorientation (DTD) cohort, as well as reference proportions from Caucasian control and Alzheimer’s disease (AD) populations [5]. The shaded area about the DTD point estimate represents a 95%confidence interval, estimated from independent binomial distributions.

DISCUSSION

To investigate whether APOE genetics was involved in the development of DTD, we determined the ɛ2/ɛ3/ɛ4 genotype in 20 unrelated individuals with DTD and found that the allele frequencies in this DTD group were similar to those seen in the general population (Table 3). These results indicate that the three major APOE isoforms do not determine DTD status, suggesting that if there is a genetic component to DTD, this would involve some other gene(s). Therefore, while evidence exists that APOE is involved in the development of spatial orientation abilities [16, 19], other proteins/genes are likely involved as well. Our findings show that DTD can occur against a genetic background containing any of the three ɛ2/ɛ3/ɛ4 alleles. In other words, individuals with any of the three major APOE isoforms can have a total lack of developed spatial orientation abilities. Additionally, in an exome sequencing study of nine of these DTD probands, we did not identify rare protein-altering variants in APOE. Therefore, APOE cannot be the single major genetic determinant of spatial orientation abilities in humans. This is not at all surprising since most gene products work together with several (or many) other gene products in complex interaction networks in order to carry out their biological functions. This would especially be expected when looking at such a complex function as spatial orientation, which is already known to involve several brain regions and several cognitive processes. If a major genetic determinant of DTD is discovered in the future, it may be important to identify whether or not it interacts with APOE in any way.

Another way to investigate a possible relationship between APOE genetics and DTD would be to document the incidence of DTD and the ɛ2/ɛ3/ɛ4 genotype in a large population of individuals. In the future, such a large-scale study could eventually allow for a more definitive statement about whether ɛ2/ɛ3/ɛ4 genotype contributes to DTD risk. Indeed, such a large-scale study would allow for the investigation of all potential genetic contributors to the development of DTD. Here, in a small study of a DTD population, we have seen that the pattern of ɛ2/ɛ3/ɛ4 genotypes does not appear to differ from what would be expected in any population of this size, indicating, preliminarily, that ɛ2/ɛ3/ɛ4 genotype is at least not the major genetic determinant of DTD.

Interestingly, among individuals with the ɛ4 allele, brain changes appeared to be linked to navigation strategy [19]. ɛ4 carriers who used a non-spatial strategy were more likely to have the brain changes expected to be indicative of AD risk [19]. Individuals with DTD, being impaired in their ability to form cognitive maps, must rely on a non-spatial (e.g., a response) strategy to navigate. Therefore, future studies may focus on investigating the frequency of developing AD in DTD individuals, as well as the use of navigational strategies aimed at improving the spatial orientation skills in those with DTD, which may protect against AD-type brain changes in ɛ4 carriers. It would be critical to answer these questions in order to start defining the relationship between AD and the presence of a lifelong condition such as DTD.

Our study was limited by the sample size. Indeed, even if no DTD probands had the ɛ2 allele, our sample size would not be big enough to say that the ɛ2 allele frequency was significantly lower in our cohort than in the general population. However, our results show a similar pattern of APOE alleles in the DTD group as is seen in the general population, and, as such, any effect, if present, is going to be subtle. Therefore, future studies in a larger DTD cohort will be beneficial to continue exploring the genetics of the DTD trait, and will reveal whether or not such a subtle effect does indeed exist.