Endothelial microparticles (EMP) are small membrane vesicles that originate from activated or apoptotic endothelial cells. Although the exact mechanism of EMP function is still relatively unknown, it has been shown that they modulate inflammatory processes, coagulation and vascular function. In this study we hypothesized that transient hypoxia may act as a trigger for the release of EMP into circulation.
MATERIALS AND METHODS:
Fourteen healthy volunteers were subjected to transient normobaric hypoxia in an air-conditioned chamber simulating an oxygen concentration of a height of up to 5500 meters. Blood samples were evaluated for EMP using flow cytometry.
RESULTS:
During the experiment oxygen concentration was adjusted to a value equivalent to a height of 5500 meters to achieve hypoxic conditions. Oxygen saturation decreased to 78% . At the final height a significant increase of CD31+/Annexin+ EMP levels was evident (increase from 0.03% ± 0.01% SEM to 0.12% ± 0.04% SEM, p = 0.0188).
CONCLUSIONS:
These experimental results show that temporary hypoxic conditions can trigger the release of CD31+/ Annexin+ EMP also in healthy volunteers. In our previous studies we have shown that apoptotic bodies can confer pro-survival signals to cardiomyocytes during myocardial ischemia. Based on the experimental results of this current study we believe that the release of CD31+/Annexin+ EMP during hypoxia might act as an endogenous survival signal.
Bernal-MizrachiLJyWJimenezJJPastorJMauroLMHorstmanL.L2003High levels of circulating endothelial microparticles in patients with acute coronary syndromesAm Heart J145962970
4.
BlannAShantsilaEShantsilaA2009Microparticles and disease arterial, Semin ThrombHemost35488496
5.
BrillADashevskyORivoJGozalYVaronD2005Platelet-derived microparticles induce angiogenesis and stimulate post-ischemic revascularizationCardiovasc Res673038
6.
ChirinosJAHeresiGAVelasquezHJyWJimenezJJAhnE2005Elevation of endothelial microparticles, platelets, and leukocyte activation in patients with venous thromboembolismJ Am Coll Cardiol4514671471
7.
CombesVSimonACGrauGEArnouxDCamoinLSabatierF1999In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulantJ Clin Invest10493102
8.
FritzenwangerMJungCGoebelBLautenAFigullaHR2011Impact of short-term systemic hypoxia on phagocytosis, cytokine production, and transcription factor activation in peripheral blood cellsMediators Inflamm2011429501
9.
GoebelBHandrickVLautenAFritzenwangerMSchutzeJOttoS2013Impact of acute normobaric hypoxia on regional and global myocardial function: A speckle tracking echocardiography studyInt J Cardiovasc Imaging29561570
10.
IshiharaYSekineMHatanoAShimamotoN2008Sustained contraction and endothelial dysfunction induced by reactive oxygen species in porcine coronary arteryBiol Pharm Bull3116671672
11.
JungCQuitterFLichtenauerMFritzenwangerMPfeilAShemyakinAIncreased arginase levels contribute to impaired perfusion after cardiopulmonary resuscitationEur J Clin Invest2014
12.
JungCRafnssonAShemyakinABohmFPernowJ2010Different subpopulations of endothelial progenitor cells and circulating apoptotic progenitor cells in patients with vascular disease and diabetesInt J Cardiol143368372
13.
JungCSorenssonPSalehNArhedenHRydenLPernowJ2012Circulating endothelial and platelet derived microparticles reflect the size of myocardium at risk in patients with ST-elevation myocardial infarctionAtherosclerosis221226231
14.
LichtenauerMMildnerMBaumgartnerAHasunMWerbaGBeerL2011Intravenous and intramyocardial injection of apoptotic white blood cell suspensions prevents ventricular remodelling by increasing elastin expression in cardiac scar tissue after myocardial infarctionBasic Res Cardiol106645655
15.
LichtenauerMMildnerMHoetzeneckerKZimmermannMPodesserBKSiposW2011Secretome of apoptotic peripheral blood cells (APOSEC) confers cytoprotection to cardiomyocytes and inhibits tissue remodelling after acute myocardial infarction: A preclinical studyBasic Res Cardiol10612831297
16.
LichtenauerMZimmermannMNicklSLautenAGoebelBPistulliR2014Transient hypoxia leads to increased serum levels of heat shock protein-27, -70 and caspase-cleaved cytokeratin 18Clin Lab60323328
17.
MarkiewiczMRichardEMarksNLudwicka-BradleyA2013Impact of endothelial microparticles on coagulation, inflammation, and angiogenesis in age-related vascular diseasesJ Aging Res2013734509
18.
Martinez-SalesVVilaVRicartJMVayaATodoliJNunezC2013Increased circulating endothelial cells and microparticles in patients with psoriasisClin Hemorheol Microcirc
19.
MichelsenAEBrodinEBrosstadFHansenJB2008Increased level of platelet microparticles in survivors of myocardial infarctionScand J Clin Lab Invest68386392
20.
MildnerMHackerSHaiderTGschwandtnerMWerbaGBarresiC2013Secretome of peripheral blood mononuclear cells enhances wound healingPLoS One8e60103
21.
MudauMGenisALochnerAStrijdomH2012Endothelial dysfunction: The early predictor of atherosclerosisCardiovasc J Afr23222231
22.
PirroMSchillaciGPaltricciaRBagagliaFMenecaliCMannarinoM.R2006Increased ratio of CD31+/CD42- microparticles to endothelial progenitors as a novel marker of atherosclerosis in hypercholesterolemiaArterioscler Thromb Vasc Biol2625302535
23.
PriouPGagnadouxFTesseAMastronardiMLAgouniAMeslierN2010Endothelial dysfunction and circulating microparticles from patients with obstructive sleep apneaAm J Pathol177974983
24.
QuitterFFigullaHRFerrariMPernowJJungC2013Increased arginase levels in heart failure represent a therapeutic target to rescue microvascular perfusionClin Hemorheol Microcirc547585
25.
SabatierFCamoin-JauLAnfossoFSampolJDignat-GeorgeF2009Circulating endothelial cells, microparticles and progenitors: Key players towards the definition of vascular competenceJ Cell Mol Med13454471
26.
SchramMTStehouwerCD2005Endothelial dysfunction, cellular adhesion molecules and the metabolic syndromeHorm Metab Res37Suppl 14955
27.
ShanLYLiJZZuLYNiuCGFerroAZhangYD2013Platelet-derived microparticles are implicated in remote ischemia conditioning in a rat model of cerebral infarctionCNS Neurosci Ther19917925
28.
SimakJGeldermanMPYuHWrightVBairdAE2006Circulating endothelial microparticles in acute ischemic stroke: A link to severity, lesion volume and outcomeJ Thromb Haemost412961302
29.
SoletiRMartinezMC2012Sonic Hedgehog on microparticles and neovascularizationVitam Horm88395438
30.
SorianoAOJyWChirinosJAValdiviaMAVelasquezHSJimenezJJ2005Levels of endothelial and platelet microparticles and their interactions with leukocytes negatively correlate with organ dysfunction and predict mortality in severe sepsisCrit Care Med3325402546
31.
ThomasDGiuglianoRP2009Management of ST-segment elevation myocardial infarction: Comparison of the updated guidelines from North America and EuropeAm Heart J158695705
32.
TrzepizurWPriouPParisANardiJTual-ChalotSMeslierN2011Nocturnal release of leukocyte-derived microparticles in males with obstructive sleep apnoeaEur Respir J3712931295
33.
UrschelKCichaIDanielWGGarlichsCD2012Shear stress patterns affect the secreted chemokine profile in endothelial cellsClin Hemorheol Microcirc50143152
34.
VelagaletiRSPencinaMJMurabitoJMWangTJParikhNID’AgostinoRB2008Long-term trends in the incidence of heart failure after myocardial infarctionCirculation11820572062
35.
VinceRVChrismasBMidgleyAWMcNaughtonLRMaddenLA2009Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophilsOxid Med Cell Longev226
36.
WangJChenSMaXChengCXiaoXChenJ2013Effects of endothelial progenitor cell-derived microvesicles on hypoxia/reoxygenation-induced endothelial dysfunction and apoptosisOxid Med Cell Longev2013572729
37.
ZhangMMalikABRehmanJ2014Endothelial progenitor cells and vascular repairCurr Opin Hematol21224228
38.
ZielinskaMKoniarekWGochJHCebulaBTyburaMRobakT2005Circulating endothelial microparticles in patients with acute myocardial infarctionKardiol Pol62531542discussion 43-4
