Effect of rejuvenation of blood on 2,3-diphosphoglycerate,hemoglobin-oxygen affinity and filtrability of red blood cells

Filtrability of erythrocytes through polycarbonate sieves with a mean pore diameter of 5 m was studied in fresh blood and stored donor blood before and after rejuvenation with PIGPA and the result was related to simultaneous variations in red cell 2,3-diphosphoglycerate content (2,3-DPG). Following incubation with PIGPA for 2 hours there occurred a significant increase in the content of red cell 2,3-DPG reaching the highest values in fresh blood (14.85 ± 1.05 vs. 4.80 ± 0.65 mmol/l eryth., p < 0.001). The oxygen affinity of hemoglobin simultaneously decreased, and the P₅₀, 7.40 closely correlated with red cell 2,3-DPG content (r = 0.86, p < 0.001). Erythrocyte filtrability of all samples with excess 2,3-DPG content significantly decreased and a negative correlation was present between the concentration of red cell 2,3-DPG and the flow rate (r = −0.67, P < 0.001).