Abstract
Many researches show that traditional Indonesian diet is good for physical health. The present study examines the antioxidative, anti‐inflammatory and antithrombotic potentials of aqueous extract of tempe (fermented soya‐beans) and Curcuma domestica in an experimental photochemical thrombogenesis model using rat femoral artery. A total of 15 male Wistar rats weighing 250 g were used, and divided into three groups: control (group‐1, n=5), animals, treated orally with 0.25 ml aqueous extracts of 50 g tempe (fermented soybean cake) once daily for 7 consecutive days (group‐2, n=5) and animals treated orally with 0.25 ml aqueous extracts of 10 g roots of Curcuma domestica once daily for 5 consecutive days (group‐3, n=5). All animals were anesthetized, and Sn‐pyrophosphate and Tc99m solutions were injected intravenously for in vivo red cell radioactive labeling. Femoral arterial occlusion was observed, using a gamma camera. Induction of femoral arterial thrombosis was effected following an endothelial injury by free radicals produced by green light‐irradiated Rose Bengal (10 mg/kg). The results showed that in the control group arterial total flow occlusion was seen in 15 min of irradiation. The results of MDA absorbency was 0.3700±1.7 nmol/ml in control group‐1, 0.0520±0.025 in group‐2 (significant p<0.05 in comparison to control group) and 0.2780±0.027 in group‐3 (non‐significant). Interleukin‐1alpha plasma level was 14.44±2.3 in control group‐1, 8.93±2.4 in group‐2 (significant p<0.05), and 6.21±2.5 in group‐3 (significant p<0.05). Plasma thromboxane B2 plasma level was 20.31±2.4 in control group‐1, 14.32±2.2 in group‐2 (significant p<0.05), and 19.41±2.1 in group‐3 (significant). This study suggests the potential antioxidative, anti‐inflammatory and antithrombotic effect that the dietary aqueous extracts has in rat femoral artery.