Induction of Amyloid-β 42 Production by Fipronil and Other Pyrazole Insecticides

Abstract

Generation of amyloid-β peptides (Aβs) by proteolytic cleavage of the amyloid-β protein precursor (AβPP), especially increased production of Aβ₄₂/Aβ₄₃ over Aβ₄₀, and their aggregation as oligomers and plaques, represent a characteristic feature of Alzheimer’s disease (AD). In familial AD (FAD), altered Aβ production originates from specific mutations of AβPP or presenilins 1/2 (PS1/PS2), the catalytic subunits of γ-secretase. In sporadic AD, the origin of altered production of Aβs remains unknown. We hypothesize that the ‘human chemical exposome’ contains products able to favor the production of Aβ₄₂/Aβ₄₃ over Aβ₄₀ and shorter Aβs. To detect such products, we screened a library of 3500 + compounds in a cell-based assay for enhanced Aβ₄₂/Aβ₄₃ production. Nine pyrazole insecticides were found to induce a β- and γ-secretase-dependent, 3-10-fold increase in the production of extracellular Aβ₄₂ in various cell lines and neurons differentiated from induced pluripotent stem cells derived from healthy and FAD patients. Immunoprecipitation/mass spectrometry analyses showed increased production of Aβs cleaved at positions 42/43, and reduced production of peptides cleaved at positions 38 and shorter. Strongly supporting a direct effect on γ-secretase activity, pyrazoles shifted the cleavage pattern of another γ-secretase substrate, alcadeinα, and shifted the cleavage of AβPP by highly purified γ-secretase toward Aβ₄₂/Aβ₄₃. Focusing on fipronil, we showed that some of its metabolites, in particular the persistent fipronil sulfone, also favor the production of Aβ₄₂/Aβ₄₃ in both cell-based and cell-free systems. Fipronil administered orally to mice and rats is known to be metabolized rapidly, mostly to fipronil sulfone, which stably accumulates in adipose tissue and brain. In conclusion, several widely used pyrazole insecticides enhance the production of toxic, aggregation prone Aβ₄₂/Aβ₄₃ peptides, suggesting the possible existence of environmental “Alzheimerogens” which may contribute to the initiation and propagation of the amyloidogenic process in sporadic AD.

Keywords

aftins Alzheimer’s disease alzheimerogen amyloid-β amyloid-β protein precursor fipronil γ-secretase human chemical exposome pesticides phenylpyrazoles prevention pyrazoles triazines

INTRODUCTION

Alzheimer’s disease (AD) is a major disease in countries with aging populations. Despite unresolved questions on the initial causes of AD and numerous clinical trial failures in recent years, the lack of satisfactory treatments and the extremely high prevalence of AD calls for fundamental research to determine its underlying molecular and cellular causes and mechanisms and for applied research to identify options for prevention, therapeutic targets and disease-modifying drug candidates (reviews in [1 –4]).

Although the aggregation of specific forms of amyloid-β peptides (Aβs) into soluble oligomers is undoubtedly associated with the onset of AD, the upstream etiological events underlying this pathological process remain unclear, including whether there is an absolute, or relative, increase in the production of longer, aggregation-prone Aβ species. Aβs are derived from the successive action of two proteases, β-secretase and γ-secretase, on one of their numerous substrates, the transmembrane amyloid-β protein precursor (AβPP). According to its cleavage site, γ-secretase liberates Aβs of different sizes. While Aβ₄₀ is considered as the main physiological product, the appearance of Aβ₄₂ and Aβ₄₃, or increase of the Aβ₄₂-Aβ₄₃/Aβ₄₀ ratio is clearly associated with the onset of AD [5 –7]. These longer Aβs show an increased propensity to form oligomers, which can assemble into large extracellular deposits, the amyloid plaques, a characteristic feature of AD. Aβ₄₂/Aβ₄₃ oligomers are now considered as the toxic, AD initiating elements rather than the more prominent plaques initially discovered by Aloïs Alzheimer. This ‘amyloid cascade theory’ is further supported by the identification of the genetic causes of early onset familial AD (FAD). These rare forms of AD (less than 1% of all AD cases) are indeed all associated with specific mutations of either AβPP or PS1 or PS2 (Presenilins) genes (the latter encode the catalytic subunits of the γ-secretase complex) (review in [8, 9]). In addition, a specific, protective mutation of AβPP was recently found to be associated with reduced risk for developing AD [10]. Furthermore, animal models that express mutated forms of AβPP and/or PS1, or human Aβ₄₂/Aβ₄₃, develop molecular, cellular and cognitive deficits reminiscent of AD ([6], review in [11, 12]). Yet, the level of the different forms of Aβs is not well correlated with cognitive deficits in mice, suggesting that Aβs may represent markers rather than inducers of AD in these models [13]. An altered processing of γ-secretase substrates other than AβPP might also be important in the pathogenesis of AD. Although FAD is clearly a consequence of genetic mutations of genes encoding the proteins responsible for the production of Aβs, the initial trigger(s) of late-onset, sporadic AD (>99% of AD cases) remain unknown, despite extensive genome-wide association studies (GWAS) and the identification of various risk factors (review in [14]).

Environmental neurotoxic agents including pesticides, organic solvents, metals and some natural toxins (cyanobacteria) are likely sources of AD-inducing factors (reviews in [15 –25]). According to the US Environmental Protection Agency (EPA) Toxic Substances Control Act, over 84,000 chemicals are manufactured or imported at levels >10 tons per year, not including pesticides, cosmetics, food stuffs, and food additives which are covered by other legislations (http://www.epa.gov). It is estimated that we are exposed to over 85,000 substances which, along with all natural substances to which we are exposed from conception to death, constitute the ‘human chemical exposome’ (HCE) [26 –34]. In search for potential “Alzheimerogens” (named by analogy with carcinogens), and encouraged by the discovery of Aftins (Amyloid β Forty-Two Inducers) [35 –37], a class of synthetic compounds which specifically induce the production of Aβ₄₂, we have started to assemble and screen a library of compounds, belonging to the HCE, for Aβ₄₂-inducing products. Our first study identified some triazine herbicides as products stimulating the production of Aβ₄₂/Aβ₄₃ in numerous cell lines [38].

In this new study, we show that several members of the pyrazole class of insecticides, exemplified by fipronil, also increase the γ-secretase-dependent production of Aβ₄₂/Aβ₄₃ peptides in various cell lines and in a cell-free system with highly purified γ-secretase. Interestingly fipronil is metabolized to fipronil sulfone, a very persistent product that accumulates in adipose tissue and brain. These results show that the HCE contains various brain permeable products able to induce the production of pathogenic Aβs. If long exposures occur in real life and if accumulation of Aβ₄₂/Aβ₄₃ is sufficient to trigger neurodegeneration/neuroinflammation, such products may contribute to the initiation, development and acceleration of sporadic AD and might thus be collectively qualified as potential “Alzheimerogens”. Some of them might also be used to develop useful chemically-induced animal models of AD.

MATERIAL AND METHODS

Material and methods are described in full in the Supplementary Material They include: 1) Pyrazoles and other reagents; 2) Cell cultures: cell lines, iPSCs-derived neurons and HEK293-alcadeinα cells; 3) Purified γ-secretase preparation; 4) Aβ cell-based and cell-free assays; 5) Cell viability assay; 6) Pharmacokinetics studies; 7) Proteomics study.

RESULTS

Screening HCE compounds reveals pyrazole insecticides as Aβ₄₂ inducers

We first screened a library of over 3,500 low molecular weight compounds representative of the HCE for their ability to induce the production and secretion of Aβ₄₂ by N2a cells stably expressing AβPP695 (N2a-AβPP695). An MTS-based cell viability assay was run in parallel to assess cell survival. Although the vast majority of compounds were unable to induce Aβ₄₂ production, a few active products including several triazine herbicides [38] and pyrazole insecticides (this article) were identified. We next assembled a small library of 18 pyrazoles (1–18, Supplementary Tables 1 and 2) that we further tested, along with aftin-5 (19) as a positive control, for their ability to trigger Aβ₄₂ production at 5, 10, 25, or 50μM, in both N2a and CHO cells stably expressing AβPP695 and AβPP751, respectively (Supplementary Table 2 and Fig. 1). Nine pyrazoles were found to induce more than a 3-fold change in Aβ₄₂ levels, showing that Aβ₄₂ induction is an intrinsic property of some, but not all, pyrazoles: fenpyroximate (2), tebufenpyrad (3), bixafen (4), fluxapyroxad (5), isopyrazam (6), penthiopyrad (8), chlorantraniliprole (14), tolfenpyrad (16) and fipronil (18) (Figs. 1 and 2). We next focused on fipronil (18) 2 , a widely produced insecticide used to treat crops and pet parasites (reviews in [39 –44]), and we assembled a small library of fipronil analogues and metabolites (Fig. 3) which were tested for their Aβ₄₂ production ability in N2a-AβPP695 cells (Supplementary Table 3). The two fipronil enantiomers (18a, 18b) were separated by SFC chiral chromatography (Regis Whelk O1 SS column) and found to be roughly equipotent. Interestingly the main metabolites of fipronil (18) [45], fipronil sulfone (20), fipronil desulfinyl (21) and fipronil sulfide (22) displayed Aβ₄₂ inducing activity equivalent or close to that of the parent compound. In contrast, a few other environmental metabolites such as fipronil chloramine (24) [46] and hydroxy-fipronil (25) [47] were found to be inactive (Supplementary Table 3).

Fig.1

Some pyrazoles trigger the production of extracellular amyloid Aβ₄₂. Effect of 18 pyrazoles on extracellular amyloid Aβ₄₂ production by N2a-APP695 and CHO-7PA2-APPsw cells. Cells were treated with 100μM of each compound for 18 h and cell supernatants were collected for extracellular Aβ₄₂ levels measurement by an ELISA assay. Aftin-5 was used as a positive control and the corresponding volume of vehicle (DMSO) was used as a negative control. Levels are expressed as fold change, ± SE, of Aβ₄₂ levels over the Aβ₄₂ level of control, vehicle-treated cells. Average of five experiments performed in triplicate. Horizontal dotted lines indicate levels for 1- and 3- fold increases in Aβ₄₂ concentration.

Fig.2

Molecular structure of the nine active pyrazoles.

Fig.3

Molecular structure of fipronil and some of its metabolites and derivatives.

Table 1

Concentrations of fipronil sulfone reached in mammals following fipronil administration (oral, ip, iv, general exposure). Fipronil sulfone (20) levels are expressed either in μg/mL or in μg/g. A 4.53μg/mL concentration is equivalent to a concentration of 10μM. bw, body weight

Species/tissue	Exposure conditions	Fipronil sulfone (20) levels reached	Refs.
Mouse
plasma	10 mg/kg bw, oral, single administration	1–1.1μg/mL	Fig. 11, 12A
plasma	10 mg/kg bw, oral, 5 days/week/ 3 weeks	3.7μg/mL	Fig. 12B
plasma	2.5 or 10 mg/kg bw, oral, 1 day/week/2 weeks	0.029 or 0.210μg/mL	SK, unpubl.
plasma	2.5 or 10 mg/kg bw, oral, 3 days/week/2 weeks	0.211 or 0.662μg/mL	SK, unpubl.
adipose tissue	2 mg/kg bw, ip, daily/6 days	22–24 ppm	[102]
adipose tissue	10 mg/kg bw, oral, single administration	4.3–4.9μg/epididymal adipose pad	Fig. 11, 12A
adipose tissue	10 mg/kg bw, oral, 5 days/ week/ 3 weeks	13.5μg/epididymal adipose pad	Fig. 12B
adipose tissue	2.5 or 10 mg/kg bw, oral, 1 day/week/2 weeks	0.730 or 5.276μg/g	SK, unpubl.
adipose tissue	2.5 or 10 mg/kg bw, oral, 3 days/week/2 weeks	6.098 or 12.48μg/g	SK, unpubl.
brain	40 mg/kg bw, ip, 6 or 20 min, single administration	19 or 32 ppm (6 and 20 min, respectively)	[103]
brain	10 mg/kg bw, oral, single administration	0.71–0.75μg/brain	Fig. 11, 12A
brain	10 mg/kg bw, oral, 5 days/week/ 3 weeks	2.7μg/brain	Fig. 12B
brain	2.5 or 10 mg/kg bw, oral, 1 day/week/2 weeks	0.049 or 0.405μg/g	SK, unpubl.
brain	2.5 or 10 mg/kg bw, oral, 3 days/week/2 weeks	0.585 or 1.746μg/g	SK, unpubl.
Rat
plasma	10 mg/kg bw, oral, single administration	1.4μg/mL	Suppl. Fig. 1
plasma	5 or 10 mg/kg bw, oral, daily/2 weeks	2.4 or 3.6μg/mL	[104]
plasma	30 mg Regent ®/kg bw, oral, daily/15 days	0.46μg/mL	[105]
plasma	4μg/kg bw, oral, 3X/ week/90 days	0.008–0.39 ng/mL	[106]
plasma	3.4 (po) or 6.9 (iv) μmole/kg bw, single administration	0.2 (po) or 0.25 (iv) μg/mL	[107]
plasma	3.4μmole/kg bw, oral, daily/14 days	1.5μg/mL	[107]
plasma	3 mg/kg bw, oral, daily/13 days	0.043 –2.8μg/mL	[108]
adipose tissue	10 mg/kg bw, oral, single administration	152.3μg/g	Suppl. Fig. 3
adipose tissue	10 mg/kg bw, oral, 72 h, administration	47.87μg fipronil equivalent (>90% fipronil sulfone)/g wet weight	[93]
brain	10 mg/kg bw, oral, single dose	3.74μg/g	Suppl. Fig. 2
urine	5 or 10 mg/kg bw, oral, daily/2 weeks or single dose	0.023–0.026μg/mL	[47]
urine	5 or 10 mg/kg bw, oral, daily/2 weeks	0.024 or 0.032μg/mL	[104]
urine	4μg/kg bw, oral, 3X/week/90 days	0.009–4.27 ng/mL	[106]
hair	4μg/kg bw, oral, 3X/week/90 days	4.58–306 pg/mg	[106]
Human
plasma	no known fipronil exposure (96 plasma samples)	0.1–3.9 ng/mL; detected in 25% of the samples.	[104]
plasma	conducted on 159 workers from a factory manufacturing fipronil-containing veterinary products in France	mean: 7.79 ng/mL (range: 0.37–42.45 ng/mL) detected in 155 out of 159 workers.	[92]
plasma	6 cases of self-poisoning with Regent ^®	maximum fipronil + fipronil sulfone level measured was 3.74μg/mL	[109]
urine	no known fipronil exposure (84 urine samples)	undetectable	[104]

Aβ₄₂ production triggered by pyrazoles in N2a-APP695 cells requires β- and γ-secretase activity as demonstrated by the fact that it was strongly inhibited by β- (inhibitor IV) and γ-secretases (BMS 299897, DAPT) inhibitors and by a γ-secretase modulator (‘Torrey Pines’ compound, inducing an increase in Aβ₃₈ and a decrease in Aβ₄₂ and Aβ₄₀) [48, 49] (Fig. 4). Aβ₃₈ production was also strongly reduced following pyrazole treatment, while Aβ₄₀ levels were only modestly affected, as measured by ELISA (data not shown) and by mass spectrometry (Fig. 5). Increased Aβ₄₂ production triggered by pyrazoles was further confirmed in HEK293 cells stably expressing AβPPsw (data not shown) and neurons derived from human iPSCs (see below).

Fig.4

Extracellular Aβ₄₂ production induced by pyrazoles is inhibited by β-secretase inhibitor IV, γ-secretase inhibitors DAPT and BMS 299897, and γ-secretase modulator ‘Torrey Pines’. N2a-APP695 cells were exposed to 10μM β-secretase inhibitor IV, 2μM BMS 299897, 2μM DAPT or 10μM ‘Torrey Pines’ compound. 1.5 h later cells were exposed to 50μM (2, 5, 8, 14, 16, 18) or 25μM (3, 4, 6) pyrazoles or 50μM aftin-5. Extracellular Aβ₄₂ levels were measured after 18 h and are expressed as fold change, ±SE, of Aβ₄₂ level in pyrazole-treated cells over the Aβ₄₂ level of control, vehicle-treated cells. Representative of two independent experiments performed in triplicates. Errors bars represent SE of all six values.

Fig.5

Absolute quantification of Aβ_38, Aβ₄₀, and Aβ₄₂ using LC-MS/MS. Levels of the three Aβs were determined by mass spectrometry in supernatants of N2a-APP695 cells following 18 h treatment with DMSO, 100μM of pyrazoles 18, 20, or 21. Amyloid levels are expressed as percentage of levels in vehicle-treated cells (average ± SE of quadriplicate values; absolute values in control cell supernatants are indicated) and Aβ₄₂/Aβ₄₀ ratios are indicated in parentheses. Horizontal dotted line indicates basal Aβ levels versus the values in DMSO-treated cells.

Mass spectrometric quantification and profile analysis of pyrazole-induced Aβs

Aβ₃₈, Aβ₄₀, and Aβ₄₂ were quantified in N2a-AβPP695 cell culture supernatants using LC-MS/MS [50, 51]. Like aftins [35, 36] and triazines [38], pyrazoles induced a reduction in Aβ₃₈ levels, a slight increase in Aβ₄₀ levels and a strong increase in Aβ₄₂ levels (Fig. 5).

We next analyzed, by IP-MS [52], the profile of Aβs produced by N2a-AβPP695 exposed to fipronil (18), fipronil sulfone (20) and fipronil desulfinyl (21) (Fig. 6). Cell supernatants were collected and Aβs were immunoprecipitated and analyzed using MALDI-TOF/TOF [52] (Fig. 6). Exposure to pyrazoles decreased the production of Aβ_1-18, Aβ_1-19, Aβ_1-33, Aβ_1-37, and Aβ_1-38. Aβ_1-40 levels showed a modest increase. In contrast, the two highly neurotoxic [6 , 53–55] amyloid peptides Aβ_1-42 and Aβ_1-43, which were undetectable in supernatants of control cells, were strongly induced in cells treated with fipronil (18) and its two metabolites.

Fig.6

Pattern of Aβs produced by N2a-APP695 cells exposed to pyrazoles 18, 20, or 21. Cells were treated for 18 h with DMSO or 20μM of each pyrazole. Cell supernatants were collected and analyzed as described. Quantification of all Aβs in N2a-APP695 cells supernatants are presented as percentage of total amyloids. Note the decrease in peptides Aβ_1-19, Aβ_1-37, Aβ_1-38, the increase in Aβ_1-40 and the appearance of Aβ_1-42 and Aβ_1-43 in the supernatants of pyrazole-treated cells (these two peptides are undetectable in the supernatant of DMSO-treated cells).

Neurons differentiated from human iPSCs of AD patient and healthy control

We next tested the effects of the active pyrazoles and aftin-5 on neurons differentiated for 4 weeks from human iPSCs derived from a healthy individual (AβPP WT, wild-type) or from a patient with familial AD (AβPP K724N mutation) [56 –58] (Fig. 7). AβPP K724N neurons produced more Aβ₄₂ versus Aβ₄₀ compared to AβPP WT neurons. As observed with triazines [38], addition of any of the active pyrazoles or aftin-5 resulted in further increase in Aβ₄₂ production, in both AβPP WT and AβPP K724N neurons (Fig. 7).

Fig.7

Pyrazoles trigger enhanced production of Aβ₄₂ versus Aβ₄₀ in neurons differentiated from human induced pluripotent stem cells. Neurons were derived from iPSCs obtained from healthy donor (APP WT, white bars) or from an AD patient with APP K724N mutation (grey bars). They were exposed for 24 h to DMSO (control), 100μM aftin-5 or the nine pyrazoles. Cell supernatants were collected for extracellular Aβ₄₀ and Aβ₄₂ levels measurement by an ELISA assay. Levels are expressed as Aβ₄₂/Aβ₄₀ ratios ± SE of triplicate values. Horizontal dotted line indicates level for basal Aβ₄₂/Aβ₄₀ ratios.

Pyrazoles affect the specificity of APP-C99 cleavage and Aβ production in a cell-free γ-secretase activity assay

In order to assess whether the pyrazole-based compounds modulate Aβ production through a direct effect on the specific processing of APP-C99 by the γ-secretase complex, we tested fipronil (18), fipronil sulfone (20), and fipronil desulfinyl (21) in a previously described cell-free activity assay performed with highly purified γ-secretase protease and APP-C99 substrate (a recombinant APP C-terminal fragment expressed in Escherichia coli as a fusion protein consisting of a N-terminal Met for translation initiation, amino acids 597–695 of the 695-amino acid human isoform of APP, and a C-terminal Flag tag sequence) [59, 60]. More specifically, Aβs generated by purified γ-secretase in the presence of 100μM of the pyrazole compounds were analyzed by immunoprecipitation and MALDI-TOF (IP/MS), as previously described [60]. We found that all three pyrazole compounds increased the Aβ₄₂/Aβ₄₀ ratio when compared to the DMSO control (Fig. 8). Importantly, our analysis further revealed that fipronil sulfone (20), but not fipronil (18) or fipronil desulfinyl (21), drastically increased the Aβ₄₃/Aβ₄₀ ratio by ∼2-fold when compared to the DMSO control (Fig. 8). These results strongly support the increased Aβ₄₃ production seen exclusively with fipronil sulfone (20) in N2a-AβPP695 cells (Fig. 6). Altogether, we found that pyrazoles favor the production of the toxic Aβ₄₂ and/or Aβ₄₃ peptides, both of which contribute to the formation of amyloid plaques and the development of AD.

Fig.8

Mass spectrometric analysis of the Aβs generated in cell-free γ-secretase assays, in the presence of DMSO or fipronils 18, 20, or 21. A) The Aβs generated by highly purified γ-secretase in the presence of 100μM of fipronil (18), fipronil sulfone (20) or fipronil desulfinyl (21) or DMSO (vehicle) were pooled from triplicates of the activity assays, immunoprecipitated overnight with the anti-Aβ antibody 4G8 and protein G and analyzed by MALDI-TOF in a reflectron mode. The Aβs generated from the recombinant APP-C99 contain an N-terminal methionine, which results in a mass shift of +149m/z when compared to endogenous Aβ peptides. B) Note the increased Aβ₄₂/Aβ₄₀ ratio for fipronils 18, 20, and 21, and the increased Aβ₄₃/Aβ₄₀ ratio for fipronil sulfone (20).

Pyrazoles shift the cleavage pattern of another γ-secretase substrate, alcadeinα

Like AβPP, alcadeins (Alcs) are sequentially cleaved by secretases, first by α-secretase, leading to N- and C-terminal fragments, the latter being then cleaved by γ-secretase to an intracellular domain and the p3-Alcs peptide, in a way similar to AβPP [61, 62] (see Fig. 6A in [38]). HEK293 cells stably expressing full length alcadeinα were used to investigate the effects of pyrazoles on alcadein cleavage. Alcadeinα is first cleaved on the N-terminal side (two possible sites) followed by cleavage by γ-secretase leading to p3-Alcα35 and p3-Alcα 2N+35, the latter representing the major peptide in cultured cells. HEK293-alcadeinα cells were grown till 70% confluence and treated with 100μM pyrazoles for 48 h. The secreted p3-Alcα peptides were recovered by immunoprecipitation and analyzed by MALDI TOF/MS (Fig. 9A). Quantification of p3-Alcα peptides showed that, compared to the p3-Alcα peptide profile in vehicle treated cells, concentrations of the main alcadeinα peptide (p3-Alcα2N+35) and p3-Alcα2N+37 peptide remained stable. In contrast, both p3-Alcα2N+34 and p3-Alcα2N+36 concentrations dropped significantly while the level of p p3-Alcα2N+38 peptide concentration strongly increased (Fig. 9B). These results show that, like for AβPP, pyrazoles induce a shift in the cleavage pattern of Alcadeinα, another γ-secretase substrate. Similar effects were seen previously with aftin-5 and triazines [38]. These observations further suggest that pyrazoles are more likely to interact with γ-secretase than with their substrates.

Fig.9

Pyrazoles alter the cleavage pattern of Alcadeinα, leading to increased p3-Alcα38 production. A) Immunoprecipitation/mass spectrometry spectra of p3-Alcα peptides produced by HEK cells expressing full length Alcadeinα exposed to various pyrazoles or DMSO (control). Cells were treated for 48 h with 100μM of each reagent and p3-Alc peptides were analyzed by MALDI-TOF/MS. A) Representative profile for each product showing the the p3-Alcα2N+34, p3-Alcα2N+35, p3-Alcα2N+36, p3-Alcα2N+37 and p3-Alcα2N+38 peaks. B) Relative quantification of p3-Alcα peptides produced by cells exposed to DMSO (control), fipronil (18), fipronil sulfone (20) or fipronil desulfinyl (21). Levels of each peptide are presented as ratios of p3-Alcα2N+38 versus p3-Alcα2N+35 (average ± SE of triplicate values).

Proteomics study of fipronil’s effects

In order to understand the molecular mechanisms of action of pyrazoles, we decided to analyze protein expression of cells exposed to fipronil sulfone (20) and dipropetryn, two structurally unrelated products which, despite their unidentified targets, share the property of inducing Aβ₄₂ production. N2a-Abeta PP cells were exposed for 18 h to 20μM fipronil sulfone (20), 100μM dipropetryn or 0.1% DMSO (control) and were processed for proteomics analysis. Cell supernatant Aβ₄₂ levels were increased as expected (data not shown). A total of 8,027 proteins were identified in the multiplex experiment (data not shown). The expression of 1,634 and 1,638 proteins was modified, respectively, in fipronil sulfone (20)- and dipropetryn-treated cells compared to control, DMSO-treated cells (Fig. 10A). Among these, 261 proteins were shared by cells treated with both products, out of which 178 were upregulated or downregulated in the same direction for both products (Supplementary Table 4). DAVID analysis of these 178 proteins showed that they gather in mitochondrial pathways, cell cycle control, AD (Fig. 10B).

Fig.10

Proteomics and phosphoproteomics analysis of N2a-APP695 cells exposed to fipronil sulfone (20) and dipropetryn. A) N2a-APP695 cells were exposed for 18 h to 20μM fipronil sulfone (20), 100μM dipropetryn or DMSO. This led to increased extracellular Aβ42 expression (3.58 ± 0.05 and 7.87 ± 0.81 fold change for fipronil sulfone (20) and dipropetryn, respectively, compared to DMSO-treated cells). Up/downregulated proteins in fipronil sulfone- (1634) and dipropetryn- (1638) treated cells versus DMSO-treated cells were identified and compared. Among the 261 common proteins, 178 proteins (listed in Supplementary Table 1) were either upregulated by both treatments or downregulated by both treatments. B) DAVID analysis of the 178 proteins common to fipronil sulfone (20) and dipropetryn treatments. Data are selected with p-value < 0.01 and FDR < 0.05. FDR, False Discovery Rate.

Fipronil is metabolized to fipronil sulfone, a stable metabolite which accumulates in adipose tissue and brain

We next investigated the pharmacokinetics and biodistribution of fipronil (18) and its metabolites in mice (Fig. 11) and rats (Supplementary Figure 2). Mice first received a single oral dose of fipronil (18) and blood, brain and adipose tissue were collected at various times during 72 h (Fig. 11). Plasma pharmacokinetics showed rapid elimination of fipronil (18) and parallel appearance of fipronil sulfone (20) which remained stable during the 72 h time-course (Supplementary Figure 1). No fipronil desulfinyl (21) was detected. Similarly, fipronil (18) transiently appeared in brain and adipose tissues, while fipronil sulfone (20) accumulated to a stable level in both tissues (Fig. 11A, B). Fipronil sulfone (20) reached a 5-6-fold higher concentration in adipose tissue compared to brain. Given the metabolic stability of fipronil sulfone (20), we next ran a two months duration study following a single oral administration of fipronil (18) (Fig. 12A). Fipronil sulfone (20) levels were determined at various times up to 56 days after the single administration. The half-life of fipronil sulfone (20) was found to be 14 ± 3 days, 17 ± 2 days, and 26 ± 3 days in plasma, brain, and adipose tissue, respectively (Fig. 11A). We next administered fipronil (18) daily, 5 days/week, for 3 weeks and measured fipronil sulfone (20) in plasma, brain and adipose tissue (Fig. 12B). This repeated oral dosing allowed the maintenance of a stable level of fipronil sulfone (20) in plasma, brain and adipose tissue (Fig. 12B). Pharmacokinetics and biodistribution of fipronil (18) and fipronil sulfone (20) were next studied in rats during a 14 days period, following a single oral administration. Results confirmed rapid metabolism of fipronil (18) to fipronil sulfone (20) (Supplementary Figure 2A). Fipronil sulfone (20) was much more stable and showed extensive accumulation in adipose tissue (peak concentration 48 h after oral administration of fipronil (18)) followed by slow release and/or metabolism (Supplementary Figure 2B).

Fig.11

Short-term (72 hrs) time-course of fipronil (18) and fipronil sulfone (20) bioaccumulation in brain (A) and epididymal adipose tissue (B) following a single oral administration of fipronil (18) to mice. Fipronil (18) (10 mg/kg) was administrated by oral gavage at time 0. Animals were sacrificed at various times and plasma, epididymal adipose tissue and brain were collected. Fipronil (18) and fipronil sulfone (20) levels were quantified by LC-MS/MS. Concentrations are expressed as μg/brain or μg/epididymal adipose tissue.

Fig.12

Long-term (56 days) time-course of fipronil sulfone (20) production and accumulation following single (A) or repeated (B) oral administrations of fipronil (18) to mice. A) Fipronil (18) (10 mg/kg) was administrated by oral gavage on day 0. Animals were sacrificed at various times and plasma, epididymal adipose tissue and brain were collected. Fipronil sulfone (20) levels were quantified by LC-MS/MS. B) Fipronil (18) (10 mg/kg) was administrated by oral gavage 5 days /week for 3 weeks (times of administration are indicated by black dots). Animals were sacrificed at various times and plasma, epididymal adipose tissue and brain were collected. Fipronil sulfone (20) levels were quantified by LC-MS/MS. Concentrations are expressed as μg/brain, μg/epididymal adipose tissue or μg/mL plasma.

DISCUSSION

This study reports on the induction of Aβ₄₂/Aβ₄₃ production, and the increased ratio of long versus short Aβs, in various cell cultures and by highly purified γ-secretase following exposure to several pyrazole insecticides. These results support previous results obtained with various drugs (fenofibrate, celecoxib, indomethacin, isoprenoids) [63], DAPT under certain conditions [64, 65], steroids [66], ceramide analogs [67], the peroxynitrite donor SIN-1 [68], Zinc [60], aftins [35 –37] and several triazine herbicides [38]. Altogether these results have two main implications:

They support the idea that the HCE contains several compounds able to shift the cleavage of AβPP toward the production of long, toxic, aggregation-prone Aβs, such as Aβ₄₂/Aβ₄₃, those which are classically associated with AD in humans and numerous animal models of AD. These compounds, if proven to modify this balance in animals (as suggested by the first preliminary results obtained with aftin-4 [28] and celecoxib or FT-1 [63]) and in human, may contribute to the onset, development and/or acceleration of AD. We suggest that they be collectively named “Alzheimerogens”, by analogy with cancer-inducing carcinogens. Identification of such compounds in our environment appears to be a priority to develop a prevention strategy.

Some of these compounds, particularly the metabolically stable, brain-permeable molecules, constitute new pharmacological research tools to investigate the role of γ-secretase, its substrates and products, in the onset of AD, in cell lines and animal models. In this context, one of our objectives is, by analogy with MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced Parkinsonism, to develop a pesticide-induced animal model of AD, which would allow the study of AD onset in wild-type (WT) animals. This is exemplified by the orally available fipronil (18) which is rapidly metabolized to fipronil sulfone (20), a very stable compound which accumulates in adipose tissue, crosses the blood-brain barrier and reaches stable levels in the brain (Figs. 11 and 12; Supplementary Figure 2). We are currently running long-term exposure experiments with fipronil (18) administered orally in WT mice and Tg2576 mice (overexpressing isoform 695 of human AβPP with the Swedish mutation (KM670/671NL)).

Mechanism of action of fipronil and other pyrazoles

As also observed with aftins [36] and triazines [38], pyrazoles insecticides, including fipronil derivatives, show a structure/activity relationship (Supplementary Table 1) in their effects on Aβ production – some are active, others are inactive-, showing that pyrazole insecticides devoid of Aβ₄₂ induction properties can be developed. The limited number of pyrazoles and their wide structural diversity precludes a clear identification of key structural features interacting with the unknown target leading to Aβ₄₂ production. Unspecific, detergent, hydrophobic, membrane, or protein structure disrupting actions are unlikely to account for the effects of pyrazoles. Several arguments support an effect on γ-secretase and/or its micro-environment: 1) induction of Aβ production by pyrazoles is inhibited by γ-secretase inhibitors or modulators (Fig. 4); 2) pyrazoles also affect the cleavage of another substrate of γ-secretase, alcadeins (Fig. 9); and 3) pyrazoles modify the cleavage pattern of AβPP by highly purified γ-secretase.

Our cell-free γ-secretase activity assays performed with highly purified enzyme and substrate (Fig. 8) show that fipronil (18), Fipronil sulfone (20), and fipronil desulfinyl (21) clearly increased the Aβ₄₂/Aβ₄₀ ratio. Remarkably, Fipronil sulfone (20) additionally increased by ∼2-fold the Aβ₄₃/Aβ₄₀ ratio. At the molecular level, the γ-secretase complex cleaves the APP-C99 substrate within the transmembrane domain (TMD), first at the ɛ-site located at the intracellular interface, to generate two different C-terminal APP intracellular domains (AICDs) of 50 or 51 aa length [69]. To generate Aβs of different lengths, γ-secretase next cleaves the N-terminus of the TMD every α-helical turn (corresponding to 3-4 amino acids), starting from ɛ-sites, following two production lines: the AICD51/Aβ₄₈ pathway (Aβ₄₈⇒Aβ₄₅⇒Aβ₄₂⇒Aβ₃₈) and the AICD50/ Aβ₄₉ pathway (Aβ₄₉⇒Aβ₄₆⇒Aβ₄₃⇒Aβ₄₀) [69]. Consistent with a direct effect of pyrazoles on APP-C99 processing, our results support an altered Aβ₄₈ pathway leading to an increased Aβ₄₂ production. Surprisingly, our study further revealed that fipronil sulfone (20) unambiguously and drastically increases the Aβ₄₃/Aβ₄₀ ratio (Fig. 8). This observation is important since Aβ₄₃ is known to be highly amyloidogenic and a main species involved in the formation of senile plaques [6 , 70]. As of today, Aβ₄₃ is known to be a direct precursor of Aβ₄₀ [69]. Therefore, the increased Aβ₄₃ production in the cell-free assay suggests that fipronil sulfone (20) blocks the processing of APP-C99 at position 43 in the Aβ₄₉ pathway, an event which is likely associated with reduced Aβ₄₀ production.

Further quantitative experiments are required to explain the increased Aβ₄₂/Aβ₄₀ or Aβ₄₃/Aβ₄₀ ratios, caused either by increased Aβ₄₂ or Aβ₄₃ production, a reduced Aβ₄₀ production or both. In any case, an altered Aβ profile in the presence of pyrazole insecticides strongly suggests that the compounds directly affect the positioning of the AβPP substrate in the lipid-bilayer, as previously observed for compounds known as inverse γ-secretase modulators (iGSM) [64]. Similarly to what we observed with fipronil (18) and fipronil desulfinyl (21), the iGSM nonsteroidal anti-inflammatory drugs (NSAIDs) Fenofibrate and Celecoxib have indeed been reported to be Aβ₄₂-raising and Aβ₃₈-lowering compounds [63]. Interestingly, Celecoxib is a pyrazole. The pyrazole scaffold has in fact been used extensively in therapeutic drug development (review in [71]). In contrast to iGSMs, some NSAID-based γ-secretase modulators (GSM), including sulindac-sulfide, lower Aβ₄₂ and increase Aβ₃₈ productions [63]. GSMs are of great pharmaceutical interest and are currently under clinical evaluation for their ability to reduce the production of the amyloidogenic Aβ₄₂ and Aβ₄₃ peptides. At the same time the potential risk from Aβ₄₂ and Aβ₄₃ producing iGSMs needs careful evaluation since some of these compounds are FDA-approved and widely used as NSAIDs. At the molecular level, it has further been shown that residues 29–36 (GAIIGLMV) of the Aβ motif in APP-C99 located at the TMD N-terminus represent the binding site of both Aβ₄₂-lowering GSMs and Aβ₄₂-raising iGSMs [63]. This raises the question whether the same binding site could be involved in the pyrazole-induced Aβ₄₂/Aβ₄₀ and Aβ₄₃/Aβ₄₀ ratio increase. Interestingly, we recently found that zinc can drastically raise the Aβ₄₃/Aβ₄₀ ratio by influencing the positioning of the APP-C99 TMD through binding to Lysine 28, which is located at the surface of the membrane and is involved in anchoring APP-C99 in the lipid bilayer [69]. This observation further supports the possibility that Lys28 and more globally residues 29–36 in APP-C99 are involved in the pyrazole-induced Aβ₄₃/Aβ₄₀ increase. Finally, novel GSMs have recently been reported that directly bind on PS1 [72]. Binding of these compounds to PS1 induced a conformational change in the enzyme-substrate interaction that modulated the protease activity and consequently the Aβ profile. Thus, one cannot exclude that fipronil (18), fipronil sulfone (20), or fipronil desulfinyl (21) also modulate AβPP-processing by directly binding to the γ-secretase complex. Further experiments are needed to decipher the mode of action of these compounds (see Supplementary Figure 3 for working model).

Fipronil exposure and consequences

Fipronil has been developed a selective inhibitor of insect GABA-gated chloride channels (review in [44]). It is one of the most widely used pyrazole insecticides in agriculture (seed, culture, and crop treatment), in urban pest control (coackroaches, termites, wasps, flies, and ants management in and around buildings), and in veterinary applications (fleas and ticks control for pets and cattle). Fipronil and its metabolites are therefore widely found [73] in urban soil, dust, wastewater, and residential runoff [74], in rural soil, rivers, and atmosphere [46 , 76], but also in various tissues of pet, farmland, and wild-life animals [75 , 78] (only a very small number of references are cited). Although fipronil (18) is relatively resistant to degradation, it is mostly oxidized to fipronil sulfone (20), reduced to fipronil sulfide (22), photodegradated to fipronil desulfinyl (21), hydrolyzed to fipronil amide (23, 28). Many other metabolites have been described ([45–47 , 73], review in [39]). Although fipronil shows low affinity for mammalian GABA-gated chloride channels compared to those of insects, toxicity studies carried out with fipronil and its metabolites in various vertebrates [39 , 77] as well as studies performed with mammalian cells studies [79 –83] cast some doubts about the safety of fipronil to humans (see Supplementary Table 5). This concern is amplified by 1) the existence of numerous metabolites which are poorly characterized in terms of toxicity, 2) their long persistence in the environment (soil, water, sediment, and atmosphere), 3) their accumulation in tissues, especially adipose tissue (Figs. 11 and 12), 4) the potency of fipronil sulfone on mammalian GABA-receptor [84], and 5) the fact that fipronil and its metabolites essentially meet all druggability criteria of Lipinski’s rule of Five (Supplementary Table 6). In addition, fipronil (along with neonicotinoids) is a major worldwide concern for the survival of pollinators [85 –89]. In mammals, fipronil shows oxidative stress, neurotoxic, thyroid, endocrine, lung inflammation, blood pressure, and reproductive effects (review in [44]) at high doses. In humans, fipronil triggers mild nervous troubles as seen in acute intoxication cases [90, 91] and apparently impacted thyroid functions of workers following occupational exposure [92].

As shown in Figs. 11 and 12, fipronil (18) is rapidly metabolized to a very stable product, fipronil sulfone (20), which readily accumulates in fat tissue. Table 1 reviews plasma and tissue levels of fipronil sulfone (20) reached experimentally in mice and rat or accidentally in humans (our data + literature data). The highest plasma levels reached experimentally during these relatively short exposures (3.7μg/mL, i.e., 8.2μM) are not very far from those which induce Aβ₄₂ and Aβ₄₃ production. Adipose tissue and brain levels reached experimentally are probably higher. 72 h after a single oral administration of ¹⁴C-fipronil to rats, about 50% and 2% of the radioactivity (essentially fipronil sulfone) were found in adipose tissue and brain, respectively [93]. Fipronil sulfone is also the primary metabolite produced in human via hepatocyte cytochrome P-450 oxydation [90 , 92–95]. Fipronil sulfone was detected in about 25% of 96 human serum samples (0.1–39 ng/mL which corresponds to 0.22–86 nM) [45]. These samples were obtained from individuals with no known fipronil exposure. However, fipronil applied to the fur of pets is easily and rapidly transferred to humans [96, 97]. Fipronil and fipronil sulfone levels were measured in urine and blood samples of 159 workers in a factory manufacturing fipronil-containing veterinary products [92]: 33 and 155 workers had detectable serum fipronil and fipronil sulfone (0.37–42.45 ng/mL, i.e., 0.82–9.32 nM), respectively. Incidentally, fipronil has recently been shown to promote adipogenesis [98] and adiposity/obesity has been suggested as a risk factor for AD [99, 100]. Adipose tissue may thus act as a trap and storage tissue for lipophilic, Aβ₄₂/Aβ₄₃ inducers like fipronil, other pyrazoles, other pesticides. The persistence of fipronil sulfone, its accumulation in adipose tissue, and its ability to enter the brain raises concerns on its possible long-term effects on the central nervous system, in particular on its possible effects on the production of toxic, aggregation-prone amyloidogenic Aβ₄₂ and Aβ_43, as shown in this article.

In conclusion, we have added some pyrazole insecticides, especially the main metabolite of fipronil, in the growing list of HCE products which are able to alter the specificity of Aβ production. Our hypothesis is that these products may, on a long-term, cumulative and additive basis, possibly in parallel with microorganisms [101] and microorganisms-derived products, alter the Aβ production pattern sufficiently to lead to AD pathogenesis, and thus qualify as potential “Alzheimerogens”. Gradual accumulation in and chronic release from adipose tissue and transfer to the brain may contribute to the onset, development, and acceleration of sporadic AD.

Footnotes

ACKNOWLEDGMENTS

We are grateful to Laetitia Bailly (Laboratoire Cobra, University of Rouen) for purifying the two fipronil enantiomers. We are grateful to Jasna Padovan and Zeljko Javorscak (Fidelta) for the rat PK studies, to Rebecca L. McMahen, Mark J. Strynar for providing fipronil sulfone chloramine. This work was supported by ‘Fonds Unique Interministériel’ PHARMASEA/TRIAD projects, « Agence nationale de sécurité sanitaire, de l’alimentation, de l’environnement et du travail » (ANSES), Fondation “Jérôme Lejeune” and CRITT-Santé Bretagne /FEDER (LM). This research was also partly supported by an FP7-KBBE-2012 grant (BlueGenics) to LM. MC is CIFRE/ManRos Therapeutics PhD fellowship recipient. KB is a Torsten Söderberg Professor. HZ is a Wallenberg Academy Fellow. This work was supported in part by Grant-in-aid for Scientific Research 26293010 (TS) and 24790062 (SH) from the Ministry of Education, Culture, Sports, Science and Technology in Japan. HG, JF, and PCF were supported by the Fondation Eclosion. Partial support was provided by NIEH S/ROI ES002710, NIEH S Superfund Research Program P42 ES004699 and NIH/454 NS079202.

Authors’ disclosures available online ().

Although fipronil is not the most potent Aβ₄₂ inducer, it was selected for more detailed studies, among other pyrazoles, for several reasons: 1) its very wide use all over the world (despite its ban in Europe in America for food plant cultures), 2) its wide use against fleas and ticks in domestic animals, 3) its wide presence in urban and rural environment, 4) its chemical stability and environmental persistence (especially that of its metabolite, fipronil sulfone), 5) the biological activity of the main metabolite, 6) the fact fipronil sulfone accumulates in adipose tissue, crosses the blood-brain barrier and accumulates in the brain, and 8) its availability in large quantities for very cheap prices (in preparation of our current long term exposure experiments the supply issue needed to be solved). Incidentally, fipronil was selected before the public health scandal of summer 2017 in Europe (contamination of eggs by fipronil, presumably even more by fipronil sulfone)!

The supplementary material is available in the electronic version of this article: .

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Induction of Amyloid-β 42 Production by Fipronil and Other Pyrazole Insecticides

Abstract

Keywords

INTRODUCTION

MATERIAL AND METHODS

RESULTS

Screening HCE compounds reveals pyrazole insecticides as Aβ42 inducers

Mass spectrometric quantification and profile analysis of pyrazole-induced Aβs

Neurons differentiated from human iPSCs of AD patient and healthy control

Pyrazoles affect the specificity of APP-C99 cleavage and Aβ production in a cell-free γ-secretase activity assay

Pyrazoles shift the cleavage pattern of another γ-secretase substrate, alcadeinα

Proteomics study of fipronil’s effects

Fipronil is metabolized to fipronil sulfone, a stable metabolite which accumulates in adipose tissue and brain

DISCUSSION

Mechanism of action of fipronil and other pyrazoles

Fipronil exposure and consequences

Footnotes

ACKNOWLEDGMENTS

References

Screening HCE compounds reveals pyrazole insecticides as Aβ₄₂ inducers