The incidence of Acute Leukemia (AL) subtypes varies according to geographical distribution and more predominant in developing countries. The aim here was to evaluate the usefulness of cost effective diagnostic tools in characterization of Acute Lymphoblastic Leukemia (ALL) in resource poor population. One hundred and two AL cases were diagnosed. For diagnosis, cytochemical analysis and immunohistochemistry were performed. Among the children < 12 years, ALL was 64.3% while AML accounted for 30%. In patients > 12 years, ALL was 59.4% and AML was 31.3%. The B-ALL occurred most frequently than T-ALL in both the age groups while based on immunophenotyping in AML, CD13 was the most commonly expressed antigen. Hence, cost effective diagnostic tools namely the immunophenotyping and cytochemistry are useful and improve accuracy and rapidly risk-stratify patients that were diagnosed with acute leukemia.
AroraB, KurkureP and ParikhP. Childhood Cancers: Perspectives in India. Journal of Indian Medical Association. 2005; 103: 479-82.
2.
AroraRS, EdenT and KapoorG. Epidemiology of childhood cancer in India. Indian Journal of Cancer. 2009; 46: 264-271.
3.
BajelA, GeorgeB, MathewsV et al. Treatment of children with acute lymphoblastic leukemia in India using a BFM protocol. Paediatric Blood and Cancer. 2008; 5: 621-628.
BonillaM, MorenoN, MarinaN, DeReyesG, ShurtleffSA, DowningJR, BehmFG, HarrisonPL, RibeiroRC, PenaO et al. Acute lymphoblastic leukemia in a developing country: preliminary results of a nonrandomized clinical trial in El Salvador. J Pediatr Hematol Oncol. 2000; 22(6): 495-501.
6.
BoyleP, LevinB. Cancer control in low-resourceenvironment. World cancer report. IARC Press, Lyon 29-31, SEER 2007.
7.
ChiarettiS, ZiniG and BassanR. Diagnosis and Subclassification of Acute Lymphoblastic Leukemia. Mediterranean Journal of Hematology and Infectious Diseases. 2014; 6(1): e2014073.
DakkaN, BellaouiH, KhattabM, Brahimi-HornMC, AouedL, BouzidN, BakriY, BenjouadA. Immunologic profile and outcome of childhood acute lymphoblastic leukemia (ALL) in Morocco. J Pediatr Hematol Oncol. 2007; 29: 574-580.
10.
DattaK, ChoudhuriM, GuhaS and BiswasJ. Childhood Cancer Burden in Part of Eastern India-PopulationBased Cancer Registry Data for Kolkata (1997-2004). Asian Pacific Journal of Cancer Prevention. 2010; 11: 1283-1288.
11.
DaveyFR, ErberWN, GatterKC and MasonDY. Immunophenotyping of acute myeloid leukemia by immuno alkaline phosphatase (APAAP) labelling with a panel of antibodies. Am. J. Haematol. 1987; 26: 157-166.
12.
Den BoerML, KapaunP, PietersR, et al. Myeloid antigen co-expression in childhood acute lymphoblastic leukaemia: Relationship with in vitro drug resistance. Br J Haematol. 1999; 105(4): 876-82.
13.
El-SayedAM, El-BoraiMH, BahnassyAA, El-GerzawiSM. Flow cytometric immunophenotyping (FCI) of lymphoma: correlation with histopathology and immunohistochemistry. Diagn Pathol. 2008; 3: 43.
14.
HannIM, RichardsSM, EdenOB, HillFG. Analysis of the immunophenotype of children treated on the Medical Research Council United Kingdom acute lymphoblastic leukaemia trial XI (MRC UKALLXI). Medical Research Council Childhood Leukaemia Working Party. Leukemia. 1998; 12: 1249-1255.
15.
HowardSC, CampanaD, SmithEC et al. Development of a regional flow cytometry center for diagnosis of childhood leukemia in Central Amercia. Leukemia. 2005; 19: 323-325.
16.
JaffeES. Introduction to the WHO classification. Am J Surg Pathol. 1997; 21: 114-115.
17.
KhanAH, GuptaG, MehtaA, BashirS, SharmaS, RasoolM. Acute lymphoblastic leukemia in northern India. Indian Journal of Basic and Applied Medical Research. 2015; 4(2): 372-381.
18.
MaherJ. Clinical immunotherapy of B-Cell malignancy using CD19-targeted CART-Cells. Current Gene Therapy. 2014; 14(1): 35-43.
19.
MandakovaP, CamprV, KodetR. Correlation of results of flow cytometry and morphologic findings in the diagnosis of malignant B-cell lymphoma. Cas Lek Cesk. 2003; 142: 651-5.
20.
MargaretD. Acute Leukemias, Cancer Management. 14 (2011).
21.
MargolinJF, PoplackDG. Acute lymphoblastic leukemia. In PizzoP, PoplackD, eds. Principles and Practice in Pediatric Oncology. Philadelphia, Lippincott, Williams & Wilkins, 1997; 409-462.
22.
MoonH, HuhJ, ChoMS, ChiH, ChungWS. A case of CD45-, CD19- precursor B cell acute lymphoblastic leukemia with an a typical morphology. Korean J Lab Med. 2007; 27(4): 253-6.
23.
MukdaE, PintaraksK, KomvilaisakP and WiangnonS. Cytochemistry and Multi-color Flow Cytometric Immunophenotype for Diagnosis of Childhood Acute Leukemia. J Hematol Transfus Med. 2011; 21: 23-31.
24.
NoronhaiEP, MarinhoIHT et al. Immunophenotypic characterization of acute leukemia at a public oncology reference center in Maranhão, northeastern Brazil. Brazil Sao Paulo Med J. 2011; 129(6): 392-401.
25.
OliveiraMS, MatutesE, RaniS, MorillaR, CatovskyD. Early expression of MC S2 (CD13) in the cytoplasm of blast cells from acute myeloid leukaemia. Acta Haematology. 1988; 80(2): 61-4.
26.
PuttiMC, RondelliR, CocitoMG et al. Expression of myeloid markers lacks prognostic impact in children treated for acute lymphoblastic leukemia: Italian experience in AIEOP-ALL 88-91 studies. Blood. 1998; 92(3): 795-801.
27.
RajalekshmyKR, AbithaAR, AnurathaN and SagarTG. Time Trend In Frequency Of Occurrence Of Major Immunophenotype In Paediatric Acute Lymphoblastic Leukemia Cases As Experienced By Cancer Institute, Chennai, South India During The Period 1989-2009. Indian Journal Of Cancer. 2011; 48(3): 34-45.
28.
RegoMFN, PinheiroGS, MetzeK, Lorand-MetzeI. Acute leukemias in Piauí: Comparison with features observed in other regions of Brazil. Braz J Med Bio Res. 2003; 36(3): 331-7.
29.
SatyanarayanaL, AsthanaS and LabaniS. Childhood Cancer Incidence in India: A Review of Population-Based Cancer Registries. Indian Pediatrics. 2014; 51: 218-220.
