Short hairpin RNAs specific to human cytomegalovirus terminase subunit pUL89 prevent viral maturation

Mocarski E.S. , Liu A.C. , Spaete R.R. Structure and variability of the a sequence in the genome of human cytomegalovirus (Towne strain). J Gen Virol 1987; 68: 2223–2230.

Tamashiro J.C. , Spector D.H. Terminal structure and heterogeneity in human cytomegalovirus strain AD169. J Virol 1986; 59: 591–604.

Chou J. , Roizman B. The isomerization of the herpes simplex virus 1 genome: identification of the cis-acting and recombination sites within the domain of the a sequence. Cell 1985; 41: 803–811.

Black L.W. DNA packaging in dsDNA bacteriophages. In Calendar R. (Editor). The bacteriophages. New York: Plenum Press 1988; pp. 321–373.

Casjens S. , Huang W.M. Initiation of sequential packaging of bacteriophage P22 DNA. J Mol Biol 1982; 157: 287–298.

Bazinet C. , Benbasat J. , King J. , Carazo J.M. , Carrascosa J.L. Purification and organization of the gene 1 portal protein required for phage P22 DNA packaging. Biochemistry 1988; 27: 1849–1856.

Guasch A. , Pous J. , Ibarra B. Detailed architecture of a DNA translocating machine: the high-resolution structure of the bacteriophage Φ29 connector particle. J Mol Biol 2002; 315: 663–676.

Kochan J. , Carrascosa J.L. , Murialdo H. Bacteriophage lambda preconnectors. Purification and structure. J Mol Biol 1984; 174: 433–447.

Kocsis E. , Cerritelli M.E. , Trus B.L. , Cheng N. , Steven A.C. Improved methods for determination of rotational symmetries in macromolecules. Ultramicroscopy 1995; 60: 219–228.

Bogner E. Human cytomegalovirus terminase as a target for antiviral chemotherapy. Rev Med Virol 2002; 12: 115–127.

Bogner E. , Radsak K. , Stinski M. F. . The gene product of human cytomegalovirus open reading frame UL56 binds the pac motif and has specific nuclease activity. J Virol 1998; 72: 2259–2264.

Bogner E. , Reschke M. , Reis B. , Mockenhaupt T. , Radsak K. Identification of the gene product encoded by oRF UL56 of the human cytomegalovirus genome. Virology 1993; 196: 290–293.

Hwang J-S , Bogner E. ATPase activity of the terminase subunit pUL56 of human cytomegalovirus. J Biol Chem 2002; 277: 6943–6948.

Schefficzik H. , Savva C.G.W. , Holzenburg A. , Kolesnikova L. , Bogner E. The terminase subunits pUL56 and pUL89 of human cytomegalovirus are DNA-metabolizing proteins with toroidal structure. Nucleic Acids Res 2002; 30: 1695–1703.

Scholz B. , Rechter S. , Drach J.C. , Townsend L.B. , Bogner E. Identification of the ATP-binding site in the terminase subunit pUL56 of human cytomegalovirus. Nucleic Acids Res 2003; 31: 1426–1433.

Thoma C. , Borst E. , Messerle M. , Rieger M. , Hwang J-S , Bogner E. Identification of the interaction domain of the small terminase subunit pUL89 with the large subunit pUL56 of human cytomegalovirus. Biochemistry 2006; 45: 8855–8863.

Hammond S.M. , Bernstein E. , Beach D. , Hannon G.J. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 2000; 404: 293–296.

Hutvagner G. , McLachlan J. , Pasquinelli A.E. , Balint E. , Tuschl T. , Zamore P.D. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 2001; 293: 834–838.

Giesen K. , Radsak K. , Bogner E. The potential terminase subunit pUL56 of HCMV is translocated into the nucleus by its own NLS and interacts with importin α. J Gen Virol 2000; 81: 2231–2244.

Burns J.C. , Friedmann T. , Driever W. , Burrascano M. , Yee J.K. Vesicular stomatitis virus G glycoprotein peudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci U S A 1993; 90: 8033–8037.

Gelderblom H.R. , Hausmann E.H.S. , Özel M.A. , Pauli G. , Koch M.A. Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins. Virology 1987; 156: 171–176.

Hwang J-S , Kregler O. , Schilf R. Identification of acetylated, tetrahalogenated benzimidazole d-ribonucleotides with enhanced activity against human cytomegalovirus. J Virol 2007; 81: 11604–11611.

Hannon G.J. RNA interference. Nature 2002; 418: 244–251.

Tripp R.A. , Tompkins S.M. Therapeutic applications of RNAi for silencing virus replication. Methods Mol Biol 2009; 555: 43–61.

Stegmeier F. , Hu G. , Richles R.J. , Hannon G.J. , Elledge S.J. A lentiviral micoRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci U S A 2005; 102: 13212–13217.

Sui G. , Soohoo C. , Affar E.B. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A 2002; 99: 5515–5520.

Yu J-Y , DeRuiter S.L. , Turner D.L. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci U S A 2002; 99: 6047–6052.

Brummelkamp T.R. , Bernards R. , Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 2002; 296: 550–553.

Dittmer A. , Bogner E. Specific short hairpin RNA-mediated inhibiton of viral DNA packaging of human cytomegalovirus. FEBS Lett 2006; 580: 6132–6138.

McGeoch D.J. , Rixon F.J. , Davison A.J. Topics in herpesvirus genomics and evolution. Virus Res 2006; 117: 90–104.

Baines J.D. , Poon A.P.W. , Rovnak J. , Roizmann B. The UL15 gene of herpes simplex virus encodes two proteins and is required for cleavage of viral DNA. J Virol 1994; 68: 8118–8124.

Sheaffer A.K. , Newcomb W.W. , Gao M. Herpes simplex virua DNA cleavage packaging proteins associate with the procapsids prior to its maturation. J Virol 2001; 75: 687–698.

Wills E. , Scholtes L. , Baines J.D. Herpes simplex virus 1 DNA packaging proteins encoded by UL6, UL15, UL17, UL28 and UL33 are located on the external surface of the viral capsid. J Virol 2006; 80: 10894–10899.

Haasnoot J. , Westerhout E.M. , Berkhout B. RNA interference against viruses: strike and counterstrike. Nat Biotechnol 2007; 25: 1435–1443.

Boden D. , Push O. , Lee F. , Tucker L. , Ramratnam B. Human immunodeficiency virus type 1 escape from RNA interference. J Virol 2003; 77: 11531–11535.

Westerhout E.M. , Ooms M. , Vink M. , Das A.T. , Berkhout B. HIV-1 can escape from RNA interference by evolving an alternative structure in its RNA genome. Nucleic Acids Res 2005; 33: 796–804.

Liu Y.P. , Gruber J. , Haasnoot J. , Konstantinova P. , Berkhout B. RNAi-mediated inhibition of HIV-1 by targeting partially complementary viral sequences. Nucleic Acids Res 2009; 37: 6194–6204.

Champier G. , Hantz S. , Couvreux A. New functional domains of human cytomegalovirus pUL89 predicted by sequence analysis and three-dimensional modelling of the catalytic site DEXDc. Antivir Ther 2007; 12: 217–232.