Study on the Effects of Allitride and Its Mechanisms on Renal Cell Carcinoma in Vitro

Abstract

Objective

To study the killing effect of allitride on human renal cell carcinoma cell line Ketr-3 and its possible mechanisms.

Methods

The Ketr-3 cells were treated with allitride and the morphological changes were observed with inverted microscope. The cytotoxicity was estimated through theamine blue tetrazolium bromide (MTT). Apoptotic cells were detected by in situ cell apoptosis detection kit, and confirmed by flow cytometry. Changes of apoptosis rate cell cycle were assessed by flow cytometry. Caspase-3 (cysteine aspartate specific proteinase) mRNA was detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), and Caspase-3 protease activity was estimated with colorimetry

Results

MTT assay and morphological changes confirmed the killing effect of allitride on Ketr-3 cell line. FCM also showed that S-phase and G2/M-phase arrest was induced. RT-PCR and colorimetry confirmed that there was apparently a rise of Caspase-3 mRNA and Caspase-3 protease activity.

Conclusion

Allitride could kill Ketr-3 effectively by inducing apoptosis. Cell cycle arrest and up-regulation of Caspase-3 may play an important role in the mechanisms of killing effect.

Keywords

Renal cell carcinoma Apoptosis Allitride

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References

Guidelines on renal cell carcinoma. (Chinese urological association, 2009^th edition)

Leig

X.Y.

, Yao

S.Q.

Apoptosis induced by diallyl disulfide in human breast cancer cell line MCF-7.

Acta Pharmacol Sin. 2008 Oct; 29(10): 1233–9.

Xiao

, Zeng

Diallyl trisulfide selectively causes Bax-and Bak-mediated apoptosis in human lung cancer cells.

Environ Mol Mutagen. 2008 Sep 17.

Gayathri

, Gunadharini

D.N.

Effects of diallyl disulfide (DADS) on expression of apoptosis associated proteins in androgen independent human prostate cancer cells (PC-3).

Mol Cell Biochem. 2008 Aug 31.

Steinmetz

K.A.

, Kushi

L.H.

, Bostick

R.M.

, Folsom

A.R.

, and Potter

J.D.

, Vegetables, fruit, and cancer prevention: a review, J. Am. Diet Assoc. 1996; 96: 1027–1039.

Steinmetz

K.A.

, and Potter

J.D.

, Vegetables, fruit, and colon cancer in the Iowa's Women's Health Study, Am. J. Epidemiol. 1994; 139: 1–15.

C.C.

, Chung

J.G.

, Tsai

S.J.

Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells(J).

Food Chem Toxicol, 2004; 42(12): 1937–47.

Tsai

C.W.

, Yang

J.J.

, Chen

H.W.

Garlic organosulfur compounds up regulate the expression of the p i class of glutathione S-transferase in rat primary hepatocytes(J).

J Nutr, 2005; 135(11): 2560–5.

Lew

, Xiao

, Zeng

oligosulfide chain lengh and allyl groups are critical structural determinants for antipro-liferative activity of garlic-derived organosulfides against PC23 human prostate cancer cells (J).

Cancer Res, 2003; 44: 197.

10.

Xiao

, Choi

, Johnson

D.E.

Diallyl trisulfide-induced apoptosis in human prostate cancer cells involves c-Jun N-terminal kinase and extra-cellular-signal regulated kinase-mediated phosphorylation of Bcl-2 (J).

Oncogene, 2004; 23(33): 5594–606.

11.

Hosono

Takashi

, Fukao

Tomomi

, Ogihara

Jun

, and Ariga

Toyohiko

† Diallyl Trisulfide Suppresses the Proliferation and Induces Apoptosis of Human Colon Cancer Cells through Oxidative Modification of β-Tubulin. Chin Med J (Engl). 2007 Aug 5; 120(15): 1336–42.

12.

Kai-yuan Mao Xiao-peng

Cao

Apoptosis of BIU87 cells induced by allitridi, Journal of Sun Yat-sen University (Medical Sciences) 2005, 26, 4: 432–8.

13.

Xiao

Dong

, Lew

Karen L.

Diallyl Trisulfide Suppresses Growth of PC-3 Human Prostate Cancer Xenograft In vivo in Association with Bax and Bak Induction.

Clin Cancer Res. 2006 Nov 15; 12(22): 6836–43.

14.

De-ren

ZHANG

, Li-zu

XIAO

The pathological changes of spinal cord tissues of rabbits after intrathecal injection of collagenase.

Modern Rehabilitation, 2001, 5(8): 38–39.