The Relationship between CSE1L Expression and Axillary Lymph Node Metastasis in Breast Cancer

Abstract

Aim

CSE1L is the human homologue to the yeast gene CSE1 and CSE1L is a gene related to cancer progression. Thus, CSE1L may regulate the invasion and metastasis of breast cancer. The aim of this study is to show the relationship between CSE1L and axillary lymph node metastasis.

Methods

Sixty-six breast cancer patients were evaluated according to patient and tumor characteristics. Immunohistochemistry was carried out on formalin-fixed, paraffin-embedded archival breast tumor tissues. The results of CSE1L staining were analyzed according to the percentage of immunoreactive cells.

Results

There were 29 patients without axillary lymph node metastasis and 37 patients with nodal metastasis. The mean age of the patients was 50.6 ± 11.3 years. Age, tumor size, nuclear grade and hormone receptor status were similar in the axillary lymph node positive and negative groups. There was a statistically significant relationship between cytoplasmic CSE1L expression and axillary lymph node metastasis. However, nuclear CSE1L expression did not have any effect on axillary lymph node metastasis.

Conclusions

Cytoplasmic CSE1L overexpression may be a valuable tool for prognosis of breast cancer in future.

Keywords

Breast cancer CSE1L Lymph node Metastasis

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References

Brinkmann

Gallo

Pastan

Cloning and characterization of a cellular apoptosis susceptibility gene, the human homologue to the yeast chromosome segregation gene CSE1. Proc Natl Acad Sci USA. 1995; 92(22): 10427–10431.

Tanaka

Ohkubo

Tatsuno

Prives

hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell. 2007; 130(4): 638–650.

Rao

Gao

Huang

Mutations of p53 and K-ras correlate TF expression in human colorectal carcinomas: TF down-regulation as a marker of poor prognosis. Int J Colorectal Dis. 2011; 26(5): 593–601.

Tai

C.J.

Shen

S.C.

Lee

W.R.

Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells. Exp Cell Res. 2010; 316(17): 2969–2981.

Scherf

Pastan

Willingham

M.C.

Brinkmann

The human CAS protein which is homologous to the CSE1 yeast chromosome segregation gene product is associated with microtubules and mitotic spindle. Proc Natl Acad Sci USA. 1996; 93(7): 2670–2674.

Wellmann

Krenacs

Fest

Localization of the cell proliferation and apoptosis-associated CAS protein in lymphoid neoplasms. Am J Pathol. 1997; 150(1): 25–30.

Tai

C.J.

Hsu

C.H.

Shen

S.C.

Lee

W.R.

Jiang

M.C.

Cellular apoptosis susceptibility (CSE1L/CAS) protein in cancer metastasis and chemotherapeutic drug-induced apoptosis. J Exp Clin Cancer Res. 2010; 29(1): 110.

Behrens

Brinkmann

Wellmann

CSE1L/CAS: its role in proliferation and apoptosis. Apoptosis. 2003; 8(1): 39–44.

Behrens

Brinkmann

Fogt

Wernert

Wellmann

Implication of the proliferation and apoptosis associated CSE1L/CAS gene for breast cancer development. Anticancer Res. 2001; 21(4A): 2413–2417.

10.

Liao

C.F.

Luo

S.F.

Shen

T.Y.

CSE1L/CAS, a microtubule-associated protein, inhibits taxol (paclitaxel)-induced apoptosis but enhances cancer cell apoptosis induced by various chemotherapeutic drugs. BMB Rep. 2008; 41(3): 210–216.

11.

Böni

Wellmann

Man

Y.G.

Hofbauer

Brinkmann

Expression of the proliferation and apoptosis-associated CAS protein in benign and malignant cutaneous melanocyte lesions. Am J Dermatopathol. 1999; 21(2): 125–128.

12.

Liao

C.F.

Luo

S.F.

L.T.

Lin

C.Y.

Chen

Y.C.

Jiang

M.C.

CSE1L/CAS, the cellular apoptosis susceptibility protein, enhances invasion and metastasis but not proliferation of cancer cells. J Exp Clin Cancer Res. 2008; 27(1): 15.

13.

Tai

C.J.

T.C.

Jiang

M.C.

Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage. J Transi Med. 2013; 11(1): 29.

14.

Liao

C.F.

Lin

S.H.

Chen

H.C.

CSE1L, a novel microvesicle membrane protein, mediates Ras-triggered microvesicle generation and metastasis of tumor cells. Mol Med. 2012; 18: 1269–1280.

15.

Tung

M.C.

Tsai

C.S.

Tung

J.N.

Higher prevalence of secretory CSE1L/CAS in sera of patients with metastatic cancer. Cancer Epidemiol Biomarkers Prev. 2009; 18(5): 1570–1577.

16.

Brinkmann

Gallo

Polymeropoulos

M.H.

Pastan

The human CAS (cellular apoptosis susceptibility) gene mapping on chromosome 20q13 is amplified in BT474 breast cancer cells and part of aberrant chromosomes in breast and colon cancer cell lines. Genome Res. 1996; 6(3): 187–194.

17.

Wellmann

Flemming

Behrens

High expression of the proliferation and apoptosis associated CSE1L/CAS gene in hepatitis and liver neoplasms: correlation with tumor progression. Int J Mol Med. 2001; 7(5): 489–494.

18.

Brustmann

Expression of cellular apoptosis susceptibility protein in serous ovarian carcinoma: a clinicopathologic and immunohistochemical study. Gynecol Oncol. 2004; 92(1): 268–276.

19.

Peiró

Diebold

Baretton

G.B.

Kimmig

Löhrs

Cellular apoptosis susceptibility gene expression in endometrial carcinoma: correlation with Bcl-2, Bax, and caspase-3 expression and outcome. Int J Gynecol Pathol. 2001; 20(4): 359–367.

20.

Vinodkumar

Syamala

Abraham

E.K.

Balakrishnan

Ankathil

Germline BRCA1 mutation and survival analysis in familial breast cancer patients in Kerala; South India. J Exp Clin Cancer Res. 2007; 26(3): 329–336.

21.

Chen

S.L.

Iddings

D.M.

Scheri

R.P.

Bilchik

A.J.

Lymphatic mapping and sentinel node analysis: current concepts and applications. CA Cancer J Clin. 2006; 56(5): 292–309, quiz 316–317.

22.

Coskun

Dogan

Karaman

Ozaslan

Atalay

Value of sentinel lymph node biopsy in breast cancer patients with previous excisional biopsy. J Breast Cancer. 2012; 15(1): 87–90.