A study was conducted to establish the level of calcium and magnesium in human semen, seminal plasma and spermatozoa. One hundred and sixteen healthy, fertile young men submitted sample for this study. Total sperm count and active motility showed a positive correlation. Both elements were found more outside spermatozoa. They maintained a positive correlation. Spermatozoa count did not show correlation with any of these two elements. There was a negative correlation in calcium inside and outside spermatozoa.
JensenT.K., CarlsenE., JørgensenN.Poor semen quality may contribute to recent decline infertility rates.Hum Reprod.2002; 17: 1437–40.
2.
SackR.G.. Components of semen quality.J Ani Sci.1982; 55(Suppl): 1–13.
3.
OwenD.H., KatzD.F.. A Review of the physical and chemical properties of human semen and the formulation of a semen simulant.J Andrai.2005; 26: 459–69.
4.
MannT.. The biochemistry of semen and of the male reproductive tract.London, Methuen and Company Ltd, 1964.
5.
LiP., ZhongY., JiangX.Seminal plasma metals concentration with respect to semen quality.Biol Tr Element Res.2012; 148: 1–6.
6.
SkandhanK.P., AbrahamK.C.. Presence of several elements in seminal plasma of normal and of different pathological conditions.Andrai.1984; 16: 587–88.
7.
SkandhanK.P., PandyaC.B.. Direct couple plasma emission spectroscopic study on human seminal plasma.Adv Contr Dely Syst.1986; 2: 256–57.
8.
SkandhanK.P., MehtaY.B., CharyT.M.Semen electrolytes in normal and infertility cases. I. Sodium, potassium, calcium and magnesium.J Obstet Gynecol India.1978; 28: 278–85.
9.
ValsaJ., SkandhanK.P., GusaniP.H.Quality of 4-hourly ejaculates - levels of calcium and magnesium.Andrai.2013; 45: 10–17.
10.
ValsaJ., GusaniP.H., SkandhanK.P.Copper in daily and split ejaculates.J Reprod Med.1994; 39: 725–28.
11.
SkandhanK.P.. Importance of zinc in the semen quality of different fractions of split ejaculate.Infertility.1981; 4: 67–81.
12.
ValsaJ., SkandhanK.P., Sahab KhanP.Split ejaculation study: semen parameters and calcium and magnesium in seminal plasma.Cent Eur J Urol.2012; 65: 216–18.
13.
World Health Organization (WHO), Laboratory manual for the examination and processing of human semen, Geneva, 5th edition, 2010.
14.
EliassonR.. Super vital staining of human spermatozoa.Fertil Steril.1977; 28: 1257.
15.
Varley. Practical biochemistry,Great Britain: English Language Book Society, 4th edition, 1969.
16.
ValsaJ., SkandhanK.P., Sahab KhanP.Estimation of calcium and magnesium in seminal plasmas. A comparative study by colorimetry and atomic absorption spectrophotometry.Int J Analyt and Bioanalyt Chem.2013; 3: 23–26.
17.
BelseyM.A., EliassonR., GallegosAJ Eds. Laboratory manual for the examination of human semen and semen-cervical mucus interaction, Singapore, WHO, Geneva/Press concern; 1980.
18.
SkandhanK.P., MazumdarB.N.. Interdependence of count and motility of spermatozoa.Acta Eur Fertil.1983; 13: 49–54.
19.
TempletonA.A., MortimerT.. Is abnormal semen liquefaction associated with human male infertility?IRCS Med Sci.1981; 9: 72.
20.
AmelarR.D.. What to do when all fails.Fert Ster.1978; 29: 233.
21.
AmelarR.D., DublinL.. Semen analysis, In Male infertility,AmelarR.D., DublinL., WalshP.C. Eds. Philadelphia: WB Saunders Co; 1977.
22.
SkandhanK.P., JiyoC.S., AmithS.. Different electrolytes and metals in human seminal plasma.Gazzetta Medica Italiana.2007; 166: 181–86.
23.
ValsaJ., SkandhanK.P., AvniK.P.S.Calcium and magnesium in male reproductive system and its secretion. II. Within subject variability in human seminal plasma and spermatozoa.Urologia.2007; 75: 94–96.
24.
LindemannC.B., GoltzJ.S., KanousK.S.. Regulation of activation state and flagellar wave form in epididymal rat sperm: evidence for the involvement of both Ca2+ and cAMP.Cell Motil Cytoskeleton.1987; 8: 324–32.
25.
MortonB., Harrigan-LumJ., AlbagliL.The activation of motility in quiescent hamster sperm from the epididymis by calcium and cyclic nucleotides.Biochem Biophys Res Commun.1974; 56: 372–79.
26.
SorensenM.B., BergdahlI.A., HjollundN.H.I.Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility.Molecul Hum Reprod.1999; 5: 331–37.
27.
PetersonR.N., FreundM.. Relationship between motility and the transport and binding of divalent cations to the plasma membrane of human spermatozoa.Fertil Steril.1976; 27: 1301–07.
28.
YanagimachiR.. Mechanisms of fertilization in mammals, In Fertilization and Embryonic Development In Vitro,MastroianniL.Jr., BiggersJ. D. Eds. New York: Plenum Press, 1981; 88–82.
29.
YanagimachiR., UsuiN.. Calcium dependence of the acrosome reaction and activation of guinea pig spermatozoa.Exp Cell Res.1974; 89: 161–74.
30.
NikoobakhtM., AlooshM., HasaniM.. Seminal plasma magnesium and premature ejaculation: a case-control study.Urol J.2005; 2: 102–05.
31.
OmuA.E., Al-BaderA.A., DashtiH.Magnesium in human semen: possible role in premature ejaculation.Arch Andrai.2001; 46: 59–66.
32.
AdamopouloseD.A., DeliyiannisV.. Seminal plasma magnesium, calcium and inorganic phosphate concentration in normozoospermic and subfertile men.Andol.15: 648–54.
33.
HomonnaiZ.T., MatzkinH., FainmanN.The cation composition of the seminal plasma and prostatic fluid and its correlation to semen quality.Fertil Steril.1978; 29: 539–42.
34.
UmeyamaT., IshikawaH., TakeshimaH.A comparative study of seminal trace elements in fertile and infertile men.Fertil Steril.1986; 46: 494–99.
35.
TashJ.S., MeanA.R.. Regulation of protein phosphorylation and motility of sperm by cyclic adenosine monophosphate and calcium.Biol Reprod.1982; 26: 745–63.
36.
LornageJ., GuerinJ F., CzybaJ.C.Influence of cations and albumin on human spermatozoa.Arch Androl.1983; 10: 119–25.
37.
BondaniA., AspeitiaE., AznarR.Correlation between sperm motility and electrolyte composition of seminal fluid in normal and infertile men.Fertil Steril.1973; 24: 150–54.
38.
PapadimasJ., BontisJ., IkkosD.Seminal plasma zinc and magnesium in infertile men.Arch Androl.1983; 10: 261–68.
39.
WhiteI.G., QuinnP.J., MurdochR.N.. Studies of cation in semen, In Proceeding of 8th international conference of the international planned parenthood federation.HankinsonR.K.B., KleimanR.L., EiksteinP. Eds. Santiago, Chili, 1967 April 9–17.
40.
JonesH.P., LenzR.W., PalevitzB.A.Calmodulin localization in mammalian spermatozoa.Proc Natl Acad Sci U S.1980; 77: 2772–76.
41.
BradleyM.P., ForresterI.T.. A [Ca2+Mg2+] ATPase and active Ca2+ transport in the plasma membranes isolated from ram sperm flagella.Cell Calcium.1980; 1: 381–90.
42.
BradleyM.P., ForresterI.T.. A sodium-calcium exchange mechanism in plasma membrane vesicles isolated from ram sperm flagella.Fed Eur Biochem Lett.1980; 121: 15–18.
43.
ForresterI.T., BradleyM.P.. Identification of calmodulin-like activity in human seminal plasma.Biochem Biophys Res Commun.1980; 92: 994–1001.
44.
GaffuriS., MalettoS., PoggioA.Sterility. Determination and significance of the macro-and oligo-mineral elements in normal and pathological semen.Minerva Ginecol.1969; 21: 1339–59.
45.
EliassonR., LindholmerC.. Magnesium in seminal plasma.Invest Urol.1972; 9: 286–89.
46.
LindholmerC., EliassonR.. In vitro release and uptake of zinc and magnesium by human spermatozoa.Int J Fertil.1974; 19: 56–62.
47.
LindholmerC., EliassonR.. Zinc and magnesium in human spermatozoa from different fractions of split ejaculates.Int J Fertil.1974; 19: 45–48.
