DUS Characterization of Rice ( Oryza Sativa L.) Using Morphological Descriptors and Quality Parameters

Abstract

India has recently enacted Distinctiveness, Uniformity and Stability (DUS) characterization for rice. This paper focuses on DUS characterization using morphological descriptors for 21 rice varieties planted in a randomized block design during two kharif seasons (2007 and 2008). Data were recorded for 60 DUS descriptors (46 qualitative and 14 quantitative) following guidelines from the International Union for the Protection of New Varieties of Plants (UPOV) and the Protection of Plant Varieties and Farmer's Rights Authority (PPV & FRA). Data on quantitative traits were subjected to Combined Over Years Distinctness (COYD) and Combined Over Years Uniformity (COYU) analyses. The descriptors offering the most discrimination were time to 50% heading, decorticated grain shape, and the colour of lemma and palea. The cultivar Sathi, an improved landrace entry, was observed to be the most distinct, with the rarest morphological features. Eight of the 21 qualitative and 8 of the 14 quantitative traits exhibited uniformity as determined by UPOV-recommended levels. Twelve of the quantitative traits were relatively stable as judged by seasonal variation in Phenotypic Coefficient of Variation (PCV) and Genotypic Coefficient of Variation (GCV) values. Finally, the varieties were clustered following the eight grouping characteristics recommended by PPV & FRA. All the approaches clustered the improved landrace entities separately from the conventionally bred varieties.

Keywords

plant breeding clustering rice DUS characterization India

Get full access to this article

View all access options for this article.

References

Joshi

M.A.

Sarao

N.K.

Sharma

R.C.

Singh

Bharaj

T.S.

(2007), ‘Varietal characterization of rice (Oryza sativa L.) based on morphological descriptors’, Seed Research, Vol 35, No 2, pp 188–193.

Mani

S.C.

(2013), ‘Status paper on rice in Uttarakhand’, Rice Knowledge Management Portal (RKMP), Directorate of Rice Research, Rajendranagar, Hyderabad, website: http://www.rkmp.co.in/sites/default/files/ris/ricestatewise/Status%20Paper%20on%20Rice%20in%20Uttarakhand.pdf (accessed 12 May 2013).

Nethra

Prasad

Rajendra S.

Gowda

(2005), ‘Varietal characterization based on seed, seedling and plant morphological traits in rice (Oryza sativa L.) genotypes’, Mysore Journal of Agricultural Science, Vol 39, No 3, pp 362–367.

Prakash

(2007), ‘DUS test in rice’, in Proceedings of Training Program in Rice, DRR, Hyderabad, pp 84–93.

Protection of Plant Varieties and Farmer's Rights Authority (PPV&FRA) (2007), ‘Specific DUS test guidelines for twelve notified crops – rice (Oryza sativa L.)’, Plant Variety Journal of India, Vol 1, pp 151–169.

Rani

Shobha N.

Rao

Subba L.V.

Viraktamath

B.C.

(2006), ‘National guidelines for the conduct of tests for distinctness, uniformity and stability: Rice (Oryza sativa L) – Zero Draft’, Directorate of Rice Research, Rajendranagar, Hyderabad, Andhra Pradesh.

Singh

V.P.

(2007), ‘Quality of direct seeded rice cultivars’, Agricultural Science Digest, Vol 2, pp 79–82.

Suman

Shankar

V.G.

Rao

Subba L.V.

Sreedhar

(2005), ‘Variability, heritability and genetic advance in rice (Oryza sativa L.)’, Crop Research, Vol 30, No 2, pp 211–214.

Union Internationale pour la Protection des Obtentions Végétales (UPOV) (2004), ‘Rice (Oryza sativa L.) Guidelines for the conduct of tests for Distinctness, Uniformity and Stability’, in TG/16/8 (2004-03-31), pp 6–25, website: http://www.upov.int/edocs/tgdocs/en/tg016.pdf (accessed 16 June 2013).

10.

Union Internationale pour la Protection des Obtentions Végétales (UPOV) (2008a), ‘Examining distinctness’, in UPOV TGP/9/1, pp 1–36, website: http://www.upov.int/edocs/tgpdocs/en/tgp_9_1.pdf (accessed 17 June 2013).

11.

Union Internationale pour la Protection des Obtentions Végétales (UPOV) (2008b), ‘Examining uniformity’, in UPOV TGP/10/1, pp 1–14, website: http://www.upov.int/edocs/tgpdocs/en/tgp_10_1.pdf (accessed 17 June 2013).

12.

Union Internationale pour la Protection des Obtentions Végétales (UPOV) (2013), International Union for the Protection of New Varieties of Plants, WHAT IT IS, WHAT IT DOES, UPOV Publication No 437(EN), 5 January, website: http://www.upov.int/export/sites/upov/about/en/pdf/pub437.pdf (accessed 17 June 2013).

13.

Watson

(2004), ‘Using the DUS software’, in Workshop on Data Handling, 9–11 June, Beijing, UPOV/DATA/BEI/04/12, organized by UPOV in cooperation with the State Forestry Administration of China, the Ministry of Agriculture of China and the State Intellectual Property Office of China with financial assistance from the Ministry of Agriculture, Forestry and Fisheries of Japan, pp 2–19.

14.

Yan

W.G.

Rutger

J.N.

Bryant

R.J.

Bokelman

H.E.

Fjellstrom

R.G.

Chen

M.H.

Tai

T.H.

McClung

A.M.

(2007), ‘Development and evaluation of core subset of the USDA Rice Germplasm Collection’, Crop Science, Vol 47, pp 869–878.