Journal of Advanced Oral Research

To assess if milled cobalt chromium (Co-Cr) alloy offers significantly better marginal accuracy than cast Co-Cr alloy for screw-retained long-span dental implant framework.

A search PICO was formulated using suitable keywords and an electronic search was initiated. The databases of PubMed, Cochrane Library, Google Scholar and Embase were searched for related articles. Bibliographies of randomised control trials and reviews, identified in the electronic search, were analysed for studies published outside the electronically searched journals. Electronic search identified 26 studies. A total of 16 studies were eliminated after reading the abstracts. Out of the remaining 10 studies, 3 were eliminated based on the inclusion and exclusion criteria, and finally 7 studies were finalised for systematic review.

Data were extracted from the included studies and analysed. The obtained data were suitable for meta-analysis, which showed an overall effect size z = 4.97 (P < .001) at 95% CI showing a significant statistical difference between milled and cast Co-Cr frameworks.

Milled cobalt chromium frameworks are significantly more accurate than cast frameworks for long-span implant-supported frameworks used in dental prosthetic rehabilitation. More randomized controlled trials need to be conducted with a larger sample size to get a more authentic conclusion in a clinical scenario.

The current exploratory research establishes a correlation between the general unemployment rate due to COVID-19 and its effect on dental healthcare service utilization, workforce, and education attainments.

The conceptual model utilized in the study is the circular flow diagram explaining economy organization. The study is an exploratory research review. PRISMA guidelines are followed for the review of articles. The literature data for the current review study is obtained from Web of Science, Statista, Grey Literature like, the Federal Bank St. Louis, American Dental Association (ADA), Health Policy Institute, to justify the economic impact in the dental industry sector. The search terms employed to search for literature from the Web of Science database are “Dental” and “COVID-19.” Only research articles published in the past one year in English language are included as the final sample of literature review. Statista, Federal Bank, and ADA are utilized to take into consideration evolving economic impact data due to COVID-19.

Much less of research has been performed on the impact of COVID-19 on dental economics, and this study is one of the insights of projections about COVID-19 impact in the dental healthcare sector. The projected hardships of the economy during and post pandemic demand for timely measures in place. The dental regulating bodies must undertake those protocols to save the dental healthcare industry.

Unlike other healthcare sectors, the impact of COVID-19 will disproportionately affect the dental healthcare sector for several reasons. Given dental healthcare services aid in generating nation’s revenue like any other sector, it demands urgent actions from regulating authorities.

COVID-19 is a new illness and a big threat to global health. No specific antiviral agents are available for its treatment. The finest approach to manage this threat is to clean our hands properly. Washing hands under running water is a better way to stop the spread of infections than using a hand sanitizer. The aim of the study is to show that washing hands is efficacious than using a dab of alcohol-based hand sanitizer.

A search was performed using three databases, PubMed, Scopus, and Google Scholar published from 2010 to 2019. The keywords for the survey were “hand-washing,” “hand sanitizer,” and “effective.” After the relevant articles were found, the critical appraisal was made to select those that were suitable for the systematic review. The inclusion criteria of the study were surveys, clinical studies, no sample size restrictions, and only English papers. Any conference abstracts, case reports, and unpublished data were excluded from this study.

On the basis of the keywords, 22 relevant articles were found, and of those, 17 articles were selected for the systematic review.

This study evaluated that washing hands with soap and water are more practical and efficacious than using the sanitizer on greasy and soiled hands. An alcohol-based sanitizer, if used correctly and in appropriate volume, can disseminate certain type of micro-organisms.

To evaluate and compare the effect of curcumin (CUR) and Nano-curcumin (N-CUR) on human-derived mesenchymal stem cells (MSCs) in a dose-dependent manner.

An experimental study performed with putative MSCs from a total of five systemically healthy subjects with chronic periodontitis. These putative MSCs were isolated by cell culture and were further characterized and identified by colony-forming unit assay and immunocytochemical analysis using cell surface markers CD105, CD146, CD45 and CD73. The identified MSCs were treated with different doses of CUR and N-CUR, and compared with α-minimum essential medium (α-MEM) for its cell viability by performing MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay for 48 and 72 hr. The statistically analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test and Bonferroni’s post hoc test.

Compared to the α-MEM group, both CUR and N-CUR treated cells have shown significantly (P = .029) higher survival rate at lower concentration (0.1 and 0.5 µM/L), at 48 hr incubation. However, there was no statistically significant difference between the CUR and N-CUR groups on cell survival rate at both 48 and 72 hr incubation. When compared between the concentrations of the same group, significantly higher cell viability (P = .001) was observed at lower concentrations (0.1, 0.5 µM/L) in both test groups after incubation for 48 and 72 hr.

Both CUR and N-CUR have a dose-dependent effect on human derived MSCs survival when incubated for 48 hr, whereas N-CUR shows increased cell survival rate even at 72 hr of incubation. Although, the cautious use of CUR and N-CUR at higher concentrations is recommended.

To analyze the association between teeth brushing (TB) and interdental cleansing (IDC) frequency changes with gingival health status and the stage and grade of periodontitis.

This cross-sectional study consisted of clinical examination and questionnaires. The questionnaires include demographic information and oral hygiene (TB and IDC) behavior. More than 18-year-old participants who underwent a periodontal treatment were randomly included. 548 individuals were classified as gingival healthy, gingivitis, and periodontitis according to the 2017 classification system. The degree of periodontitis was based on stage and grade systems. The data were analyzed by using Kruskal–Wallis and Mann–Whitney U tests.

The highest TB frequency (twice daily) was the highest in patients with gingivitis (83.7%) followed by healthy patients (82.9%) and patients with stage I periodontitis (52.2%). Compared to those who performed IDC daily, 18.9% of patients were healthy and 8.1% had stage II periodontitis. There was a statistically significant association between the oral hygiene behavior and periodontal health status of participants. Statistically significant association was seen between the TB and IDC with stage-grade of periodontitis. When the TB and IDC scores of different grade groups were compared at the same stage level, no statistically significant difference was found.

This study showed that periodontal health status was positively correlated with TB and IDC frequency.

To investigate whether bacteria that play a major role in periodontal disease pathology and in the formation of dental plaque also affect the formation of dental calculus, which is a predisposing factor for the initiation and progression of periodontal diseases.

This was an in vitro study, and cultures of bacteria were obtained from the American Type Culture Collection and Department of Biology, Faculty of Science, Atatürk University. Young cultures of bacteria of Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), Streptococcus gordonii (S. gordonii), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Porphyromonas gingivalis (P. gingivalis), Fusobacterium nucleatum (F. nucleatum), and Corynebacterium matruchotii (C. matruchotii) were prepared in media containing their specific enriching factors. B2 solid, B4 solid, and B2 liquid media were used to determine active calcification, whereas the mineral salt basal (MSB) medium was used to observe passive calcification. Calcification in the media was measured under light microscopy and in MSB using a spectrophotometer and was recorded as the percent transmittance.

S. mutans, S. sanguinis, and S. gordonii showed calcification in the B2 medium. S. mutans, S. sanguinis, S. gordonii, and C. matruchotii demonstrated calcification in MSB. A. actinomycetemcomitans, P. gingivalis, and F. nucleatum did not show any calcification.

It was concluded that streptococci present in dental plaque take part in the formation of dental calculus, whereas periodontopathogens have no role in the formation of dental calculus.

The degree of vertical (intrafamilial) and horizontal (extrafamilial) transfer of Streptococcus mutans (S. mutans) to a child shows differences according to cultures. The wrong care habits may result in transfer of S. mutans. The aim of this study was to determine the vertical and horizontal transmission of S. mutans to a child and effective factors.

Mothers and kindergarten staff of 37 children aged between 4 and 5 years who initially started in two kindergartens (Group 1, n = 19; Group 2, n = 18) were included in this in vivo study according to 95% reliability and 80% theoretical power. Intraoral examination of mothers and children made according to the World Health Organization criteria (dmf-t/DMF-T) was done, following which mothers were asked to fill questionnaire forms including knowledge and attitudes of them about oral/dental health care. 1 mL saliva samples received from children/mothers/staff were examined microbiologically in order to isolate and quantify S. mutans. Arbitrarily primed polymerase chain reaction analysis was done to determine the genetic characteristics of S. mutans. All examinations were repeated to evaluate the horizontal transmission in the fifth month. Parametric tests (t-test and two-way analysis of variance) were employed for comparison of the variables that meet the normality assumption and nonparametric tests (Mann–Whitney) were also used.

It was observed that there is a statistically significant correlation between mother DMF-T values and mother–child S. mutans similarity level (P < .05). There is a statistically positive correlation between the habits of using a common fork, spoon, glass, and mother–child similarity level.

Vertical and horizontal transfers occurred in Turkish families in order to prevent the transfer of S. mutans; wrong baby care habits that may lead to the transfer of S. mutans, which is the main factor in case of tooth decay, should be avoided.

To analyze the remineralization efficacy of casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) and 8% arginine on artificial early enamel lesions on primary teeth enamel samples.

In this in vitro study, artificial enamel lesions were created in 120 primary teeth. Teeth were randomly divided into 5 groups: group C: CPP–ACP paste; group CF: CPP–ACPF paste with 900 ppm flour; group A: paste with 8% arginine; group K+: 500 ppm NaF as a positive control; and group K–: deionized water as a negative control. After 4 weeks of the remineralization process, the effect of remineralization on samples of agents used the microhardness, atomic force microscope, and scanning electron microscope analyses. Statistical analysis was done using Statistical Package for the Social Sciences (SPSS version 20.0, SPSS Inc., Chicago, IL, USA). ANOVA and Tukey Post-Hoc multiple comparison test were applied (p < .05).

Increasing percentage values of the microhardness at groups C, CF, and A were significantly better than group K– (p < .05), whereas there is no statistically significant difference with group K+ (p > .05). The average surface roughness values of groups C and CF were similar with group K+ in AFM analysis, whereas average surface roughness values of group A were significantly higher than K+ group.

The CPP–ACP, CPP–ACPF, and arginine are effective agents for remineralization of early childhood caries lesions.

To evaluate the shear bond strength (SBS) of 3 fissure sealants used alone and with ozone and laser pretreatment.

For the SBS test, the flattened enamel surfaces of 90 healthy third molars were randomly divided into nine groups (n  =  10 for each groups), based on different sealants (BeautiSealant, Fuji Triage, and Grandio Seal) and pretreatment conditions (no pretreatment, pretreatment with ozone, and pretreatment with neodymium-doped yttrium aluminum garnet [Nd:YAG laser]). Ozone was applied for 30 s in caries superficial mode, and Nd:YAG laser was applied for 60 s. After the pretreatment procedures, sealants were placed inside a mold on the enamel surface. SBS was measured with a universal testing machine. Data were analyzed using analysis of variance. Tukey’s multiple comparison tests were applied to determine the difference between the groups (α  = 0.05).

The lowest SBS was observed in the Fuji triage group. Nd:YAG laser and ozone did not affect the SBS of BeautiSealant. However, significant differences were determined in the Fuji triage and Grandio Seal groups after pretreatment procedures (P  < .05).

Ozone and laser can be employed to disinfect fissures before BeautiSealant application.

To compare the surface roughness, microtensile bond strength (µTBS), and flexural strength of polypropylene (PP) fibers reinforced glass ionomer cements (GICs).

A comparative in vitro study was designed to test the PP fiber reinforced GIC, which was formed when 0.5–1 mm length PP fibers were added into the powder of conventional GIC. Four groups were prepared (Group 1: control, Group 2: 1 wt% PP fiber, Group 3: 3 wt% PP fiber, and Group 4: 5 wt% PP fiber) to evaluate flexural strength, surface roughness values, and µTBS. A total of 10 samples with 25 × 2.5 × 5 mm dimensions were prepared for each group to test flexural strength. Disk-shaped specimens (n = 10) of 2 mm thickness and 10 mm diameter were used to test surface roughness. A total of 24 human primary molar teeth were used to evaluate µTBS, and 12 sticks were obtained for each group. The fractured surface analyses of samples from µTBS was performed using scanning electron microscope. The data obtained from the experiments were recorded and analyzed with one-way analyses of variance technique, and the normality was tested using the Shapiro–Wilk technique. A significance level of .05 was used.

In flexural strength tests, Group 3 (3 wt% PP fiber) showed significantly increased values (p < .05) when compared with other groups. Group 4 (5 wt% PP) showed significantly highest values in surface roughness tests (p < .05). No significant differences were seen between the groups (p > .05) according to µTBS results. More PP fibers were seen in fractured surfaces, when PP ratio increases.

It was observed that increased PP fiber percentage showed increased surface roughness, and 3 wt% PP fiber gave optimal values for fracture toughness. Incorporation of PP fiber to GIC does not affect the bonding to primary tooth dentine.

To compare and evaluate the implant stability quotient (ISQ) measurements by two different radio frequency analysis (RFA) machines, Osstell Mentor and Penguin^RFA.

Twenty bone-level implants (3.7 × 10 mm²) were placed on a bovine bone in this in vitro study. The ISQ measurements were performed with the Penguin^RFA and Osstell Mentor machines using the multipeg of the Penguin system. The measurements were repeated three times for each direction and an average ISQ value was calculated. The results were averaged (mean ± SD) and the intraclass correlation coefficient (ICC) was calculated to assess the relationship between the measurements.

The mean ISQ values for the Osstell and Penguin machines were 77.60 + 2.11 and 78.05 + 2.04, respectively. The ICC was 0.958. The ISQ values obtained from the Osstell and Penguin machines were significantly compatible (P < .05).

Both of the RFA devices provided similar and reliable ISQ measurements.

Polymethyl methacrylate is the common material used as a denture base. Ease of application, stability in the oral environment are its advantages; however, its mechanical properties should be enhanced. This study aimed to evaluate the effect of different ratios of polypropylene fiber (PPF) in addition to denture base materials on impact strength, water sorption, and cytotoxicity.

Heat-cure acrylic resin specimens were prepared according to the manufacturer’s instruction by adding PPFs of 6 mm length in different ratios (1, 3, 5, 10, and 20 wt%). In order to determine the impact strength, specimens were subjected to a Charpy impact test machine after being kept in distilled water at 37°C for 48 h. A span of 40 mm was adjusted and a 0.5 J pendulum was used. The fractured surface of specimens was also analyzed using a scanning electron microscope. In addition, mouse fibroblast cells and agar diffusion tests were used for cytotoxicity determination. The results were analyzed using the Kruskal–Wallis and the Mann–Whitney U tests for determining impact strength, and Kruskal–Wallis and Tukey’s range tests were performed for determining water sorption values (P = 0.05).

5 wt% PPF group exhibited the highest water sorption and impact strength values, and the difference was statistically significant (P < .05). On the other hand, no cytotoxic effects were determined in PPF added groups. Furthermore, increased fiber concentration caused less water sorption.

Addition of PPFs in acrylic resin increased the impact strength and decreased water sorption without any cytotoxic effects.

To evaluate the prevalence, diameter, localization of the posterior superior alveolar artery (PSAA) and the relationship between PSAA and the floor of the maxillary sinus and the thickness of the maxillary sinus lateral wall using cone-beam computed tomography (CBCT).

In this retrospective study, more than 500 CBCT images were retrieved. Out of them 156 CBCT images were related to the sinus floor elevation, among which 120 CBCT images were enrolled in the study based on the inclusion criteria, and 36 CBCT images were excluded. A total of 120 CBCT images of the PSSA from 60 patients comprising 32 (53.3%) men and 28 (46.7%) women were analyzed. Statistical data were analyzed using SPSS for Windows version 22. Descriptive statistics (mean, standard deviation, frequency) were used for the analyses.

The ages of the participants ranged between 26 and 76 years and the mean age was 54.48 ± 10.92 years. The PSAA was detected on the right side in 95% of the CBCT scans and on the left side in 85% of the maxillary sinuses. At the first detection point (FDP) and last detection point (LDP), the mean values of the distance between the inferior border of the PSAA and the floor of the maxillary sinus were 9.54 ± 4.27 mm and 7.91 ± 4.75 mm, respectively. The mean diameters of the PSAA at the FDP and LDP were 1.36 ± 0.46 mm and 1.12 ± 0.35 mm, respectively.

Detection of the characteristics of the PSAA by using CBCT is crucial for handling the sinus floor elevation procedures uneventfully.

The cancer stem cells (CSCs) are known to be responsible for drug resistance and cancer relapse in the treatment of cancer. Identification and isolation of CSCs and study of their properties will play a crucial role in developing an effective drug against these targets. The aim of the study was to isolate CSCs from primary cancer by the tumorspheres enrichment method, to confirm by indirect immunofluorescence and gene expression of stem cell markers by using real-time polymerase chain reaction (RT-PCR) technique.

In this in vitro study, we enriched oral CSCs through tumorsphere formation assay from seven primary cultures of OSCC patients with defined serum media. The expression and localization of the cell surface markers of CD133 and CD44 were tested by indirect immunofluorescence. Gene expression of stem cell markers such as CD44, CD133, Oct4, Sox2, and Nanog were quantified by RT-PCR technique. One-way analysis of variance was applied to analyze gene expression.

Tumorsphere formation has been used to isolate the CSCs from the OSCC tissue culture. Both CD133 and CD44 antibody confirmed the presence of CSCs through indirect immunofluorescence. In comparison to parental cell lines, the expression levels of CD133, CD44, Oct4, Sox2, and Nanog stem cell were significantly higher in CSC-enriched subpopulations.

The cost-effective spheroid enrichment and the indirect immunofluorescence methods are useful for the isolation of CSCs from the primary tumor.

To determine the prevalence of orofacial mesenchymal neoplasms and classify them using the WHO criteria while appraising its applicability.

This was a retrospective study involving a total sample of 192 entries diagnosed as mesenchymal tumors for the period 1990-2018 at the Oral Pathology Department, University College Hospital, Ibadan. The histopathology records were examined and clinicopathological data were obtained. Hematoxylin and Eosin slides of the cases were assessed to confirm the diagnosis. Tumors were classified using the WHO 2013 classification. Data were analyzed and presented using descriptive statistics.

Orofacial mesenchymal tumors constituted 7.9% of 2402 biopsies. There were 26 diagnoses in 161 tumors of the WHO classification. Male-to-female ratio was 1.1:1. Peak age group was in the third decade. Soft tissue tumors were 61.5%, while bone tumors were 38.5%. Benign tumors constituted 49.5%, while sarcomas and intermediate tumors were 45.8% and 4.7%, respectively. The mandibular region (29.7%) was the most commonly affected site. Adipocytic and osteogenic tumors were the most prevalent tumors in the soft tissue and osteoarticular series, respectively, representing 19.8% and 32.3% of all cases, while orofacial sarcomas constituted 3.6% of all lesions seen.

The findings in this study are in line with those of previous studies. However, the WHO classification may not be suited for the orofacial region. Crafting of a classification system for mesenchymal tumors of the orofacial region would thus be necessary.

To characterize the lip print pattern in a study population and to ascertain its potential for sex determination.

A cross-sectional study was conducted among 300 undergraduate students (144 males and 156 females). Simple random sampling was used. Lip prints were obtained with lipstick and were classified according to Suzuki and Tsuchihashi classification. Data were analyzed with the Statistical Package for Social Sciences (SPSS) version 22 (IBM Corp., Armonk, NY, USA). The prevalence of lip print patterns was presented as frequencies and percentages, and the test of significance of gender difference was done with chi-square at the 95 percent confidence interval.

No two lip print patterns were found to be the same. There was a significant difference in the lip print patterns of females and males. In females, the pattern with the highest frequency was type II (40.4%). This was followed by type I (36.5%), type III (13.5%), type V (7.1%), and type IV (2.6%). In males, the pattern with the highest frequency was type IV (28.5%). This was followed by type III (26.4%), types I and V (each 16.0%), and type II (13.2%). When both sexes were considered together, type II was the most frequent pattern.

Lip print is unique to individuals and it has potential for sex determination. The most prevalent patterns in females and males were type II and type IV, respectively.