Using the “Bi-Digital O-Ring Test Imaging Technique”, the author has been able to accurately localize meridians and acupuncture points that correspond to specific internal organs and has found that most general patterns of meridians and the number of acupuncture points on each of the meridians of specific internal organs of the 12 main internal organs described in the literature of ancient Chinese medicine, are more or less correct, with the exception of some variations and inaccuracies. Each meridian of specific internal organs was found to be connected to the organ representation area in the cerebral cortex of specific internal organs. The acupuncture point has an area and occupies 3-dimensional space. It has a circular or slightly oval boundary with diameter in the range of 3 mm to 2.7 cm, although 6–12 mm are the most common diameters in human adults, with the exception of the area outside the corners of the nailbeds of the fingers and toes. Using the “Bi-Digital O-Ring Test Molecular Identification Method”, the author also found that within the boundary of most acupuncture points and meridian lines (including Heart, Stomach, and Triple Burner) were high concentrations of neurotransmitters and hormones, including Acetylcholine, Methionine-Enkephalin, Beta-Endorphin, ACTH, Secretin, Cholecystokinin, Norepinephrine, Serotonin, and GABA. On all these meridian lines, in addition to the above neurotransmitters and hormones, Dopamine, Dynorphin 1-13, Prostaglandin E1( (PGE₁) and VIP were found, but ihe latter do not usually exist within the boundary of the acupuncture point with the exception of the center midline of the acupuncture point where the meridian line is situated. Serotonin, Norepinephrine, and Cholecystokinin appeared in either one of the above 2 patterns, depending on the individual. Usually, no significant amounts of these neurotransmitters and hormones were found at the surrounding area outside of meridian and acupuncture points. However, the essential amino acid L-Tryptophan (which is a precursor of Serotonin), was usually found outside of the boundary of the acupuncture point and the meridian but not within the boundary of the acupuncture point and the meridian. Wherever Serotonin appeared, L-Tryptophan disappeared significantly and when the Serotonin disappeared, L-Tryptophan reappeared. In addition to the above common neurotransmitters and hormones, the Heart meridian had additional Atrial Natriuretic Peptide in both the meridian and its acupuncture points. Similarly, the Stomach meridian had additional Gastrin in both the meridian and its acupuncture points. Likewise, the Triple Burner meridian had additional Testosterone ( in the male ) and Estrogen ( especially Estriol and Estradiol in the female).

When an acupuncture needle was inserted into the center of an acupuncture point on the Heart meridian, all of the above common neurotransmitters and hormones appeared outside of the acupuncture point in a circular area. Within 20-30 seconds after the insertion of the acupuncture needle, Atrial Natriuretic peptide (when acupuncture was given in the acupuncture points of die Heart meridian), Gastrin (when acupuncture was given in the acupuncture points of the Stomach meridian), and Testosterone or Estrogen ( especially Estriol & Estradiol ) ( when acupuncture was given on the acupuncture points of the Triple Burner meridian ) were found almost all over the body, but usually disappeared within 4 minutes. When the acupuncture needle was inserted anywhere in the acupuncture point, in addition to release of the organ specific hormone, Acetylcholine and Prostaglandin E₁(PGE₁) also appeared simultaneously all over the body, regardless of the meridian or the acupuncture point, and they disappeared from outside of the acupuncture point within 3 minutes, Acetylcholine reappearing within the entire area of the acupuncture point, and Prostaglandin E₁ reappearing only on the meridian line at the center of the acupuncture point.

Within 20 or 30 seconds after acupuncture, Methionine-Enkephalin was often found in an entire circular area up to a radius of about 6 cm to 9 cm from the center of the acupuncture point, with the exception of the entire circular area within the boundaries of the acupuncture point. Methionine-Enkephalin thus detected outside of the boundaries of the acupuncture point disappeared within 10 to 15 minutes after its appearance, while Beta-Endorphin, Dopamine, Serotonin, and GABA more often appeared within a radius of about 3 cm to 6 cm or sometimes with much greater distances and often remained for a much longer time (more than 24 hours ) and gradually disappeared.

When the spastic muscles or vaso-constricted arteries interacted with these neurotransmitters and hormones, the spasticity as well as the pain in the area often disappeared rapidly. Similar findings were found also with low frequency transcutaneous electrical stimulation (by inducing significant muscle contractions without creating pain), mechanical stimulation (including Shiatsu), soft laser radiation (by 2mW Helium-Neon Laser for less than 5 seconds) given on the acupuncture point. When Qi-Gong was given on an acupuncture point, the neurotransmitters and hormones extended in a doughnut-shaped circular area with slightly larger maximum radius. However, immediately after Qi Gong, the neurotransmitters and hormones appeared only in a “doughnut-shaped” outer area, without neurotransmitters and hormones in the area corresponding to the “hole of the doughnut” including the acupuncture point itself in the center of the hole, where no neurotransmitters and hormones were found with the exception of the center midline of the acupuncture point on the pathway of the meridian.