Nutrition and Metabolic Insights

Rather erroneously, eggs consumption is linked to increase in plasma cholesterol content and incidents of cardiovascular diseases, cancer, stroke, or diabetes. This misconception which is more pervasive particularly in sub-Saharan Africa, has led to very low patronage of eggs intake. In this study, egg consumption patterns, desired egg characteristics, and the extent to which the perception of eggs consumption as a health risk is entrenched among consumers in the Volta Region of Ghana, were examined.

The study used primary data for the analysis and the 2-stage sampling technique was employed. First, 5 districts (Keta, Ho, Krachi East, Nkwanta South and North Tongu) were selected and afterward, a sample was randomly selected from each of the district. A well designed and pretested questionnaires were administered to the respondents.

It was found that cheaper price and deep yellow yolk were the most persuasive parameters that motivate consumer purchase. The relationship between educational level and awareness on cholesterol types was significant. More than half of the respondents held the view that egg intake results in an increase in serum cholesterol and leads to the incidence of serious health problems.

This study proves the urgent need for a concerted national public education effort to raise awareness about the nutritional and health benefits of eggs intake. Success in such awareness creation will go a long way to greatly minimize acute malnutrition in the Ghana.

We previously showed that cocoa, a rich source of polyphenols improved the age-associated health and extended the lifespan in C. elegans when supplemented starting from L1 stage.

In this study, we aimed to find out the effects of timing of cocoa exposure on longevity improving effects and the mechanisms and pathways involved in lifespan extension in C. elegans.

The standard E. coli OP50 diet of wild type C. elegans was supplemented with cocoa powder starting from different larval stages (L1, L2, L3, and L4) till the death, from L1 to adult day 1 and from adult day 1 till the death. For mechanistic studies, different mutant strains of C. elegans were supplemented with cocoa starting from L1 stage till the death. Survival curves were plotted, and mean lifespan was reported.

Cocoa exposure starting from L1 stage till the death and till adult day 1 significantly extended the lifespan of worms. However, cocoa supplementation at other larval stages as well as at adulthood could not extend the lifespan, instead the lifespan was significantly reduced. Cocoa could not extend the lifespan of daf-16, daf-2, sir-2.1, and clk-1 mutants.

Early-start supplementation is essential for cocoa-mediated lifespan extension which is dependent on insulin/IGF-1 signaling pathway and mitochondrial respiration.

Hydrogen sulfide (H₂S) is a pivotal gasotransmitter networking with nitric oxide (NO) and carbon monoxide (CO) to regulate basic homeostatic functions. It is released by the alternative pathways of transulfuration by the enzymes Cystathionine Beta Synthase (CBS) and Cystathionine Gamma Lyase (CSE), and by Cysteine AminoTransferase (CAT)/ 3-Mercaptopyruvate Sulfur Transferase (3MPST). A non-enzymatic, intravascular release is also in place. We retrospectively investigated the possibility to modulate the endogenous H₂S release and signaling in humans by a dietary manipulation with supplemented micronutrients (L-cystine, Taurine and pyridoxal 5-phopsphate/P5P).

Patients referring for antiaging purposes underwent a 10-day supplementation. Blood was collected at baseline and after treatment and the metabolome was investigated by mass spectrometry to monitor the changes in the metabolites reporting on H₂S metabolism and related pathways.

Data were available from 6 middle aged subjects (2 women). Micronutrients increased 3-mercaptopyruvate (P = .03), reporting on the activity of CAT that provides the substrate for H₂S release within mitochondria by 3MPST, decreased lanthionine (P = .024), reporting the release of H₂S from CBS, and had no significant effect of H₂S release from CSE. This is compatible with a homeostatic balancing. We also recorded a strong increase of reporters of H₂S-induced pathways including 5-MethylTHF (P = .001) and SAME (P = .022), reporting on methylation capacity, and of BH4 (P = .021) and BH2 (P = .028) reporting on nitric oxide metabolism. These activations may be explained by the concomitant induction of non-enzymatic release of H₂S.

Although the current evidences are weak and will need to be confirmed, the effect of micronutrients was compatible with an increase of the H₂S endogenous release and signaling within the control of homeostatic mechanisms, further endorsing the role of feeding in health and disease. These effects might result in a H₂S boosting effect in case of defective activity of pathologic origin, which should be checked in duly designed clinical trials.

Several diet quality scores have been developed to evaluate the health benefits of individual diets such as Healthy Eating Index (HEI), Dietary Approach to Stop Hypertension (DASH), and Mediterranean diet score (Med). This study aims to determine the relationship between dominant dietary health scores with the risk of atherosclerosis in Iranian adults.

This case-control study was conducted on 323 patients with atherosclerosis and 334 individuals without atherosclerosis as control group. Food Frequency Questionnaire was used for obtaining dietary intakes; then HEI, DASH score, and Med score was calculated. Logistic regression models were used to calculate Odds Ratios (OR) and 95% Confidence intervals (CI) between quartiles of the HEI, DASH and Med and atherosclerosis risk.

The results showed that total scores for HEI, DASH, and Med in control group was higher than the atherosclerosis group. The results also indicated that higher adherence to HEI (OR: 0.43; CI: [0.24, 0.76], P-trend = .006), DASH (OR: 0.48; CI: [0.3, 0.78], P-trend = .003), and Mediterranean pattern (OR: 0.4; CI: [0.21, 0.76]) decreased odds ratio of atherosclerosis.

Our findings suggest that adherence to HEI, DASH, and Mediterranean diet might be associated with a lower risk of Atherosclerosis and can have a positive effect on general health and prevention of chronic diseases in people.

Iodine deficiency causes various health problems such as mental defects, goiter, reproductive damage, hypo and hyperthyroidism, stillbirth, abortion, congenital abnormalities, cretinism, mental retardation, muscle anomalies, and reduced work output. Although the adverse effects on health and socio-economic development are well known, they persisted as a public health problem worldwide. Salt iodization is recommended as a simple cost-effective method to prevent iodine deficiency disorders. This study aimed to determine the magnitude, trends, and determinants of iodized salt availability in the household in Ethiopia.

The current study used the Ethiopian Demographic and Health Surveys conducted from 2000 to 2016 with a total of 57 939 households. Descriptive statistics were performed on selected background characteristics to provide an overall picture of the sample after considering sample weights. To ensure the representativeness of the sample we applied a complex sample design considering household weights, primary sampling units, and the strata associated with it. The Cochran–Armitage test was performed to assess the trend of iodized salt availability in the household. Multivariate logistic regression was used to determine the association between the dependent variable and independent variables. A significance level of .05 was chosen for all analyses.

The magnitude of iodized salt availability in the household was 28.45% in 2000, 54.34% in 2005, 15.42% in 2011, and 89.28% in 2016. Iodized salt availability increased from 28.45% [95% CI: 27.69-29.21] in 2000 to 89.28% [95% CI: 88.79-89.75] in 2016. Despite the decline from 2005 to 2011 in the percentage of households with iodized salt, overall, there was a significant increment from 2000 to 2016 in Ethiopia (P-value <.001). There were differences in the status of salt iodization in the administrative region, wealth, family size, and ownership of radio or television.

Remarkable progress has been made in Ethiopia regarding iodized salt availability in recent years. Besides the current efforts to achieve universal salt iodization, future interventions should prioritize specific groups like those with lower socioeconomic status and geographic areas with lower availability of iodized salt in the household.

To assess serum Cu/Zn SOD (Superoxide Dismutase) concentration in autistic children and evaluate its possible relationship to GI Symptoms.

Serum from 50 autistic children (31 with chronic digestive disease (most with ileo-colonic lymphoid nodular hyperplasia (LNH) and inflammation of the colorectal, small bowel and/or stomach) and 19 autistic children without GI disease), and 29 non autistic controls (20 age matched non autistic children with no GI disease and 9 age matched non autistic children with GI disease) were tested for Cu/Zn SOD using ELISAs.

Serum Cu/Zn SOD levels of autistic children were significantly lower than all non autistic controls (p < 0.0001). Serum Cu/Zn SOD of autistic children with severe GI disease was significantly lower than autistic children with no GI disease (p < 0.0001), non autistic children without GI disease (<0.0001) and non autistic children with GI disease (p = 0.0003).

These results suggest an association between Cu/Zn SOD serum levels and autism, particularly autistic children with GI disease, and that the concentration of serum Cu/Zn SOD may be a useful biomarker for autistic children with severe GI disease.

The autonomic nervous system (ANS) is affected by several factors, including major nutrients. However, their effects on the ANS remains unclear. Most studies had several limitations. They focused on humans, therefore they had difficulties excluding factors other than the nutrients. Their observation periods were too short (<4 hours) to align with typical absorption times of carbohydrates versus fats. They assessed the effects compared with the pre-prandial state rather than comparisons between different nutrient types.

We aimed to investigate the effects of carbohydrates and fats on the ANS.

We employed a rat model to exclude the effects of external stimuli, used sufficient observation period, and compared the ANS parameters among animals fed 3 different diets. The rats were divided into carbohydrate-rich-diet (carb group) and fat-rich-diet (fat group) groups. We investigated the effects of carbohydrates and fats on the ANS by measuring heart rate variability parameters in rats. Electrodes and electrocardiography (ECG) transmitters were implanted in 14 Wistar rats maintained on a standard diet for 2 days followed by the experimental diets for 2 days. ECG readings were continuously recorded for 4 days. The R waves, function of the R-R interval, and time were calculated. A fast Fourier transform was used to obtain the power spectrum of the fluctuation (low frequency [LF: 0.2-0.75 Hz]; high frequency [HF: 0.75-3.0 Hz]; LF/HF ratio).

Compared with the standard-diet group, the carb group showed significantly increased HF activity, while the fat group showed a significantly increased LF/HF ratio.

The results reveal a link between macronutrients and ANS activity.

Early identification and treatment of non-alcoholic fatty liver disease (NAFLD) could reduce overall mortality. Anthropometric measurements offer a simple and cost-effective method to potentially improve early detection of NAFLD and prevent its complications. This study aims to estimate the prevalence of NAFLD using the fatty liver index (FLI) and evaluate the effectiveness of certain anthropometric measurements in predicting NAFLD as diagnosed by FLI.

A cross-sectional analytical study was conducted with 1264 Saudi population without Type 2 diabetes mellitus (T2DM) non-alcoholic individuals at primary health care centers (PHCCs) in Jeddah city. Measurements included triglycerides, gamma-glutamyl transferase (GGT), glycated hemoglobin (HbA1c), and fasting plasma glucose (FPG). Measurements for neck circumference (NC), and calculations for weight-to-height ratio (WHtR) and weight-to-hip ratio (WHpR), along with FLI, were performed. NAFLD was identified in individuals with an FLI ⩾60. The receiver operating characteristic (ROC) curve analysis was utilized to assess the accuracy of WHtR, WHpR, and NC in detecting NAFLD, with Youden’s index determining the optimal cutoff points for these 3 indices.

The prevalence of NAFLD was found to be 30.9%. Among the markers, WHtR emerged as the most significant in indicating NAFLD, achieving an area under the curve (AUC) of 0.916; whereas NC and WHpR exhibited identical AUC values of 0.783. WHtR demonstrated superior diagnostic efficacy for identifying elevated FLI, with gender-specific cutoff values established at >0.57 for females and >0.61 for males. In all 3 markers, females exhibited higher sensitivity, specificity, and negative predictive value (NPV) compared to males.

WHtR could serve as a useful tool in the initial clinical screening for NAFLD among Saudi population without T2DM to identify those who may benefit from more comprehensive testing. Further local studies are warranted to confirm the levels of accuracy and the calculated cutoffs.

Rheumatoid arthritis (RA) is an inflammatory disease that can result in bone erosion, lean mass lowering, and increase of fat mass without changes in body weight. The dietary consumption of polyunsaturated fatty acids (PUFAs) has been assessed in many studies due to their potential anti-inflammatory effect.

The aim of this research was to identify if dietary intake of PUFAs associates with bone mineral density (BMD) and limb structural changes in early rheumatoid arthritis (ERA) compared to a population-based control group. The study was conducted because previous results have been insufficient.

The study group consisted of 83 ERA patients and 321 control subjects. A dual-energy X-Ray absorptiometry (DXA) machine was used to measure hip, lumbar spine, and radius BMD, as well as arm and leg fat, lean, and bone mass. Dietary habits and inflammatory markers were assessed to evaluate the effects to BMD and limb structural changes.

In ERA subjects, higher dietary consumption of PUFAs was associated with a decrease in arm fat mass (b −28.17, P = .02) and possibly with higher lumbar BMD (b 0.008, P = .058). Limb bone and lean mass changes were not associated with dietary intake of PUFAs.

Balanced nutrition is essential. Consuming PUFAs could be beneficial in ERA preventing structural changes to hands, but additional research is needed.

Several isolated ingredients have been proposed to increase growth hormone (GH) release, including Chlorophytum borivilianum and Velvet bean. A combination of these two ingredients has been packaged within an investigational dietary supplement. It was the purpose of the present investigation to determine the impact of acute ingestion of this supplement on circulating GH in healthy, exercise-trained men.

Fifteen men ingested the dietary supplement on two different days, separated by one week. Blood was collected from subjects before ingestion of the supplement and at 20, 40, 60, 80, 100, and 120 minutes post ingestion. GH was analyzed in serum samples using an ELISA method. Values for GH for each subject, at each collection time, were averaged over both test days and used in the main analysis.

Serum GH increased over time, with higher values at 60 minutes (1.56 ± 0.65 ng mL^-1; P = 0.04; +767%), 80 minutes (1.76 ± 0.69 ng · mL^-1; P = 0.02; +878%), and 100 minutes (1.48 ± 0.62 ng · mL¹; P = 0.05; +722%) compared to pre ingestion (0.18 ± 0.04 ng · mL^-1). A great deal of subject variability existed in the area under the curve (AUC) for GH, with pooled values ranging from 0.49 to 61.2 ng · mL^-1 · 2 hr^-1.

Acute ingestion of an investigational dietary supplement containing the active ingredients Chlorophytum borivilianum and Velvet bean results in an increase in circulating GH in exercise-trained men. Additional placebo controlled investigations are needed to extend these findings. Moreover, studies are needed to determine if chronic use of such supplementation leads to favorable changes in health-related parameters associated with increased circulating GH.

We have previously isolated a novel lysosomal cysteine protease, cathepsin Y, which produces a kinin-potenciating octapeptide from rat plasma. The present study aimed to elucidate the influence of protein-restriction on cathepsin Y mRNA expression in tissues of growing rat.

Weaning rats were fed either 20% casein (normal-protein, NP) or 5% casein (low-protein, LP) diet for 12 weeks, from post-weaning period to maturity. Cathepsin Y mRNA expression in tissues was examined using RT-PCR and compared with that of cathepsin L, a general marker of muscle atrophy.

Body, liver and spleen weights and diet intake of LP were significantly lower than those of NP. Cathepsin Y expression in LP was increased in liver (3.70 fold vs. NP) and spleen (1.28 fold) at early periods of protein-restriction, whereas that of cathepsin L was increased in liver and pectoral muscle through all intake periods. However, significant differences were detected in neither cathepsin Y expression in pectoral muscle nor cathepsin L expression in spleen throughout intake periods.

A significant up-regulation of cathepsin Y was observed in spleen and liver, suggesting that it may associate with immunodepression in rats under protein-malnutrition during the early post-weaning period.