Abstract
Free Technical Information
Avid Nano
Report: BladeCell Disposable Cuvette for Improved DLS Productivity
A new report from Avid Nano demonstrates how its BladeCell disposable cuvette improves dynamic light scattering (DLS) sample preparation time by a factor of 8 compared with using traditional quartz cuvettes. The 5-μL BladeCell cuvette is precision molded in black polycarbonate and requires no sample preparation beyond centrifuging to reduce the possibility of dust contamination that can affect DLS measurements. To fill, 5 μL of sample is simply pipetted against the walls of the BladeCell liquid trap, where it is retained by surface tension. The prepared cuvette is placed into the temperature controlled sample holder of the Avid Nano W130i, where it can be analyzed by DLS in seconds. After analysis, the sample is recovered by dipping a pipette tip into the liquid trap and removing the contents. Disposal of the BladeCell eliminates the cleaning steps required with quartz cuvettes.
Technical Note: Detecting and Measuring Sub-Nanometer Particles and Molecules
The new technical note (1107-T4) describes how a very small molecule with a low molecular weight (caffeine, MW 194.2 g/mol) was accurately measured at 1.4% concentration in aqueous solution. The results using Avid Nano's W130i DLS demonstrate that the light scattering signal from the dissolved caffeine is sufficient to produce a good-quality correlogram with an intercept >0.2 and highly reproducible data with low polydispersity. The mean radius was consistently reported at about 0.48 nm and molecular weight estimate around 400 Da indicating concentration-dependent self-association into dimers, a known characteristic of caffeine in aqueous solutions.
Fluidigm
Customer Web Videos
Informational videos by customers are now on the Fluidigm Web site. The first customer web video from Dr. Paul Robson of the Genome Institute of Singapore reports on how he and fellow scientists have generated significant single-cell expression data for a detailed molecular understanding of mammalian development from fertilization to embryo implantation, a process known as the preimplantation period. The knowledge gained has a direct impact on clinical applications in the areas of regenerative medicine and assisted reproduction. Another video from Professor Mylene Yao of Stanford University reports on how her group's work using Fluidigm IFC technology to undertake single embryo gene expression has allowed them to gain a better understanding of early embryo development.
INTEGRA
Application Note: Automated Production of High-Quality Blood Agar
A new application note demonstrates how combining INTEGRA's MEDIAJET Petri dish filler with the DOSE-IT peristaltic pump provides an optimized automated solution for the production of high-quality blood agar. The note describes an automated protocol that minimizes the time that the blood is exposed to hot agar medium and thus reduces thermally induced lysis of erythrocytes. By preparing the blood agar using an INTEGRA MEDIACLAVE media preparator, the dispensing temperature can be set and maintained at low temperature. By combining the MEDIAJET Petri Dish filler together with a DOSE-IT peristaltic pump and a special tubing set for blood agar, the experimenters are able to add blood to the agar immediately before pouring the plate thereby reducing blood cell lysis significantly.
More: www.integra-biosciences.com/pdf/liquid_handling/Blood_agar_preparation_V00.pdf
Integrated DNA Technologies
Quarterly Newsletter: Decoded
IDT now offers an educational newsletter that features regular sections such as “Your Research,” where readers can learn about research applications and findings plus sections dedicated to explaining fundamental scientific principles, tips, and ideas to streamline research, “Ask Alex” and a light-hearted science-humor feature.
More: www.idtdna.com/decoded
Oxford Gene Technology
White Paper: Next-Generation Techniques for Genomic Research
This new white paper by OGT discusses the relative merits of DNA microarrays and next-generation sequencing for human genome analysis. It discusses the essential factors to consider when selecting the optimal platform for a specific study, along with issues such as the suitability of microarrays and sequencing for various applications, the relative costs associated with each technology and how to overcome the challenges posed by the analysis of large and complex data sets.
SP Scientific
Technical Article: Impact of Product Resistance on Primary Freeze Drying
Authored by Dr. Margit Gieseler of GILYOS and Dr. Henning Gieseler of the Freeze Drying Focus Group at the University of Erlangen, this new technical article discusses how product resistance (Rp) has been recently suggested as a critical product parameter (CPtP) in freeze drying because it instantaneously draws a picture of the (already dried) inner cake morphology at the point of measurement. According to SP Scientific, from a physical point of view, Rp of the dried product layer impacts the resistance to water vapor flow and thus indirectly determines the maximally allowable shelf temperature and primary drying time. In addition to providing an introduction to Rp behavior, the article summarizes the results of trials investigating the Rp of sucrose based on different experimental conditions and using the Auto-MTM feature of the SP Scientific SMART Freeze Dryer software. The authors conclude resistance of the product to vapor flow during primary drying is a CPtP that impacts several process parameters. Lack of information about product performance during primary drying can lead to suboptimal product quality and/or problems during scale-up. The use of MTM allows an assessment of Rp data.
More: www.spscientific.com
Thermo Fisher Scientific
Thermo Scientific HyperSep SPE Column Applications Notebook
Highlighting the importance of sample preparation within the analytical laboratory, the HyperSep SPE column range provides a resource for the purification of a wide range of analytes. Containing information on the full list of HyperSep columns available, this 144-page benchtop resource provides technical specifications and method information to assist separations scientists in selecting the appropriate phase for their needs. Conveniently divided into pharmaceutical, environmental, forensic, and food safety applications, this resource also outlines workflow and selection processes to increase efficiency and assist with decision making.
TTP LabTech
Application Note: The Use of Acumen eX3 for the Identification of the Roles of Protein Kinase C α & δ Isoforms in PMA-Induced Ectodomain Shedding
A new application note from TTP LabTech illustrates how the company's Acumen eX3 laser scanning imaging cytometer can be used to elucidate novel cellular pathways involved in the shedding of the epidermal growth factor receptor (EGFR). The note describes how the high-throughput imaging technology of the Acumen eX3 quantifies and monitors protein expression levels.
According to TTP LabTech, online image capture and analysis, and multiplexing capabilities, allows the Acumen eX3 platform to provide consistent and accurate data from complex biological systems. The shedding of HB-EGF is classically controlled by a disintegrin and metalloproteinase, ADAM17. The imaging technology of Acumen eX3 is used to determine the contributions of additional enzymatic pathways in this process. HT1080 cells, transfected with a modified, alkaline phosphatase-linked, heparin binding EGFR (AP-HB-EGF) are examined by both the traditional enzymatic assay and immunofluorescence for receptor shedding in the presence and absence of inhibitors of ADAM17 and protein kinase C (PKC) isoforms. Experimental data suggest that important contributions are made by the PKC isoforms α & δ. Disruption of EGFR homeostasis is associated with a number of important cancer targets.
More: www.ttplabtech.com/products/acumen/acumen-bibliography.html
Application Note: Mosquito Crystal for Identifying the Structure of Intracellular Glutathione Transport Protein
Entitled “mosquito Crystal for the Rapid Screening and Optimization of Crystallization Conditions of the Bacterial Periplasmic Binding Protein, HbpA and the Elucidation of its Role as a Glutathione Import Protein,” this new application note describes how the mosquito Crystal was used to dispense 100-nL crystallization droplets (50-nL protein sample and 50-nL crystallization condition). This allowed rapid and efficient screening of almost 2000 crystallization conditions, ultimately resulting in the successful crystallization and identification of a novel GSH import protein.
More: http://www.ttplabtech.com/products/mosquito/bibliography.html
Application Areas
AMSBIO
ec23 Stable Synthetic Retinoid
According to AMSBIO, its new ec23 Stable Synthetic Retinoid has been proven to potently induce and reliably control stem-cell differentiation. ec23 enables the robust and reproducible differentiation of stem cells and progenitor cells. With higher stability in tissue culture medium and more potency as a differentiation factor than retinoic acid, ec23 produces downregulation of markers associated with the pluripotent stem-cell phenotype and increased expression of differentiation markers. The differentiation of neural tissues is clearly observed and additionally ec23 has been noted to trigger digit duplication during targeted release in the chick embryo, underlining its potency as a regulator of tissue development. As a potent inducer of neurogenesis, ec23 can maximize the consistency and efficiency of neural stem-cell differentiation and opens up a wealth of applications in basic research screening, toxicological testing, and disease modeling.
The AMSBIO Heparin/GAG Binding Plate and Arrays
According to AMSBIO, products for investigating the protein-binding properties of heparin, heparan sulfate (HS), and other glycosaminoglycans (GAGS) have traditionally been constrained by the need for chemical modification before attachment to inert or derivatized surfaces. The new AMSBIO Heparin/GAG Binding Plate offers a solution to this problem. The specially prepared plate surface adsorbs GAGS without modification while maintaining their protein-binding characteristics. Applications include elucidation of optimum GAG sequences and sulfation patterns for binding to proteins of interest, analyses of sulfotransferases and endosulfatases, and detection of inhibitors or enhancers of protein-GAG recognition. The new plate also can be used for studying receptor binding to growth factors or chemokines bound to surface-immobilized GAGS. The ELISA-type format of the Heparin/GAG Binding Plate uses protein reagents economically and is compatible with automated laboratory systems.
AMSBIO also has introduced new high-performance arrays designed for use with the Heparin/GAG Binding Plate. AMSBIO GAG Arrays can be used to gain information on the importance of the heparin and dermatan backbone structure and the position and density of sulfate groups for protein binding. The Heparin Oligosaccharide Array can determine the length of oligosaccharide required for optimum binding to a protein of interest. The Heparin/HS Array can find which of AMSBIO's naturally occurring heparins and HSs is most effective at binding proteins of interest. AMSBIO's K5 Polysaccharide Arrays offer a set of GAGs that contain novel sulfation motifs on N-acetylated or N-sulfated backbones.
More: www.amsbio.com
STEM-CELLBANKER
This new chemically defined freezing medium offers an optimized formulation for stem cells, iPS cells, and other valuable cells storage. Supplied ready to use with a simple usage protocol, STEM-CELLBANKER is completely free of serum and animal-derived components, and contains only European- or U.S. Pharmacopoeia-graded ingredients.
Part of the CELLBANKER series of cell-freezing media, STEM-CELLBANKER increases cell viability while maintaining cell pluripotency, normal karyotype, and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage. Independent research demonstrates that hESC, MSCs, and iPS cells cryopreserved with STEM-CELLBANKER produce higher cell viability (>90%) over conventional freezing medium, while retaining cell pluripotency, normal karyotype, and proliferation ability.
Every batch of STEM-CELLBANKER cryopreservation medium is performance tested on Jurkat and SK-007 cells. Additional standard evaluations for endotoxins, pH, osmolarity, and Mycoplasma contaminants are undertaken to ensure GMP equivalent quality.
More: www.amsbio.com/STEM-CELLBANKER-Stem-Cell-Cryopreservation-Solution.aspx
Bio-Rad Laboratories
ProteOn HTG Sensor Chip and Reagent Kit
For use with the Bio-Rad ProteOn XPR36 system, the new ProteOn HTG Sensor Chip's surface chemistry has increased binding stability and specificity for His-tagged proteins compared with chips from other surface plasmon resonance (SPR) system providers. Additionally, the HTG Sensor Chip's ability to regenerate and reuse HTG chips multiple times can reduce the cost of generating data. The ProteOn HTG Sensor Chip's Tris-NTA complex contains three NTA molecules for improved binding stability and selectivity to His-tagged molecules. This results in better data quality and reduced ligand decay. Furthermore, binding via polyhistidine tag is reversible, so the ligand can be removed from the surface and the chip can be reused 10 times or more. This increases reproducibility by eliminating chip-to-chip variability, and it decreases cost per data point.
To optimize the polyhistidine-tagged protein interaction workflow, Bio-Rad has also introduced the ProteOn HTG Reagent Kit for use with the ProteOn HTG Sensor Chip. The kit includes NiSO4 for chip activation and an EDTA solution for chip regeneration.
GE Healthcare
Whatman Indicating FTA DMPK Cards
For the collection of colorless biosample fluids, such as plasma, urine, and CSF, in pharmacokinetic and toxicokinetic studies, these new cards enable room temperature storage of dried biosample spots and require a low-sample volume (10–20 μL). The new cards also eliminate the need to freeze thaw.
ForteBio
Dip and Read Anti-Mouse IgG Fc Capture Biosensor for the Octet Platform
According to ForteBio, this new biosensor enables high-throughput rapid kinetic characterization and screening of mouse antibodies and other Fc-containing proteins with ease of use and cost efficiency. It eliminates steps typically associated with ELISA and SPR methods for screening and kinetic characterization, and facilitates high-throughput research applications such as hybridoma screening and characterization. The new anti-mouse IgG Fc capture (AMC) biosensor enables immobilization of mouse Fc-containing proteins on the biosensor directly, from a crude or purified matrix, without the need to biotinylate. Additionally, AMC biosensors can be regenerated and reused up to 10 times.
More: www.fortebio.com
Qiagen
Multiplex PCR Plus Kit
This new kit is designed for rapid and sensitive multiplex PCR that allows users to amplify several targets in one PCR run. The kit provides fast reaction times and comes with a preoptimized protocol.
The master mix included with the Multiplex PCR Plus Kit contains the enzyme HotStarTaq Plus DNA Polymerase, and a balanced combination of salts and additives, which increases multiplex reaction specificity and ensures comparable efficiencies for all primers in the PCR reaction. This proprietary technology allows straightforward assay development for many challenging applications, such as detection of mutations or microsatellites and microbial genotyping. The kit also includes CoralLoad—a buffer that contains two dyes for improved pipetting visibility during reaction setup, gel loading, and visualization of DNA migration.
Thermo Fisher Scientific
Accucore HPLC Columns
Founded on core-enhanced technology and using phase bonding and packing, these new columns are available in a wide range of stationary phase selectivities and compatible with almost any instrument. They offer increased sensitivity and peak resolution and provide a chromatography solution to enhance laboratory workflow and efficiency.
Containing solid core particles, which are engineered to a diameter of 2.6 μm and an average particle size distribution (D90/10) of 1.12, the Accucore HPLC column range allows high-speed, high-resolution separation, with backpressures significantly lower than those associated with UHPLC. The bonding technology results in excellent peak shapes through high-bonding coverage and minimized secondary interactions.
AssayAssure Sample Collection, Transport, and Storage Products
AssayAssure is a long-acting universal preservative that is compatible with a broad range of cellular, bacterial, and viral targets. It uses a chemical formulation to protect sample components from cell death and molecular degradation without refrigeration. Considered ideal for collecting a range of environmental, food safety, forensic, and biomedical swab samples, AssayAssure can also be used with containers for urine, molecular urine, and microbial samples. Protecting even sensitive RNA and DNA targets, these kits are suitable for PCR, qPCR, and cellular (flow cytometry, microscopy, and bacterial culture) applications.
TraceGOLD GC Metal Columns
This new range of gas chromatography (GC) columns are designed for higher temperature ranges than standard GC columns and can process highly active samples and analytes without incurring any damage. They are optimized for use in high-throughput analyses because they are resistant to the column breakdown and bleed that is often caused by repeated sample runs. TraceGOLD GC Metal Columns are available in three stationary phase formats: TG-1MT, TG-5MT, and TG-WAXMT, with a range of column lengths, internal diameters, and film thicknesses. According to Thermo, TraceGOLD GC Metal Columns are optimized to enhance analytical workflows, particularly for petrochemical, environmental, forensic science, and food safety applications in which high-sample volume or activity are common.
Bioservative
This new sample preservation matrix does not require refrigeration, can ensure the cell viability of microbiological samples for up to 7 days and delays the breakdown of RNA, DNA, and peptides within a sample for up to 26 days. Bacterial cells taken from the sampling environment and stored in Bioservative are held in a state of suspended metabolic activity, stopping continued growth and allowing accurate analysis of the bacterial content. The matrix also stabilizes the sample pH, preventing cell death and pH hydrolysis of biological molecules. Preservation of samples in this way protects against freeze-thaw damage, enabling convenient aliquoting from batch materials in long-term storage.
Ventana Medical Systems
OptiView DAB IHC Detection Kit
This fully automated immunohistochemistry (IHC) detection kit offers customizable staining, and according to Ventana, OptiView Detection enhances stain quality, allowing IHC assays to be viewed more clearly. The combination of enhanced chemistry and flexible software leads to quicker turnaround time for slides and allows more flexibility for labs to customize assays to their visible preferences and performance characteristics.
More: www.ventana.com
Automated Systems
Biohit
Roboline
This small, quiet, and compact liquid-handling automate offers open programmability and a fast drag-and-drop function to automate a single task or an entire application. Roboline can pipette any combination of columns, rows, single wells, or entire plates, and the closed environment protects reagents and samples from any contamination. The fully electronic air displacement pipetting operation produces error-free results, and is capable of forward pipetting, reverse pipetting, multiple dispensing, and diluting volumes from 5 to 200 μL using liquid-level sensing for error-free, small-volume pipetting.
In addition, the Roboline can reduce repetitive strain injuries and decreases exposure to harmful or toxic solutions.
More: www.biohit.com/roboline
Biotage
Initiator+ Microwave Synthesizer
According to Biotage, this new system has simple and intuitive software, an integrated computer control system and the largest touch-screen interface available. It allows users to drive reactions to as high as 300 °C and up to 30 bar pressure and offers an option for the use of a fiber-optic temperature measurement device for in situ reaction monitoring. The expanded pressure range of up to 30 bar allows for increased flexibility in the choice of solvents. Low boiling point solvents can be used at higher temperatures and reactions performed at maximum speed to achieve best possible yield with higher pressure. The Initiator+ can connect the SP Wave peptide synthesis module and the Robot Eight kit to perform microwave-assisted peptide synthesis with vortex mixing.
More: www.biotage.com
Initiator+ SP Wave
The Initiator+ SP Wave is a semiautomated, programmable synthesis system. It includes a large 10″ touch-screen, intuitive software, pre- or self-defined methods, inert gas capability, vortex, and magnetic stirring. It can be used as a stand-alone peptide synthesizer or converted to a high specification MAOS system. According to Biotage, it is considered an ideal development tool for synthesizing peptides, peptidomimetics, and small molecules using single- or multistep procedures. It is also considered well suited for the small-scale synthesis of peptides and peptidomimetics, including PNA using microwave irradiation, from a scale as low as 5 μmol. The semiautomated operation can reduce the consumption and waste of reagents.
More: www.biotage.com/peptides
Cytocentrics
CytoPatch Instrument
According to Cytocentrics, this fully automated device for gigaseal patch clamp measurements in the whole-cell configuration offers a true-patch pipette that resembles the manual patch clamp, a perfusion system that enables the measurements of very fast activating and desensitizing ligand-gated ion channels, and system scalability to meet different throughput demands.
The CytoPatch Instrument is the first automated patch-clamp platform in which the patch-clamp process takes place inside a three-dimensional (3D) quartz glass microchip with integrated microfluidic channels. This chip technology in combination with the CytoPatch perfusion system is considered best suited for measuring the inhibitory effect of slowly acting compounds (permanent application of 10 min possible) or the very fast activating and desensitizing ligand-gated ion channels (compound application time of <10 ms).
Each instrument is equipped with its own patch-clamp amplifier and advanced perfusion system along with its own cell, buffer, and compound supply. One CytoPatch Instrument can perform either as a stand-alone device or as a multiple-unit system in which up to 20 individual instruments can be connected on a network. This modular built-up makes it possible to run different assays in parallel with different cell types. The CytoPatch comes with well-established ion channel assays such as hERG, NaV1.5, KV1.5, and KV1.3.
More: www.cytocentrics.com
FEI Company
Versa 3D DualBeam System
The new Versa 3D's configurable platform provides high-resolution, 3D imaging and analysis on a wide range of sample types. Versa 3D is available with either high vacuum only or high- and low-vacuum electron imaging hardware. Low-vacuum electron-imaging capabilities allow the system to accommodate contaminating or outgassing samples that are incompatible with high-vacuum operation. Low vacuum also provides the ability to compensate for charge build up in nonconductive samples even at the high currents required for analysis techniques, such as energy-dispersive (X-ray) spectroscopy and electron backscatter diffraction. The platform also can be configured with low-vacuum capabilities and environmental scanning electron microscopy for in situ analysis. Advanced SEM scanning and FIB patterning yield powerful imaging and milling performance. New features, such as FEI's SmartSCAN and Drift Corrected Frame Integration, facilitate electron beam imaging of sample types with a range of different properties. Advanced backscattered electron, and secondary electron and ion detectors, collect a wide variety of topographic, elemental, and compositional information from every angle.
Titan G2 80–200 with ChemiSTEM Technology
According to FEI, this new member of the Titan G2 series of S/TEM (scanning/transmission electron microscopes) can deliver atomic resolution elemental maps in minutes and adds new capabilities for applications in materials science, chemistry, and nanotechnology. This new microscope includes features of the Titan G2 series, including the X-FEG high brightness gun and the next-generation DCOR probe corrector, achieving spatial resolution of 0.8 Å in STEM and 0.9 Å in TEM, while still maintaining a large, more flexible working space in the sample area, enabling applications requiring high-specimen tilt angles. For example, this large tilt range, when combined with ChemiSTEM Technology's symetrically distributed 4-SDD detector architecture permits EDX Tomography. Thus, 3D elemental composition can be obtained with standard FIB-prepared lamella samples mounted in standard holders in an S/TEM.
More: www.fei.com/chemistem
Horizon Technology
SmartPrep Extractor
SmartPrep is an automated, small footprint, extraction system designed for scalable cartridge-based SPE of a wide range of contaminants in clean aqueous matrices. The system enables the application of unique methods to each sample or process up to (96) samples in batch mode and is compatible with most 1-, 3-, and 6-mL cartridges and can process. The SmartPrep is modular and scalable. Eight extractors can be linked to one PC. SmartPreps software allows for individual method selection for each sample or batch processing. Ideal sample volumes are 10 mL–1.0 L.
More: www.horizontectinc.com
Olympus
VS120 Virtual Microscopy Slide Scanning System
This new system offers image deconvolution/deblurring for ultra-sharp images and provides new fluorescence imaging capabilities. Virtual slide creation can be achieved by setting the slide in place and pressing Start. According to Olympus, the system creates outstanding bright field slide scans up to three times faster. The VS120 can scan up to 100 slides at a time at very high fidelity, so that users anywhere can view and fully navigate high-resolution images of entire microscope slides or slide areas using a computer. It comes equipped with a 5-megapixel CCD bright field camera that improves bright field scanning speeds while delivering superb color reproduction and ultra-high resolution.
Acquiring sharp images at multiple depths using six or more fluorescence labels is possible, thanks to Olympus Fluorescence Virtual-Z capability. The software adjusts for the potentially different focal positions of various wavelengths in multichannel acquisition. Automated batch scanning can be accommodated with fluorescence samples and individual shading correction is available for each color channel. In addition, the VS120 provides for online deblurring during fluorescence acquisition, which improves image sharpness in real time. In addition to its faster scan speeds, the system offers more than 30 software enhancements. No-neighbor and nearest-neighbor deconvolution are available offline and a wide range of measurement and annotation functions are supported. Images can now be saved in .tiff and .btf file formats, along with the .vsi virtual slide file format. To support users working simultaneously with images from the Nanozoomer virtual microscopy scanning system, the .ndpi file format is supported.
Syrris
Asia 220 Flow Chemistry System
For chemical synthesis on the mg to kg scale, this system incorporates a wide range of temperatures (−15 to +250 °C), pressures, and flow rates, and can be used with solid- or solution-phase chemistry. According to Syrris, its high chemical compatibility enables a broad range of reactions. The Asia 220 can carry out a single reaction from start to finish, providing “walk-away” automation, without the need for a PC.
The Asia 220 enables automated synthesis. Users enter the reactor volume, desired temperature and pressure, collection volume and desired ratio of the reagents, then press start. The system automatically sets the flow rates, injects the correct volume of reagents, controls reaction temperature and pressure, diverts the waste, and starts and stops the collection when the reaction product reaches the Product Collector.
More: www.syrris.com
TAP Biosystems
CompacT SC Automated T-Flask Cell-Culture System
According to TAP, its new CompacT SC can be used to aseptically culture multiple different stem cell lines in parallel using T75 and T175 flasks, has a number of features that make controlling the growth and differentiation of stem cells easy. The system includes automated cell imaging and analysis that allows the CompacT SC to be programmed to monitor cell morphology and growth curves. It also permits the system to bet set up to respond to any preprogrammed changes by automatically passaging cells and adding new media, reagents, or vectors at appropriate times. This means it can maintain cell lines in the state needed, either as pluripotent cells or during cell differentiation and transfection. Processes can be performed at anytime of the day or night, without users having to be in the laboratory.
The CompacT SC also comes with a Low Volume Reagent Dispensing Option and an Incubator Oxygen Control Option to enable optimal conditions for stem cells by automatically setting the system to pipette chilled growth factors or transfection reagents at low volumes, and provide reduced oxygen levels to mimic in tissue conditions.
More: www.tapbiosystems.com
Uniqsis
Gas Addition Module for FlowSyn
According to Uniqsis, this new module enables fast, controllable presaturation of liquid reagents with a wide range of gases and so promotes efficient gas-dependent reactions in flow, such as hydrogenation, ozonolysis, carbonylation, and direct synthesis of carboxylic acids. The pressurized tube-in-tube design of the Gas Addition Module overcomes the problem of undissolved gas bubbles by ensuring continuous interaction between the gas and liquid at every point along its length. The tube-in-tube design is based on semipermeable membrane technology, whereby the semipermeable inner tube containing the liquid (typically a solvent) is bathed by a stream of pressurized gas that is enclosed within a thick-walled impermeable outer tube. The pressurized gas is able to cross the semipermeable membrane of the inner tube and dissolve into the liquid carried within. However, because of the semipermeable nature of the inner tube material, the liquid is unable to cross in the opposite direction. The Gas Addition Module is compatible with the Uniqsis FlowSyn and other continuous flow reactors. It can also be used as a reactor in its own right.
More: www.uniqsis.com
Ventana Medical Systems
BenchMark ULTRA with URA
The new BenchMark ULTRA with Ultimate Reagent Access (URA) allows users to process individual immunohistochemistry and in situ hybridization samples as soon as they arrive in the lab. This enhancement allows quick responses to unpredictable changes in workflow, improving test turnaround time and reducing stress associated with laboratory variability. The new software and hardware package enables full workflow control by allowing access to any slide, any reagent, anytime. URA eliminates the need to batch slides, reduces preplanning requirements, and allows late-arriving slides to be run STAT. BenchMark ULTRA with URA also allows users to add and remove slides and reagents at anytime throughout the day.
More: www.benchmarkultra.com
Instrumentation and Hardware
Asynt
3-Way Water Manifold for Asynt DrySyn MULTI
This new DrySyn Water Manifold allows users to connect three water-cooled condensers to a single water supply and DrySyn multiposition heating blocks enable reactions in up to three flasks to be carried out in parallel. Maximizing fume cupboard coolant use, the DrySyn Water Manifold converts a single water tap into three condensing streams, and collects the outlets into one drain tube. With the main body constructed from anodized aluminum, the manifold is designed to be clamped to fume hood scaffolding in either vertical or horizontal alignment. Quick release plastic connectors enable rapid reaction setup and dismantling, with sealing on both the tubing and the manifold side. This means that one condenser can be disconnected while the others remain in use, without leaks. The modular DrySyn MULTI system can convert any standard hotplate stirrer into a reaction block for three flasks of 10–100 mL, or up to 12 vials.
More: www.asynt.com
Beckman Coulter
Allegra X-30 Series Benchtop Centrifuge
The medium capacity Allegra X-30 Series Benchtop Centrifuge from Beckman Coulter is a compact, 18-in.-wide instrument that is available individually or packaged with the appropriate rotors and labware for a range of applications. Two new rotors are available in conjunction with the Allegra X-30 instruments: the SX4400, a 1.6-L swinging-bucket rotor and the S6096 microplate rotor that accommodates 3 × 2 microplates or 2 × 1 deep-well plates. Beckman Coulter offers the new centrifuge with the swinging-bucket rotor and 15- and 50-mL conical tube adapters to create a cell-culture application package. Blood sample processing and microplate application packages also provide everything needed—centrifuge, rotor, and adapters. The fixed-angle rotor achieves 18,000 rpm and 30,000 × g; and the microplate rotor performs at 4700 rpm and 2721 × g. The swinging-bucket rotor's maximum speed is 4700 rpm, with rcf of 4255 × g. In total, the Allegra X-30 accepts 13 different rotors and a range of labware.
More: www.beckmancoulter.com
Biochrom
EZ Read 400 Flexi Reader
New to Biochrom's microplate reader portfolio is the EZ Read 400 Flexi Reader, which is designed to deliver high-quality absorbance measurements and accommodate specialized applications. Customers can select up to four filters of any wavelength from 400 to 750 nm, in addition to four standard ELISA filters of 405, 450, 492, and 620 nm. The EZ Read Flexi Reader is PC controlled using Biochrom's ADAP 2.0 software. ADAP 2.0 Basic software is supplied as standard providing straightforward control for endpoint, kinetic, and multiwavelength measurements, and export into Microsoft Excel. For more sophisticated applications, optional ADAP 2.0 Plus software allows users to design methods for instant data analysis using standard curves or cut offs.
More: www.biochrom.co.uk
Biohit
eLINE 0.1–5-μL Electronic Pipette
According to Biohit, this new pipette is suitable for handling 0.1–5 μL of liquid and is targeted especially for molecular biology laboratories. It dispenses liquid from the tip very rapidly, which ensures that the tip is completely emptied making handling of the liquid contamination free, more precise, and faster than with other pipettes.
More: www.biohit.com
BioTek Instruments
Eon Microplate Spectrophotometer
This new microplate spectrophotomer is monochromator based, and like BioTek's Epoch Microplate Spectrophotometer, Eon's monochromator-based optics allow flexible, filter-free 200–999 nm wavelength selection in 1-nm increments, and spectral scanning for 6- to 384-well microplates. An optional cuvette port expands functionality to cuvette-based assays and also aids in assay miniaturization to microplates or the 2-μL volumes used with BioTek's Take3 and new Take3 Trio Micro-Volume Plates. Other features include 4-Zone temperature control with incubation up to 65 °C, and walk-away automation when paired with BioTek's BioStack Microplate Stacker or third-party robotics. Linear, orbital, and double orbital shake modes are customizable for easy assay optimization in biofuel research, cell proliferation, cytotoxicity studies, and other applications. Eon is powered by the new Gen5 2.0 Data Analysis Software.
More: www.biotek.com
ELx405 Select Deep-Well Washer
This robot compatible, full plate washer for automated aspirate and dispense steps in 96- and 384-well plates up to 50 mm high can also accommodate standard 15-mm microplates. The ELx405 Select Deep-Well Washer also features BioTek's Dual-Action manifold for independent and precise aspirate/dispense control, and variable flow rates and angled dispensing for optimized cell washing. Washing protocols may be created and run through the familiar keypad interface or through BioTek's LHC Software. Optional Ultrasonic Advantage integrated ultrasonic cleaners provide manifold self-maintenance, and optional automatic 1-to-4 wash buffer switching further enhances efficiency.
Typical applications that can now be automated include plasmid DNA purification, serum/plasma sample preparation, hematopoietic progenitor cell enumeration, and cell proliferation assays.
More: www.biotek.com
Delfin Technology
ProfiMessage
ProfiMessage is a new system for data acquisition, monitoring, and automation that is modular and uses master and slave devices and a range of different I/O modules to adapt to tasks. Areas of application include laboratory data acquisition and test stand automation. ProfiMessage devices have universal connectivity and their I/O modules provide a range of process interface options. Profibus-DP, Modbus, or CAN-bus enable connections to PLC control systems for data exchange. Analog and digital sensor signals can record data simultaneously to an independent 16 GB memory that enables subsequent data analysis and evaluation. The data memory can be read out using either USB or Ethernet interfaces. Every input and output is differential, high precision, and galvanically isolated. Depending on the type of I/O module being used, each input can be configured separately to measure mV, mA, RTDs, and thermocouples. ProfiMessage is also equipped with digital inputs, for functioning as status or frequency inputs, and digital/analog outputs. Internal software channels enable functions such as limit value monitoring, integration or online computations, and are easy to configure. These enable the devices to be deployed as monitoring and record-keeping systems.
More: www.delphin.com
Falcon Electric
Advanced SNMP/HTTP Agent
The new Falcon MEGA SNMP/HTTP agent enables remote control, monitoring, and operations system shutdown solutions. The new agent consists of stand alone external or an internal circuit card that fits into the communications option slot of any Falcon SG, SSG, or FN Series rackmount and tower uninterruptible power supply (UPS). The agent allows for the connection to any Ethernet LAN. Two USB ports on the agent support the connection of an optional 802.11G Wi-Fi dongle providing wireless LAN connectivity. As an alternate to 802.11G, an optional GSM/GPRS 1900 cellular modem device is available supporting wireless short message service (SMS) communications with the UPS using supplied SMS server software.
An optional NetFeeler II device supports remote temperature, humidity and water measurement. This module connects through ann RJ45 connector located on the MEGA agent. The module further supports connection of up to two additional sensors, consisting of a magnetic switch to monitor the opening of a door, and a smoke detector.
Operating system shutdown client software is provided with the agent supporting the remote shutdown of virtual servers running VMware. Additional remote in-band shutdown clients are provided for MAC OS X and prior, Windows 7 and prior, Windows Server 2008 R2 and prior, Linux and FreeBSD. Unlike comparative products from competitors, Falcon's client shutdown software may be installed on as many computers as required without any additional licensing costs.
The MEGA agent also supports SNMPv3, HTTPS, RADIUS, SSL, and Open SSL for X Windows, full MD5 password hashing and SHA data encryption modes are supported.
More: www.FalconUPS.com
RheoSense
μVISC (micro VISC) Viscometer
With the new HC chip and VROC technology, the μVISC (micro VISC) viscometer supports testing of higher viscosity ranges up to 20,000 mPas or higher. It is considered easy to use for a range of applications, including testing and evaluating materials such as inks, biologics, chemicals, oils, and other liquids.
More: www.rheosense.com
SP Scientific
Virtis AdVantage Plus Freeze Dryer
The Advantage Plus laboratory benchtop freeze dryer now comes with a restyled exterior, easier shelving adjustment, and is equipped with the Wizard 2.0 controller, the same process control software used on larger VirTis freeze dryers. The controller provides the ability to develop a complete freeze drying recipe, together with the flexibility to program and control individual pressure and temperature settings at each step of the process.
With silicone-flooded shelves, it can maintain a precise level of temperature control previously available only in pilot/production scale freeze dryers. A newly designed condenser coil can hold 6 L before defrosting. At just 26 in. (66 cm) wide, the Advantage Plus can enable product to be safely processed on over 3 ft2 (2766 cm2) of usable shelf area.
The system can freeze dry bulk product or be configured for applications in stoppered vials. Side-mounted manifold valves provide the additional flexibility to freeze dry product in flasks. Three refrigeration choices (−53, −70, or −85 °C) are available for the large 6-L capacity condenser, depending on the type of product and solvents being lyophilized. The AdVantage Plus can be configured with up to three fluid-cooled shelves that maintain precise temperature control.
More: www.spscientific.com
T&D Corporation
Wireless Data Recorder RTR-505TC
These new data recorders are compatible with J, K, S, & T type thermocouples and accept industry standard miniature connectors. They can measure and record temperatures from −199 to +1300 °C, and have 15 recording intervals from 1 s to 60 min and a capacity to store 16,000 readings.
Additional features include a large LCD display, IP64 water resistance, and battery life up to 4 years with “L” version. The units have a communication range up to 500 ft, which is extendable with a repeater. They are compatible with all TandD RTR-500 Series Wireless Data Collectors, which allow for data retrieval to a PC, over a network or the Internet, or through a cellular phone system.
More: www.tandd.com
Thermo Fisher Scientific
CryoStar NX70 Cryostat
The new cryostat accommodates a range of cryotomy applications for both clinical and research use. It combines user comfort with Rapid Response Temperature Control and an innovative knife carrier feed to deliver precise section thickness and consistency. Quick and reproducible instrument setup is simple with the CryoStar NX70's intuitive LCD display and electronic touch screen, together with joystick control. Fine adjustment specimen orientation on all axes enables fast and easy specimen alignment. The Temperature Control system adjusts quickly to changes in settings, enabling rapid cooling, and consistent and precise temperature control. Three user-defined favorites enable frequently used temperature settings to be instantly recalled.
The form-fit design of the CryoStar NX70 ensures user comfort, and integrated, motorized height adjustment allows users to work in either a standing or sitting position. Motorized cutting reduces user fatigue and avoids repetitive injury, while the large open chamber with bright, adjustable LED lighting provides optimal visibility during sectioning.
Optional extras include the Cold-D disinfection system for protection against surface contaminants and Vacutome for wrinkle-free sections and waste removal.
Revco UxF Series ULT Freezers
According to Thermo, its new Revco UxF Series ultra-low temperature freezers maximize sample storage capacity and minimize the freezer's footprint. Equipped with a touch-screen control panel that features an event log, health monitoring system and controlled access options, the Revco UxF Series provides a constant update on the integrity and health of the freezer compartment. Five new capacities range in size from 421 to 949 L (14.9–33.5 ft3) and provide a number of options. The cabinet design and vacuum panel insulation allow storage of up to 70,000 2-mL tubes or 118,300 1-mL Thermo Scientific Cryobank tubes. The freezers offer a choice of high-performance or energy-savings modes.
Torrey Pines Scientific
EchoTherm RIC20 Series Chilling/Heating Dry Baths
These Peltier-driven, compact units offer temperatures settable from −20.0 to 110.0 °C and are designed for remote-controlled applications on robotic and benchtop systems using a PC. Any number of RIC20s can be used via the RS232 I/O port on each unit and PC serial ports. Complex temperature/time profiles created by users can be run by leaving the computer attached to the RIC20. Any number of units can be set to any single temperature via a single computer serial port. The unit(s) will continue to run the temperature instructed until told otherwise. The computer can then be used to instruct the next RIC20.
The units measure 6.5″ (165 mm) long by 4.75″ (120 mm) wide by 3.5″ (89 mm) tall without a sample block. Accessories include a variety of precision-made aluminum sample blocks for standard assay plates, deep-well assay plates, centrifuge tubes of all sizes, vials, and most any size test tube. Custom sample blocks can be made for any sample container by request. The RIC20 Series can be set to 0.1 °C and control temperature to 0.1 °C. Temperature settings are stable to 0.2 °C and accurate to 0.5 °C.
Wheaton
Socorex Acura Manual 826 XS Micropipette
According to Wheaton, this new pipette is shorter in design and features an optimized shape-to-size ratio that facilitates drivability of the pipette and improves the precision of hand movement when pipetting into small vials and microtubes. The tightness lip seal reduces activation forces and provides for ultra-soft pipetting to reduce hand fatigue. Sharp plunger stops result in unsurpassed metrologic performances.
The new Acura manual XS micropipette's Justip height adjustment system permits optimal fitting and ejection of a wide selection of tips, and tip ejection becomes with a low-pressure activation button. Tedious calibration procedures become obsolete with its swift-set user calibration system and integrated key and locking mechanism. It is also autoclavable and fully assembled without recalibration.
More: www.WHEATON.com
Labware and Lab Supplies
Air Science USA
Purair 20 Ductless Fume Hood
This new fume hood has a face velocity at 100 fpm to ensure containment of fumes and an alarm that alerts users when airflow falls to unacceptable levels. All mechanisms in the head section of the Purair 20 are on the clean side of the filter to prevent contamination; and switches and electrical components are totally isolated from the dirty airflow and away from any contamination. The work area has a removable spillage tray that can be easily cleaned, and optional integral lighting is available. The Purair 20 is 49″; wide by 27.5″; deep by 47.5″; high. Other sizes are available up to 96″; wide. The main filter can be chosen from 14 different types of carbon, which include specialty media for vapors of organics, solvents, acids, mercury, and formaldehyde. HEPA filters for particulate filtration are also available. The Purair also can be equipped with a secondary back-up filter.
More: www.airscience.com
Computype
TS968 Histology Cassette Label
This new addition to Computype's suite of high-performance, harsh environment-approved labels is resistant to harsh chemicals and extreme temperatures. It requires no additional label attachment equipment and is considered very easy to use. This permanently adhering label also works with automated pathology systems.
More: www.computype.com
Esco
Frontier Duo Fume Hood
The main enhancement made to the EFD-G2 is a change to the baffle and airfoil design to improve containment. The number of provisions for service fixtures and electrical outlets has been increased therefore increasing the model's flexibility to cater to different customer requirements.
More: www.escoglobal.com
Hamilton Robotics
CO-RE Super Hydrophobic Disposable Tips
For the MICROLAB STAR and NIMBUS lines of automated liquid-handling workstations, these new tips feature a special surface treatment that repels water-based fluids. By virtually eliminating retention of liquid on the inner and outer surfaces, these new tips offer higher pipetting precision and accuracy and lower residual volumes than conventional untreated or low-retention polypropylene tips. They reduce waste of reagents and enzymes and conserve samples.
More: www.hamiltonrobotics.com
CO-RE Piercing Tips
These new disposable tips for Hamilton Robotics's MICROLAB STAR and NIMBUS automated liquid-handling platforms are designed to pierce septa, sealing mats, and foils on tubes and plates. They incorporate carbon fiber reinforcement and vertical-ribbing features to increase rigidity and strength, and a tip bore that prevents blockage and ensures consistent pressure. These new piercing tips are available in black conductive for liquid-level detection and can handle volumes up to 250 μL.
More: www.hamiltonrobotics.com
NuAire
LabGard ES Model NU-629 Animal Handling Class II, Type A2 Biological Safety Cabinet
The LabGard ES NU-629 is designed to provide personnel, product, and environmental protection for sensitive animal handling and cage-changing procedures. Now engineered with an ultra-high efficiency DC style ECM motor, LabGard ES offers an energy savings of more than 50% over conventional motor technology. The ECM motor creates an increase in total filter load capacity of 250% to extend the HEPA filter life expectancy from 7 to 10 years. With the added torque, the NU-629 runs quieter with less vibration. An electronic adjustable work surface height from 23.75″; (603 mm) to 38.75″; (984 mm) or optional 42.75″; (1086 mm) allows the unit to be mobile to fit through standard doors while providing a comfortable work surface height. LabGard NU-629 line now introduces a nominal 5 ft (1.5 m) model to complement the 4 ft (1.2 m) and 6 ft (1.8 m) models providing three choices to accommodate your laboratory.
In addition, a 12″; (305 mm) access opening offers greater visibility and reach within the Class II, Type A2 bio-hazard containment through a 105 FPM air barrier. The unidirectional laminar airflow minimizes air turbulence and cross contamination between animals. All contaminated ducts, plenums, and gaskets are under negative pressure or surrounded by negative pressure to eliminate the possibility of cabinet or gasket leaks and to guard against HEPA failure. The LabGard NU-629 also provides a prefiltration system located in the back of the unit to trap large particle such as animal hair before it enters the plenum, which helps enhance the life of the HEPA filters.
More: www.nuaire.com
Saint-Gobain Performance Plastics
Bio-Simplex Carboys
According to Saint-Gobain, it has redesigned the standard laboratory carboy with updated features that include slender body design that requires less laboratory space (three now can fit in the same space as two yet hold 50% more fluid); minimum dead volume for maximum fluid recovery; large top handles and bottom grip areas for secure handling; wide mouth for easy filling and cleaning; volume and material identification marks; and large, easy-to-read graduation marks with ±5% accuracy.
Manufactured from high-quality polypropylene, the carboys are resistant to chemicals and temperatures up to 275 °F (135 °C) and can be sterilized. They are available in four sizes (5-, 10-, 20-, and 40-L) and can be ordered with or without a spigot. The entire system—carboy, cap, and optional spigot—is autoclavable.
More: www.saint-gobain.com
C-Flex ULTRA Pump Tubing
Saint-Gobain Performance Plastics has expanded its line of thermoplastic elastomer (TPE) tubing with the addition of new C-Flex ULTRA pump tubing, a product designed to perform in demanding biopharmaceutical applications such as pumping, single-use assemblies, process filling, and sampling.
Produced from validated C-Flex resin, C-Flex ULTRA pump tubing exceeds requirements for a flexible, clear, weldable, and sealable pump tubing for fluid-processing applications.
According to Saint-Gobain, the C-Flex ULTRA can still pump after 1400 hof continuous operation (at 600 rpms with 15 lb of back pressure). It can deliver lower spallation during the pumping process than any other TPE or silicone formulation; and its smooth outer surface does not gum up pump heads.
TandD Corporation
Log-EZ Wireless Data Collector
Temperature and humidity data recorded by multiple log-EZ units in different rooms can be managed via one computer. The range of each log-EZ is up to 150 ft and up to 16 loggers can be connected to a single system. By daisy-chaining individual loggers, communication over a considerable distance can be achieved. These units have a temperature range of 0–50 °C (±1 °C) and a humidity range of 15–90% RH (±6%RH), which are can be viewed on a large LCD display. The log-EZ records data and can store 1440 readings. Current readings can be viewed in real time on a PC, and recorded data can be automatically downloaded and archived for future reference. The kit includes the RTR-322 Recorder, the RTR-320 USB Transceiver, software, and mounting bracket.
More: www.tandd.com
Microplates
Biotek Instruments
Take3 Trio Micro-Volume High-Density Plate
Take3 Trio is designed for nucleic acid and protein quantification without sample dilution and is capable of measuring up to 48 2-μL samples. Microliter samples are pipetted onto specially coated slides that are simply wiped clean after the samples are read for simple maintenance. Take3 Trio is compatible with most of BioTek's microplate readers, including the new Eon Microplate Spectrophotometer. Gen5 Data Analysis Software contains preprogrammed Take3 Trio protocols, and allows spectral scanning, sample naming, and parallel reading of samples and blanks.
More: www.biotek.com
Informatics and Computing
BioTek Instruments
Gen5 Version 2.0 Data Analysis Software
According to Biotek, Gen 5 Version 2 offers a new interface and new control features that include run time well selection for variable microplate area analysis to streamline the day-to-day use of the software, and enhanced high-definition area scans up to 9800 data points per well to better support cell-based assays. New data analysis functions simplify routine data analysis, and a new formula editor facilitates the Gen5 Version 2.0 data analysis syntax. All reporting and exporting tools are grouped in one logical area, while new automatic content allows simple reporting and exporting. In addition to controlling BioTek's existing microplate readers, Gen5 Version 2.0 supports the new Eon Microplate Spectrophotometer and Take3 Trio Micro-Volume Plate.
More: www.biotek.com
GE Healthcare
IN Cell Analyzer Compliance Manager
IN Cell Analyzer Compliance Manager is a software module for the IN Cell Analyzer 2000 that brings both operating and analysis software to a 21 CFR Part 11 compliant standard. Developed to facilitate compliance with regulatory expectations worldwide, IN Cell Analyzer Compliance Manager is the first 21 CFR Part 11 compliant software from GE Healthcare for high-content analysis, and delivers required functionality such as role-based user authorization levels to set access rights; published procedures for operational control that lock run and evaluation settings together; audit trails to record modifications and maintain histories on procedures; and data integrity and Certificate of Audit. GxP instrument validation services are also available using a risk-based validation approach.
Malvern Instruments
Morphologi G3 Particle Characterization System Software
This fully automated image analysis-based system delivers particle size and shape information for wet and dry samples, and for the analysis of foreign particulate matter collected on filters. Among the new applications in the version 7.40 software are facilities for user-defined light settings and automated dark-field measurements, further extending the range of applications for which the Morphologi G3 can be used. A new reanalyze function enables the application of filters, classes, and the rebinning of data to multiple records for improved productivity and workflow. With the new software, Morphologi G3 users can more closely define their own light settings in the standard operating procedures, allowing for dark-field and polarizer measurements. The Version 7.40 software also introduces a polygonal selection tool for tighter definition of size and shape classes, and a circular area selection tool for more accurate analysis of particulates on filters.
Two-Fold Software
Qualoupe LIMS
A new secure web reporting capability generates on-demand reports from lab data. Internal reports can be run from within each Qualoupe application. For example, within the Sample Manager module the reports would all be predominately sample centric. When running the reports, users can select the current record set, or single record, or run a new query. A secure access interface enables secure downloading by authorized users.
More: www.twofold-software.com
Services
Advion BioServices
SISCAPA LC/MS Biomarker Assay Services
This new service is made possible by a license and collaboration agreement between Advion BioServices and SISCAPA Assay Technologies. The SISCAPA assays are mass spectrometry based, and are conducted in Advion's LC/MS laboratory in Ithaca, NY.
More: www.advion.com
American Type Culture Collection
STR Profiling Service for Human Cell Line Authentication
To verify the identity of human cell lines via short tandem repeat profiling (STR profiling) cells are applied to an FTA card provided with an FTA Sample Collection Kit from American Type Culture Collection (ATCC), which eliminates the need to ship samples on dry ice. ATCC will amplify the DNA using the Promega PowerPlex 16 HS System and analyze the results, which deliver via e-mail within 5 days from receipt of samples.
ATCC offers two levels of service: Basic and Verified. The Basic STR Profiling Service (135-X) includes a signed Cell Line Authentication Report containing an STR allele table and electropherograms that support the allele calls at each locus. The Verified STR Profiling Service (135-XV) includes everything in the Basic service, plus known reference profiling against the ATCC STR Profile database, and a comprehensive interpretation of the results.
ATCC generates the STR profile by simultaneously amplifying 15 STR loci, plus the amelogenin gene for gender determination, in a multiplex PCR reaction enabling a 1 in >1017 matching rate for unrelated individuals.
Beckman Coulter Genomics
Automated, High-Capacity, Target Capture Sequencing
Beckman Coulter Genomics now offers a completely automated, high-capacity, target capture sequencing service that delivers quality sequencing data and streamlines sample preparation for targeted next-generation sequencing. Using Agilent SureSelect Target Enrichment technology with the Beckman Coulter SPRIworks Fragment Library System for automated library construction and the Biomek Laboratory Automation Workstation for liquid handling provides high-quality, reproducible samples ready for sequencing. Sequencing is performed on the Illumina GAII and HiSeq 2000 platforms. LIMS sample tracking also enables large numbers of samples to be processed at the same time.
More: www.beckmangenomics.com/genomic_services/dna_sequencing/automated_target_capture.html
Warwick Analytical Service
Micro Determination of Fluorine
Operating in an ISO 17025 and GMP accredited environment, Warwick Analytical Service (WAS) now offers proprietary analytical procedures to enable accurate and precise data to be produced for all types of samples including organic samples with trace levels of Fluorine. From extensive method validation, typical results are within the ±0.3% absolute limits considered acceptable for micro chemical analysis. The WAS method can detect 0.01 ppm fluoride, which is equivalent to 0.02% in a 10-mg sample. WAS has developed special procedures to remove interferences from samples containing boron, calcium, or sulfur.
Analysis of Fluorine is carried out by combustion of an accurately weighed sample in a silica oxygen flask over an accurately measured volume of water and TISAB buffer solution. A fluoride ion selective electrode is used to directly measure the potential of a range of fluoride solutions. The potential of the combusted samples are measured under the same conditions. The concentrations of the sample solutions are calculated from the derived calibration curve. Sets of work typically include certified reference materials or organic analytical standards. Results from these standards and the electrode response are monitored to detect trends in the method.