Correction: Cellular Reprogramming: 2012;14/6:485–496

In the article “Induced Pluripotent Stem Cells Generated from Adult Bone Marrow-Derived Cells of the Nonhuman Primate (Callithrix jacchus) Using a Novel Quad-Cistronic and Excisable Lentiviral Vector” (published in Cellular Reprogramming: 2012; 14/6:485–496 by A. Wiedemann, K. Hemmer, I. Bernemann, G. Gohring, O. Pogozhykh, C. Figueiredo, S. Glage, A. Schambach, J.C. Schwamborn, R. Blasczyk, and T. Muller, the authors discovered a mistake concerning a primer pair and the corresponding PCR picture. The text and correct figure are as follows.

The oligonucleotide sequences, picture, and PCR conditions for the excision verification are incorrect. The correct oligonucleotide sequences are identical as in Voelkel et al., 2010 (5′-CGA GTC GGA TCT CCC TTT GGG C - 3′; 5′-GGT TCC CTA GTT AGC CAG AGA GC-3′ and 5′-TGG AAG GGC TAC GTA GCT AGC-3′). Polymerase chain reaction was performed as follows: 1 cycle at 94°C/180 sec, followed by 34 cycles at 94°C/60 sec, annealing at 60°C/45 sec, elongation at 71°C/60 sec, and the final step at 72°C/10 min.

The authors regret this error.

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Primers 1 and 3 produce a 290-bp amplicon in case of remaining OCT3/4, KLF4, SOX2, and C-MYC (OKSM) cassette; primers 2 and 3 produce a 170-bp band regardless of removal of the cassette. Marmoset embryonic stem cells (ESCs) and H₂O served as controls. OKSM, lentiviral vector used for reprogramming with FRT-sites in the δU3 region of the long terminal repeat (LTR); cjiPS, Callithrix jacchus induced pluripotential stem cells (iPSCs); cjiPSEX, Callithrix jacchus iPSCs after extraction of the OKSM cassette; hsiPS, Homo sapiens iPSCs after extraction (control from Voelkel et al., 2010); ESC, Callithrix jacchus embryonic stem cell line cj001; H₂O, water.