Abstract
A human genomic DNA fragment in phage λ containing FSHB, the gene for the β-subunit of human follicle-stimulating hormone (FSH-β), was analyzed and the nucleotide sequence of the region of the clone encoding FSH-β was determined. A subclone of the λ phage containing 67% of FSH-β coding sequence was used as hybridization probe to determine the human chromosomal location of FSHB. A panel of mouse-human somatic cell hybrids containing reduced numbers of human chromosomes was screened with the FSHB probe; complete cosegregation of FSHB with human chromosome 11 was observed in all 26 cell hybrids tested. Analysis of a set of cell hybrids containing translocated derivatives of chromosome 11 further localized FSHB to the human chromosome region llpll.2→11pter. A Hind III restriction fragment length polymorphism (RFLP) detected by another subclone of the λ phage containing FSHB now provides a genetic marker for this region of the human genome.
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