Transcriptional Activation of the Alpha-1 Acid Glycoprotein Gene by YY1 Is Mediated by Its Functional Interaction with a Negative Transcription Factor

Regulation of alpha-1 acid glycoprotein (AGP) gene expression involves both positive and negative transcription factors. We have previously identified two dominant factors: positive and negative transcription factors, AGP/EBP and factor B, respectively, involved in the transcription of AGP and other acute-phase response genes. In this report, we present evidence showing that the transcription of the AGP gene is positively regulated by a transcription factor, YY1. The activation of AGP gene by YY1 is mediated by a negative element B in the AGP promoter region. YY1 can also activate the B motif linked to a heterologous promoter. However, YY1 does not bind directly to the B motif per se. Rather, our data suggest that the activation of AGP gene by YY1 may be mediated by its functional interaction with factor B, which recognizes the B motif.