Anti-Peptide Monoclonal Antibodies to the β-Adrenergic Receptor: Use in Purification of β Receptor 1,2

This article describes a new immunopurification procedure based on monoclonal antibodies raised against peptides of the carboxy-terminal region of the turkey β-adrenergic receptor. This procedure constitutes a significant purification step of recombinant β-adrenergic receptors expressed in baculovirus-infected Sf9 cells, and allows the recovery of receptors able to activate G_s in phospholipid vesicles. Additionally, this procedure can be combined with affinity chromatography to yield nearly homogeneous receptor.