Abstract
Antigen Used for Immunization
Antigen used for immunization is prokaryotic expressed GST-ppGalNAcT2 protein, which was cloned from ppGalNAc-T2-PGEX-5x-3.
Method of Immunization
Rabbits were immunized three times by GST-ppGalNAcT2 fusion protein together with complete Freund's adjuvant every 2 to 3 weeks.
Parental Cell Line Used for Fusion
Not available.
Selection and Cloning Procedure
Not available.
Heavy and Light Chains of Immunoglobulin
Not available.
Specificity
This antibody could be used to identify human polypeptide:N-acetylgalactosaminyltransferase-2 ppGalNAcT2 of cancer cells by Western blot analysis and immunochemistry.
Specific Antigen Identified
Human polypeptide:N-acetylgalactosaminyltransferase-2 ppGalNAcT2.