Recombinant mouse Nup96 protein fragment produced in Escherichia coli.
Method of Immunization
WKY/IZM rat was injected via the hind footpads with 200 μL of an emulsion containing 400 μg of recombinant mouse Nup96 protein fragment and Freund's complete adjuvant.
Parental Cell Line Used for Fusion
SP2/0.
Selection and Cloning Procedure
Monoclonal antibody was selected by HAT media, and positive clone identification was done by ELISA.
Heavy and Light Chains of Immunoglobulin
Unknown.
Specificity
The specificity was determined by Western blot analysis. This MAb exclusively recognizes Nup96 among nucleoporins.
Specific Antigen Identified
Human, mouse Nup96.
Availability
Tissue culture supernatant
Yes ✓
No
Ascitic fluid
Yes
No ✓
Hybridoma cells
Yes
No ✓
References
1.
MizuguchiC, OkaM, MoriyamaT, TachibanaT, YonedaY. Specific monoclonal antibody against the nuclear pore complex protein, Nup96. Hybridoma, 2010; 29,6.
