Female Balb/c mice were injected intraperitoneally with 400 μL of a 1:1 emulsion of procalcitonin fusion protein (30 μg per mouse) and complete Freund's adjuvant and then boosted with the antigen in incomplete Freund's adjuvant at 2-week intervals. After the mice received four booster shots, the immunological response was assessed by ELISA based on the titer. Prior to fusion, the mice were again boosted with 30 μg of the antigen in phosphate-buffered saline (PBS, pH 7.2).(1)
Parental Cell Line Used for Fusion
SP2/0.
Selection and Cloning Procedure
ELISA/limiting dilution.
Heavy and Light Chains of Immunoglobulin
IgG1, kappa.
Specificity
Enzyme-linked immunosorbent assay (ELISA) was carried out to analyze the specificity of hybridoma cell lines.
Specific Antigen Identified
Unknown.
Availability
Tissue culture supernatant
Yes
No ✓
Ascitic fluid
Yes ✓
No
Hybridoma cells
Yes ✓
No
References
1.
MaQ, GengY, XuW, WuY, HeF, ShuW, HuangM, DuH, LiM. The role of translationally controlled tumor protein in tumor growth and metastasis of colon adenocarcinoma cells. J Proteome Res, 2010; 9,1:40–49.
