Recombinant human HB-EGF protein expressed in HEK293T cells.
Method of Immunization
BALB/c mice were immunized with purified recombinant HB-EGF extracellular domain.
Parental Cell Line Used for Fusion
P3U1.
Selection and Cloning Procedure
The clones were selected using antigen ELISA and then flow cytometry by using 293T cells expressing human HB-EGF.
Heavy and Light Chains of Immunoglobulin
IgG1.
Specificity
Specificity was checked by cell-based binding assay and Western blotting. This antibody reacts with human HB-EGF but not mouse HB-EGF. No cross-reactivity to other EGFR ligands was observed by antigen ELISA.
Specific Antigen Identified
Human HB-EGF protein.
Availability
Commercially available from MBL Co., LTD.
Yes
No
Tissue culture supernatant
[ ]
[✓]
Ascitic fluid
[ ]
[✓]
Hybridoma cells
[ ]
[✓]
References
1.
Iwamotoet al.Characterization of a novel anti-human HB-EGF monoclonal antibody applicable for paraffin-embedded tissues and diagnosis of HB-EGF-related cancers. Monoclon Antib Immunodiagn Immunother, 2016; 35(2):73–82.
