Correction to: Impact of Mothers' Age on Telomere Length and Human Telomerase Reverse Transcriptase Expression in Human Fetal Membrane-Derived Mesenchymal Stem Cells by Alrefaei GI,Alkarim SA,and Abduljabbar HS. Stem Cells Dev 2019;28;24;1632

Abstract

The authors of the article entitled, Impact of Mothers' Age on Telomere Length and Human Telomerase Reverse Transcriptase Expression in Human Fetal Membrane-Derived Mesenchymal Stem Cells, published in Stem Cells Dev 2019;28;24:1632–1645 wish to correct errors that appeared in Figure 1 and Table 3.

FIG. 1.

Cellular morphology of hFM-MSCs. Representative images of hFM-MSCs [decidua basalis (hDB), chorionic plate (hCP), amniotic membrane (hAM), and umbilical cord Wharton's jelly (hWJSCs)] from the three different age groups during cell culture at the initial passage (P0) and late passage (P6). At day 3 and 6, hFM-MSCs appear short, then from day 9 until 6th passages the cells appear fibroblast-like cells. They were longer, flattened, and have an expanded cytoplasm. (magnification × 10). hFM-MSCs, human fetal membranes–mesenchymal stem cells. Color images are available online.

FIG. 1.

Table 3.

Comparison of hFM-MSCs Proliferation Rates in Different Studied Groups by Doubling Time

Groups Parameters	Control Samples			hMSCs 30–39 years (n = 16)	hMSCs ≥40 years (n = 16)	Mean square	F-Factor	Significance
Groups Parameters	hMSCs 20–29 years (n = 16)	K562 (n = 16)	hBM-MSCs (n = 16)	hMSCs 30–39 years (n = 16)	hMSCs ≥40 years (n = 16)	Mean square	F-Factor	Significance
Decidua basalis (hDB)	45.90 ± 0.01	19.39 ± 0.31	75.42 ± 0.04	44.27 ± 0.02	43.98 ± 0.01	1580.517	78295.110	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
			2P < 0.05	3P < 0.05	3P < 0.05
Chorionic plate (hCP)	46.59 ± 0.02	19.39 ± 0.31	75.42 ± 0.04	45.15 ± 0.04	44.90 ± 0.01	1575.452	76751.501	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
			2P < 0.05	3P < 0.05	3P < 0.05
Amniotic membrane (hAM)	45.50 ± 0.01	19.39 ± 0.31	75.42 ± 0.04	44.18 ± 0.01	43.93 ± 0.02	1580.997	75142.437	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
			2P < 0.05	3P < 0.05	3P < 0.05
Umbilical cord Wharton's jelly (hUCWJ)	46.59 ± 0.07	19.39 ± 0.31	75.42 ± 0.04	45.22 ± 0.03	44.97 ± 0.02	1575.158	74510.786	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
			2P < 0.05	3P < 0.05	3P < 0.05

Data are given as mean ± standard deviation to compare hMSCs growth rates of hFM-MSCs (D) with different studied groups by doubling time (DT). Significance was determined using multivariate analysis and then significant data were analyzed by one-way analysis of variance (LSD) test. P < 0.05 was considered significant. IP, significance versus hMSC from GI of the same region; 2P, significance versus K562; 3P, significance versus hBM-MSC, 4P, significance versus hMSC from GII of the same region.

Table 3.

Comparison of hFM-MSCs Proliferation Rates in Different Studied Groups by Doubling Time

Groups Parameters	Control Samples			hMSCs 30–39 years (n = 16)	hMSCs ≥40 years (n = 16)	Mean square	F-Factor	Significance
Groups Parameters	hMSCs 20–29 years (n = 16)	K562 (n = 16)	hBM-MSCs (n = 16)	hMSCs 30–39 years (n = 16)	hMSCs ≥40 years (n = 16)	Mean square	F-Factor	Significance
Decidua basalis (hDB)	45.90 ± 0.01	19.39 ± 0.31	75.42 ± 0.04	44.27 ± 0.02	43.98 ± 0.01	1580.517	78295.110	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
				3P < 0.05	3P < 0.05
					4P < 0.05
Chorionic plate (hCP)	46.59 ± 0.02	19.39 ± 0.31	75.42 ± 0.04	45.15 ± 0.04	44.90 ± 0.01	1575.452	76751.501	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
				3P < 0.05	3P < 0.05
					4P < 0.05
Amniotic membrane (hAM)	45.50 ± 0.01	19.39 ± 0.31	75.42 ± 0.04	44.18 ± 0.01	43.93 ± 0.02	1580.997	75142.437	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
				3P < 0.05	3P < 0.05
					4P < 0.05
Umbilical cord Wharton's jelly (hUCWJ)	46.59 ± 0.07	19.39 ± 0.31	75.42 ± 0.04	45.22 ± 0.03	44.97 ± 0.02	1575.158	74510.786	0.001
Significance		1P < 0.05	1P < 0.05	1P < 0.05	1P < 0.05
			2P < 0.05	2P < 0.05	2P < 0.05
				3P < 0.05	3P < 0.05
					4P < 0.05

In Figure 1, repetition occurred in day 9, where the hDB-MSCs - GiII (≥40 years) was the same as hAM-MSCs - GII (30–39 years) and hCP-MSCs - GI (20–29 years) was the same as hUCWj - MSCs - GI (20–29 years).

The original figure is on the left. The authors have revised Figure 1 as shown on the right.

In Table 3, the column hMSCs ≥40 (n = 16), “4P” has been added to all groups.

The original Table 3 is on top and the revised Table 3 is shown on the bottom.

The online version of this article has been revised to reflect these corrections.

The authors sincerely regret these errors. For additional information, please contact: Dr. Ghadeer I. Alrefaei, Biology Department, Faculty of Sciences, University of Jeddah, Jeddah 22264, Saudi Arabia. E-mail: galrefaei@uj.edu.sa

Correction to: Impact of Mothers' Age on Telomere Length and Human Telomerase Reverse Transcriptase Expression in Human Fetal Membrane-Derived Mesenchymal Stem Cells by Alrefaei GI,Alkarim SA,and Abduljabbar HS. Stem Cells Dev 2019;28;24;1632–1645 DOI:10.1089/scd.2019-0144

Abstract