Abstract


Analysis of flow cytometry experiments examining the effect of TSHR N-terminal loop 1 deletion on M22 and control antibody recognition. Although M22 and control antibodies were not always tested together (as in Figs. 2 and 3), in all experiments each antibody was tested in parallel with stably expressed wild-type TSHR-ECD-GPI and Δ22–30-TSHR-ECD-GPI cell lines. For inter-experiment comparison, the ratio of maximum fluorescence (mean ± SE) with each cell line is shown for control antibody 2C11 and for M22 (mean ± SE for 5 experiments and 10 experiments, respectively; p = 0.02, Student's t-test).
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