Abstract
US 2011/0129898 A1 Patent application Published 2 June 2011
Methods and Compositions for Increasing Nuclease Activity
Applicants/Inventors
Disclosed herein are methods and compositions for increasing nuclease activity to increase nuclease-mediated genomic modifications.
US 2011/0129472 A1 Patent application Published 2 June 2011
Methods for Controlling Vasculogenesis
Applicants/Inventors
The present invention relates to a method for detecting the presence and/or progress of vasculogenesis in a subject, said method comprising the steps of detecting the presence of activated endothelial progenitor cells in a sample of a circulation fluid of said subject.
US 2011/0123534 A1 Patent application Published 26 May 2011
Novel Compounds for Modulating Neovascularisation and Methods of Treatment Using These Compounds
Applicants/Inventors
The invention relates to a method for modulating neovascularisation comprising administering to a subject one or more compound selected from an isolated nucleic acid molecule comprising a gene selected from the group consisting of RIKEN cDNA 9430020K01, Agtrl1, Apelin, Stabilin 1, Stabilin 2, TNFaip8l1, TNFaip8, and FGD5, and homologs thereof; a gene product encoded by said genes or their homologs genes, and functional fragments thereof; an antibody or derivative thereof directed against a gene product of said genes or their homologs genes, and functional fragments thereof; an antisense molecule capable of binding to a gene or an mRNA gene product of said genes and homologs thereof; a small molecule interfering with a biological activity of a gene product of said genes and homologs thereof, and a (glyco)protein, a hormone and other biologically active compounds capable of interacting with said genes and homologs thereof or with a gene product thereof.
US 2011/0126300 A1 Patent application Published 26 May 2011
High-Throughput Method of Screening Compounds for Pharmacological Activity
Applicants/Inventors
Provided is a method for high-throughput screening for physiologic alterations in an altered teleost displaying a phenotype that is characteristic of the alteration and different from a wild-type, unaltered, matched teleost, comprising the steps of contacting the teleost displaying a genetically-inherited or chemically induced phenotype with at least one test compound for a sufficient time and under suitable conditions to induce a response in the teleost indicative of pharmacological activity of the compound, and detecting and comparing the response with that of a matched, untreated control, wherein a change in the teleost signal that is different from that of the control indicates an altered phenotype and pharmacological activity of the at least one test compound. Further provided are the compounds identified by this method, the zebrafish having an altered phenotype resulting from treatment in accordance with these methods, and kits for facilitating the high-throughput screening methods.
WO 2011/059826 A2 Patent application Published 19 May 2011
Multiple-Photon Excitation Light Sheet Illumination Microscope
Applicants/Inventors
An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in four-dimensional imaging of live biological systems.
www.patentlens.net/patentlens/patent/WO_2011_059826_A2/en/
Also see United States patent application US 2011/0122488 A1 published 26 May 2011
WO 2011/059833 A2 Patent application Published 19 May 2011
Dual-Mode Raster Point Scanning/Light Sheet Illumination Microscope
Applicants/Inventors
An apparatus for and method of performing light sheet microscopy (LISH) and light scanning microscopy (Raster Point Scanning [RAPS]) in a single device are provided. The dual-mode imaging microscope allows for the use of both LISH and RAPS in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples. In addition, the device will reduce the high costs and space requirements associated with owning two different microscopes (LISH and RAPS).
US 2011/0099648 A1 Patent application Published 28 April 2011
Recombinant Gene That Enhances the Ability of Fish to Tolerate Low Dissolved Oxygen Stress and the Use Thereof
Applicants/Inventors
The invention discloses a recombinant gene that enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, that is, blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.
US 7919583 Granted patent Published 5 April 2011
Integration-Site-Directed Vector Systems
Applicants/Inventors
Some aspects of the application describe materials and methods for making a molecular tether. A molecular tether, in certain embodiments, includes a target-DNA-binding domain having a specific binding affinity for a target-DNA segment in a host chromosome, a carrier-binding domain that specifically binds to a DNA segment on a carrier, and a spacer covalently bonded to the target DNA-binding domain and the carrier-binding domain.
Footnotes
Ms. Westcot is a J.D./Ph.D. candidate in the Joint Degree Program in Law, Health, and Life Sciences at the University of Minnesota–Twin Cities, Minneapolis, Minnesota. The contents of this column do not constitute legal advice.
All patent information is reproduced with permission from CAMBIA Patent Lens—a free full-text patent search database.