Antibacterial activity of PA6/ZnO nanocomposite fibers

Abstract

In this study, ZnO-loaded PA6 nanocomposite preparation and its antibacterial activity are investigated. This work aims to study the effect of the sizes and amount of the ZnO nanofiller on the antibacterial, mechanical, and thermal properties of the PA6/ZnO nanocomposites. The melt intercalation method is applied to prepare polyamide 6 (PA6) nanocomposite fibers, including 0, 0.5, 1, 3, 5 wt % zinc oxide (ZnO), using a laboratory-scale compounder. The antibacterial activity of the fibers against Staphylococcus aureus (ATCC 6538) as a gram positive bacterium and Klebsiella pneumoniae (ATCC 4352) as a gram negative bacterium is determined according to ASTM E 2149-0. Mechanical and thermal characterization tests are performed according to relevant standards (ASTM D7426-08, ASTM E1131-08, ASTM D3822-07; DSC, TGA, tensile tests). It is found that the dispersion of the ZnO particles within the PA6 matrix is homogenous according to scanning electron microscopy results. Antibacterial activity tests show that PA6/ZnO nanocomposite fibers exhibit antibacterial efficiency related to their nanoparticle contents. An increase in the amount of nanoparticles causes an increase of the antibacterial activity of the fibers. On the other hand, mechanical and thermal characterization tests show that the addition of ZnO nanoparticles does not affect the strength and thermal properties of the nanocomposites for these loadings.

Keywords

antibacterial nanocomposite polyamide 6 zinc oxide

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References

Paul DR and Robeson LM Polymer nanotechnology: nanocomposites. Polymer 2008; 49: 3187-3204.

Sawhney APS , Condon B. , Singh KV , Pang SS , Li G. and Hui D. Modern application of nanotechnology in textiles. Textile Research Journal 2008; 78: 731-739.

Gowri S. , Almeida L. , Amorim T. , Carneiro N. , Souto AP and Esteves MF Polymer Nanocomposites for multifunctional finishing of textiles - a review . Textile Research Journal 2010; 80: 1290-1306.

Dastjerdi R. , Mojtahedi MRM , Shoshtari AM , Khosroshahi A. and Moayed AJ Fiber to fabric processability of silver/zinc-loaded nanocomposite yarns. Textile Research Journal 2009; 79: 1099-1107.

Gao Y. and Cranston R. Recent advances in antimicrobial treatments of textiles. Textile Research Journal 2008; 78(1): 60-72.

Ramachandran T. , Rajendrakumar K. and Rajendran R. Antimicrobial textiles - an overview. IE(I)-TX 2004 ; 84: 42-47.

Deopura BL , Alagirusamy R , Joshi M and Gupta B (eds) Polyester and polyamides . Cambridge: Woodhead Publishing in Textile, 2008.

McIntyre JE (ed.) Synthetic fibres: Nylon, Polyester, Acrylic, Polyolefin. Cambridge: Woodhead Publishing in Textile, 2005.

Damm C. , Munstedt H. and Rosch A. The antimicrobial efficacy of polyamide 6/silver-nano-and microcomposites . Mater Chem Phys 2008; 108: 61-66.

10.

Kathirvelu S. , D’souza L. and Dhurai B. A study on functional finishing of cotton fabrics using nano-particles of zinc oxide. Mater Sci 2009; 15: 75-79.

11.

Turkoglu M. and Yener S. Design and in vivo evaluation of ultrafine inorganic-oxide-containing-sunscreen formulations. Int J Cosm Sci 1997; 19: 193-201.

12.

Pan ZW , Dai ZR and Wang ZL Nanobelts of semiconducting oxides. Science 2001; 291: 1947-1949.

13.

Arnold MS , Avouris P. , Pan ZW and Wang ZL Field-effect transistors based on single semiconducting oxide nanobelts . J Phys Chem B 2003; 107: 659-663.

14.

Sawai J. Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. J Microbiol Meth 2003; 54: 177-182.

15.

Xiong M. , Gu G. , You B. and Wu L. Preparation and characterization of poly(styrene butylacrylate) latex/ nano-ZnO nanocomposites. J Appl Polym Sci 2003; 90: 1923-1931.

16.

Li HJ , Hong RY , Li MY , Li HZ , Zheng Y. and Ding J. Effects of ZnO nanoparticles on the mechanical and antibacterial properties of polyurethane coatings. Prog Org Coat 2009 ; 64: 504-509.

17.

Ghule K. , Ghule AV , Chen BJ and Ling YC Preparation and characterization of ZnO nanoparticles coated paper and its antibacterial activity study. Green Chem 2006 ; 8: 1034-1041.

18.

Sawai J. , Igarashi H. , Hashimoto A. , Kokugan T. and Shimizu M. Evaluation of growth inhibitory effect of ceramics powder slurry on bacteria by conductance method. J Chem Eng Jpn 1995 ; 28: 288-293.

19.

Sawai J. , Saito I. , Kanou F. , Igarashi H. , Hashimoto A. , Kokugan T. , et al. Mutagenicity test of ceramic powder which have growth inhibitory effect on bacteria. J Chem Eng Jpn 1995; 28: 352-354.

20.

Jones N. , Ray B. , Ranjit KT and Manna AC Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganism. FEMS Microbiol Lett 2008 ; 279: 71-76.

21.

Li X. , Xing Y. , Jiang Y. , Ding Y. and Li W. Antimicrobial activities of ZnO powder coated PVC film to inactivate food pathogens. Int J Food Sci Technol 2009 ; 44: 2161-2168.

22.

Yeo YS , Lee HJ and Jeong SH Preparation of nanocomposite fibers for permanent antibacterial effect . J Mater Sci 2003; 38: 2143-2147.

23.

Guo L. , Yang S. , Yang C. , Yu P. , Wang J. , Ge W. , et al. Synthesis and characterization of poly(vinylpyrrolidone)-modified zinc oxide nanoparticles. Chem Mater 2000; 12: 2268-2274.

24.

Kwon YJ , Kim KH , Lim CS and Shim KB Characterization of ZnO nanopowders synthesized by the polymerized complex method via an organochemical route. J Ceram Process Res 2002; 3: 146-149.

25.

Wang Y. , Ma C. , Sun X. and Li H. Preparation of nanocrystalline metal oxide powders with the surfactant mediated method. Inorg Chem Comm 2002; 5: 751-755.

26.

Liufu S. , Xiao H. and Li Y. Investigation of PEG adsorption on the surface of zinc oxide nanoparticles . Powder Technol 2004; 145: 20-24.

27.

Zhang LL , Jiang YH , Ding YL , Povey M. and York D. Investigation into the antibacterial behavior of suspensions of ZnO nanoparticles (ZnO nanofluids). J Nanopart Res 2007; 93: 479-489.

28.

Sawai J. , Igarashi H. , Hashimoto A. , Kokugan T. and Shimizu M. Effect of particle size and heating temperature of ceramic powders on antibacterial activity of their slurries. J Chem Eng Jpn 1996; 29: 251-256.

29.

Makhluf S. , Dror R. , Nitzan Y. , Abramovich Y. , Jelinek R. and Gedanken A. Microwave-assisted synthesis of nanocrystalline MgO and its use as bacteriocide . Adv Funct Mater 2005; 15: 1708-1715.

30.

Yamamoto O. , Hotta M. , Sawai J. , Sasamoto T. and Kojima H. Influence of powder characteristic of ZnO on antibacterial activity - effect of specific surface area. J Ceram Soc Jpn 1998; 106: 1007-1011.

31.

Sawai J. , Kawada E. , Kanou F. , Igarashi H. , Hashimoto A. , Kokugan T. , et al. Detection of active oxygen generated from ceramic powders having antibacterial activity. J Chem Eng Jpn 1996 ; 29: 627-633.

32.

Sawai J. , Shoji S. , Igarashi H. , Hashimoto A. , Kokugan T. , Shimizu M. , et al. Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry. J Ferment Bioeng 1998; 86: 521-522.

33.

Anpo M. , Shima T. , Kodama S. and Kubokawa Y. Photocatalytic hydrogenation of propyne with water on small-particle titania: size quantization effects and reaction intermediates. J Phys Chem 1987; 91: 4305-4310.

34.

Reddy KM , Feris K. , Bell J. , Wingett DG , Hanley C. and Punnoose A. Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Appl Phys Lett 2007; 90(213902): 213902-1-213902-3.

35.

Heinlaan M. , Ivask A. , Blinova I. , Dubourguier HC and Kahru A. Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus. Chemosphere 2008; 71: 1308-1316.