The effects of nitric oxide (NO) on chilling tolerance (contents of hydrogen peroxide (H2O2) and superoxide anion (O2–) and lipid peroxidation level (malondialdehyde, MDA)) and the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT)) were investigated in the leaves of wheat (Triticum aestivum L.) exposed to chilling. NO treatment was carried out through spraying of sodium nitroprusside (SNP), which is a donor of NO. To do this, SNP concentrations of 0.1 and 1 mM were applied on the leaves of 11-day plants and the plants were then exposed to chilling conditions (5/2°C) for 3 days. The chilling stress treatment increased both the activities of antioxidant enzymes and the levels of MDA, H2O2 and O2–. Similarly, NO treatment enhanced SOD, POX and CAT activities under chilling stress, whereas it decreased H2O2 and O2.– contents as well as MDA level. The most effective concentration was determined as 0.1 mM SNP. Exogenous SNP application as a donor of NO was found to have an important ameliorative effect on cold tolerance of seedling exposed to chilling stress by stimulating antioxidant enzyme activity.
AticiONalbantogluB (2003) Antifreeze proteins in higher plants. Phytochemistry6: 1187–1196.
2.
BeligniMVLamattinaL (1999) Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues. Planta208: 337–344.
3.
CuiXMZhangYKWuXBLiuCS (2010) The investigation of the alleviated effect of copper toxicity by exogenous nitric oxide in tomato plants. Plant Soil Environment56: 274–281.
4.
DelledonneMXiaYJDixonRALambC (1998) Nitric oxide functions as a signal in plant disease resistance. Nature394: 585–588.
5.
DhindsaRAPlumb-DhindsaTA (1981) Thorpe, leaf senescence correlated with increased permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany126: 93–101.
6.
GongYToivonenPMALauOLWiersmaPA (2001) Antioxidant system level in ‘Braeburn’ apple in related to its browing disorder. Botanical Bulletin of Academica Sinica42: 259–264.
7.
HeYLLiuYLCaoWXHuaiMFXuBGHuangBG (2005) Effects of salicylic acid on heat tolerance associated with antioxidant metabolism in Kentucky bluegrass. Crop Science45: 988–995.
8.
JandaTSzalaiGRios-GonzalezKVeiszOPaldiE (2003) Comparative study of frost tolerance and antioxidant activity in cereals. Plant Science164: 301–306.
9.
KangGWangCSunGWangZ (2003) Salicylic acid changes activities of H2O2 metabolizing enzymes and increases the chilling tolerance of banana seedlings. Environmental and Experimental Botany50: 9–15.
10.
LeshemYYHaramatyEIluzDMalikZSoferYRoitmanLLeshemY (1997) Effect of stress nitric oxide (NO): interaction between chlorophyll fluorescence, galactolipid fluidity and lipoxygenese activity. Plant Physiology and Biochemistry35: 573–579.
11.
LiCZJiaoiJWangiGX (2004) The important roles of reactive oxygen species in the relationship between ethylene and polyamines in leaves of spring wheat seedlings under root osmotic stress. Plant Science166: 303–315.
12.
LiuYJiangHZhaoZAnL (2010) Nitric oxide synthase like activity-dependet nitric oxide production protects against chilling- induced oxidative damage in Chorispora bungeana suspension cultured cells. Plant physiology and biochemistry48: 936–944.
13.
LiuYWuRWanQXieGBiY (2007) Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean roots. Plant Cell Physiology48: 511–522.
14.
MahajanSTutejaN (2005) Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics444: 139–158.
15.
Mata-GarciaCLamattinaL (2001) Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiology126: 1196–1204.
16.
MutluSAticiÖEsimNMeteE (2011) Essential oils of catmint (Nepeta meyeri) induce oxidative stress in early seedlings of various weed species. Acta Physiologiae Plantarum33(3): 943–951.
17.
NeillSJDesikanRHancockJT (2003) Nitric oxide signalling in plants. New Phytologist159: 1469–1481.
18.
OkudaTMatsudaYYamanakaASagisakaS (1991) Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment. Plant Physiology97: 1265–1267.
19.
PasqualiniSMeierSGehringCMadeoLFornaciariMRomanoB. (2009) Ozone and nitric oxide induce cGMP-dependent and -independent transcription of defence genes in tobacco. New Phytologist181: 860–870.
20.
Rodriguez-SerranoMRomero-PuertasMCZabalzaACorpasFJGomezMDel- RioLA. (2006) Cadmium effect on oxidative metabolism of pea (Pisum sativum L.) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo. Plant Cell and Environment29: 1532–1544.
21.
ScebbaFSebastianiLVitaglianoC (1999) Protective enzymes against oxygen species in wheat (Triticum aestivum L.) seedlings: responses to cold acclimation. Journal of Plant Physiology155: 762–768.
22.
ShiQHDingFWangXFWeiM (2007) Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress. Plant Physiology and Biochemistry45: 542–550.
23.
ShiSWangGWangYZhangLZhangLX (2005) Protective effect of nitric oxide against oxidative stress under ultraviolet-B radiation. Nitric Oxide13: 1–9.
24.
SiddiquiMHAl whaibeMHBasalahMO (2010) Role of nitric oxide in tolerance of plants to abiotic stress. Protoplasma248: 447–455.
25.
SinghHPBatishDRKaurGAroraKKohliRK (2008) Nitric oxide (as sodium nitroprusside) supplementation ameliorates Cd toxicity in hydroponically grown wheat roots. Environmental and Experimental Botany63: 158–167.
26.
SongLDingWZhaoMSunBZhangL (2006) Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed. Plant Science171: 449–458.
27.
TasginEAticiONalbantogluBPopovaPL (2006) Effects of salicylic acid and cold treatments on protein levels and on the activities of antioxidant enzymes in the apoplast of winter wheat leaves. Phytochemistry67: 710–715.
28.
TewariRKHahnEJPaekKY (2008) Modulation of copper toxicity-induced oxidative damage by nitric oxide supply in the adventitious roots of Panax ginseng. Plant Cell Reports27: 171–181.
29.
TianQYSunDHZhaoMGZhangWH (2007) Inhibition of nitric oxide synthase (NOS) underlies aluminum-induced inhibition of root elongation in Hibiscus moscheutos. New Phytologist174: 322–331.
30.
YeeYTamNFYWongYSLuCY (2002) Growth and physiological responses of two mangrove species (Bruguira gymnorrhiza and Kandelia candel) to water logging. Environmental and Experimental Botany49: 209–221.
31.
ZhangFWangYYangYWuHWangDLiuJ (2007) Involvement of hydrogen peroxide and nitric oxide in salt resistance in the calluses from Populus euphratica. Plant Cell and Environment30: 775–785.
32.
ZhaoMGChenLZhangLLZhangWH (2009) Nitric reductase-dependent nitric oxide production is involved in cold acclimation and freezing tolerance in arabidopsis. Plant Physiology151: 755–767.
33.
ZhaoLHeJXWangXMZhangLX (2008) Nitric oxide protects against polyethylene glycol-induced oxidative damage in two ecotypes of reed suspension cultures. Journal of Plant Physiology165: 182–191.
34.
ZhaoLQZhangFGuoJKYangYLLiBBZhangLX (2004) Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed. Plant Physiology134: 849–857.
