Two methods for constructing ZIF-8 nanomaterials with good bio compatibility and robust antibacterial applied to biomedical

Metal-organic framework materials not only possess porous structures, but also have excellent antibacterial properties. It is of great practical significance to prepare new antibacterial materials with excellent antibacterial effect by metal-organic framework materials. In our study, Zeolitic Imidazolate Framework-8 (ZIF-8) nanomaterials with antibacterial properties were prepared via the solvent method and diethanolamine template method. The materials were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), cold field-emission scanning electron microscope (SEM), transmission electron microscopy (TEM), N₂ adsorption-desorption experiment, antibacterial experiment, and biocompatibility experiment. Results showed that ZIF-8 prepared by solvent method has a more typical hexagonal structure, larger specific surface area, and smaller pore size, and the values are 1812.07 m²g⁻¹ and 2.2412 nm, respectively. At the same time, the materials prepared by the two methods have excellent antibacterial properties, and exhibit good biocompatibility at low concentrations, the antibacterial activity against Escherichia coli and Staphylococcus aureus are higher than 95%, and the cell viabilities of the selected five material concentrations of 12.5 µg mL⁻¹, 25 µg mL⁻¹, 50 µg mL⁻¹, 100 µg mL⁻¹ and 200 µg mL⁻¹ are more than 70%. Therefore, this study provides a feasible method for preparing Nano-scale antibacterial functional particles, and it is of great significance to broaden the application field of ZIF-8 materials and prepare ZIF-8 drug-delivery functional materials.