Local Lymph Node Data for the Evaluation of Skin Sensitization Alternatives: A Second Compilation

Abstract

Background:

Development, evaluation and validation of alternatives to skin sensitisation testing require the availability of reliable databases with which comparative analyses can be conducted to establish performance characteristics. To facilitate this we have published previously a database comprising results from local lymph node assays (LLNAs) conducted with 211 chemicals. That database embraced a substantial range of chemistry, and of relative skin sensitising potency, and has found application in the assessment of new or refined methods.

Objective:

In this paper we describe a second compilation to extend the LLNA database.

Methods:

This second data compilation was derived from previously conducted LLNA studies involving an additional 108 chemicals. In addition, the first database contained a small number of inaccuracies, affecting results recorded with a few chemicals. In this paper these have been corrected.

Results:

The inclusion of 108 new substances has served to extend and consolidate the areas of chemistry covered by the database. In addition, the entire dataset was evaluated for pre and prohaptens which will facilitate the choice of chemicals for alternative assay developments.

Conclusions:

It is anticipated that the new revised and extended database totalling over 300 chemicals will now serve as the primary resource to support the development and evaluation of new approaches to hazard identification and potency assessment.

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